A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.

The prediction of viral zoonosis epidemics has become a major public health issue. A profound understanding of the viral population in key animal species acting as reservoirs represents an important step towards this goal. Bats harbor diverse viruses, some of which are of particular interest because...

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Autores principales:	Laurent Dacheux, Minerva Cervantes-Gonzalez, Ghislaine Guigon, Jean-Michel Thiberge, Mathias Vandenbogaert, Corinne Maufrais, Valérie Caro, Hervé Bourhy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2e3cbdad54e44eeab25768bfb8127e59
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spelling	oai:doaj.org-article:2e3cbdad54e44eeab25768bfb8127e592021-11-18T08:35:01ZA preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.1932-620310.1371/journal.pone.0087194https://doaj.org/article/2e3cbdad54e44eeab25768bfb8127e592014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24489870/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The prediction of viral zoonosis epidemics has become a major public health issue. A profound understanding of the viral population in key animal species acting as reservoirs represents an important step towards this goal. Bats harbor diverse viruses, some of which are of particular interest because they cause severe human diseases. However, little is known about the diversity of the global population of viruses found in bats (virome). We determined the viral diversity of five different French insectivorous bat species (nine specimens in total) in close contact with humans. Sequence-independent amplification, high-throughput sequencing with Illumina technology and a dedicated bioinformatics analysis pipeline were used on pooled tissues (brain, liver and lungs). Comparisons of the sequences of contigs and unassembled reads provided a global taxonomic distribution of virus-related sequences for each sample, highlighting differences both within and between bat species. Many viral families were present in these viromes, including viruses known to infect bacteria, plants/fungi, insects or vertebrates, the most relevant being those infecting mammals (Retroviridae, Herpesviridae, Bunyaviridae, Poxviridae, Flaviviridae, Reoviridae, Bornaviridae, Picobirnaviridae). In particular, we detected several new mammalian viruses, including rotaviruses, gammaretroviruses, bornaviruses and bunyaviruses with the identification of the first bat nairovirus. These observations demonstrate that bats naturally harbor viruses from many different families, most of which infect mammals. They may therefore constitute a major reservoir of viral diversity that should be analyzed carefully, to determine the role played by bats in the spread of zoonotic viral infections.Laurent DacheuxMinerva Cervantes-GonzalezGhislaine GuigonJean-Michel ThibergeMathias VandenbogaertCorinne MaufraisValérie CaroHervé BourhyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e87194 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laurent Dacheux Minerva Cervantes-Gonzalez Ghislaine Guigon Jean-Michel Thiberge Mathias Vandenbogaert Corinne Maufrais Valérie Caro Hervé Bourhy A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.
description	The prediction of viral zoonosis epidemics has become a major public health issue. A profound understanding of the viral population in key animal species acting as reservoirs represents an important step towards this goal. Bats harbor diverse viruses, some of which are of particular interest because they cause severe human diseases. However, little is known about the diversity of the global population of viruses found in bats (virome). We determined the viral diversity of five different French insectivorous bat species (nine specimens in total) in close contact with humans. Sequence-independent amplification, high-throughput sequencing with Illumina technology and a dedicated bioinformatics analysis pipeline were used on pooled tissues (brain, liver and lungs). Comparisons of the sequences of contigs and unassembled reads provided a global taxonomic distribution of virus-related sequences for each sample, highlighting differences both within and between bat species. Many viral families were present in these viromes, including viruses known to infect bacteria, plants/fungi, insects or vertebrates, the most relevant being those infecting mammals (Retroviridae, Herpesviridae, Bunyaviridae, Poxviridae, Flaviviridae, Reoviridae, Bornaviridae, Picobirnaviridae). In particular, we detected several new mammalian viruses, including rotaviruses, gammaretroviruses, bornaviruses and bunyaviruses with the identification of the first bat nairovirus. These observations demonstrate that bats naturally harbor viruses from many different families, most of which infect mammals. They may therefore constitute a major reservoir of viral diversity that should be analyzed carefully, to determine the role played by bats in the spread of zoonotic viral infections.
format	article
author	Laurent Dacheux Minerva Cervantes-Gonzalez Ghislaine Guigon Jean-Michel Thiberge Mathias Vandenbogaert Corinne Maufrais Valérie Caro Hervé Bourhy
author_facet	Laurent Dacheux Minerva Cervantes-Gonzalez Ghislaine Guigon Jean-Michel Thiberge Mathias Vandenbogaert Corinne Maufrais Valérie Caro Hervé Bourhy
author_sort	Laurent Dacheux
title	A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.
title_short	A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.
title_full	A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.
title_fullStr	A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.
title_full_unstemmed	A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.
title_sort	preliminary study of viral metagenomics of french bat species in contact with humans: identification of new mammalian viruses.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/2e3cbdad54e44eeab25768bfb8127e59
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A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.

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