FABP4 dynamics in obesity: discrepancies in adipose tissue and liver expression regarding circulating plasma levels.

<h4>Background</h4>FABP4 is predominantly expressed in adipose tissue, and its circulating levels are linked with obesity and a poor atherogenic profile.<h4>Objective</h4>In patients with a wide BMI range, we analyze FABP4 expression in adipose and hepatic tissues in the sett...

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Autores principales: María Isabel Queipo-Ortuño, Xavier Escoté, Victoria Ceperuelo-Mallafré, Lourdes Garrido-Sanchez, Merce Miranda, Mercedes Clemente-Postigo, Rafael Pérez-Pérez, Belen Peral, Fernando Cardona, Jose Manuel Fernández-Real, Francisco J Tinahones, Joan Vendrell
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Publicado: Public Library of Science (PLoS) 2012
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spelling oai:doaj.org-article:2e3dc68d5895411d9b15fc98dab7db392021-11-18T08:10:05ZFABP4 dynamics in obesity: discrepancies in adipose tissue and liver expression regarding circulating plasma levels.1932-620310.1371/journal.pone.0048605https://doaj.org/article/2e3dc68d5895411d9b15fc98dab7db392012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23139800/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>FABP4 is predominantly expressed in adipose tissue, and its circulating levels are linked with obesity and a poor atherogenic profile.<h4>Objective</h4>In patients with a wide BMI range, we analyze FABP4 expression in adipose and hepatic tissues in the settings of obesity and insulin resistance. Associations between FABP4 expression in adipose tissue and the FABP4 plasma level as well as the main adipogenic and lipolytic genes expressed in adipose tissue were also analyzed.<h4>Methods</h4>The expression of several lipogenic, lipolytic, PPAR family and FABP family genes was analyzed by real time PCR. FABP4 protein expression in total adipose tissues and its fractions were determined by western blot.<h4>Results</h4>In obesity FABP4 expression was down-regulated (at both mRNA and protein levels), with its levels mainly predicted by ATGL and inversely by the HOMA-IR index. The BMI appeared as the only determinant of the FABP4 variation in both adipose tissue depots. FABP4 plasma levels showed a significant progressive increase according to BMI but no association was detected between FABP4 circulating levels and SAT or VAT FABP4 gene expression. The gene expression of FABP1, FABP4 and FABP5 in hepatic tissue was significantly higher in tissue from the obese IR patients compared to the non-IR group.<h4>Conclusion</h4>The inverse pattern in FABP4 expression between adipose and hepatic tissue observed in morbid obese patients, regarding the IR context, suggests that both tissues may act in a balanced manner. These differences may help us to understand the discrepancies between circulating plasma levels and adipose tissue expression in obesity.María Isabel Queipo-OrtuñoXavier EscotéVictoria Ceperuelo-MallafréLourdes Garrido-SanchezMerce MirandaMercedes Clemente-PostigoRafael Pérez-PérezBelen PeralFernando CardonaJose Manuel Fernández-RealFrancisco J TinahonesJoan VendrellPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48605 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
María Isabel Queipo-Ortuño
Xavier Escoté
Victoria Ceperuelo-Mallafré
Lourdes Garrido-Sanchez
Merce Miranda
Mercedes Clemente-Postigo
Rafael Pérez-Pérez
Belen Peral
Fernando Cardona
Jose Manuel Fernández-Real
Francisco J Tinahones
Joan Vendrell
FABP4 dynamics in obesity: discrepancies in adipose tissue and liver expression regarding circulating plasma levels.
description <h4>Background</h4>FABP4 is predominantly expressed in adipose tissue, and its circulating levels are linked with obesity and a poor atherogenic profile.<h4>Objective</h4>In patients with a wide BMI range, we analyze FABP4 expression in adipose and hepatic tissues in the settings of obesity and insulin resistance. Associations between FABP4 expression in adipose tissue and the FABP4 plasma level as well as the main adipogenic and lipolytic genes expressed in adipose tissue were also analyzed.<h4>Methods</h4>The expression of several lipogenic, lipolytic, PPAR family and FABP family genes was analyzed by real time PCR. FABP4 protein expression in total adipose tissues and its fractions were determined by western blot.<h4>Results</h4>In obesity FABP4 expression was down-regulated (at both mRNA and protein levels), with its levels mainly predicted by ATGL and inversely by the HOMA-IR index. The BMI appeared as the only determinant of the FABP4 variation in both adipose tissue depots. FABP4 plasma levels showed a significant progressive increase according to BMI but no association was detected between FABP4 circulating levels and SAT or VAT FABP4 gene expression. The gene expression of FABP1, FABP4 and FABP5 in hepatic tissue was significantly higher in tissue from the obese IR patients compared to the non-IR group.<h4>Conclusion</h4>The inverse pattern in FABP4 expression between adipose and hepatic tissue observed in morbid obese patients, regarding the IR context, suggests that both tissues may act in a balanced manner. These differences may help us to understand the discrepancies between circulating plasma levels and adipose tissue expression in obesity.
format article
author María Isabel Queipo-Ortuño
Xavier Escoté
Victoria Ceperuelo-Mallafré
Lourdes Garrido-Sanchez
Merce Miranda
Mercedes Clemente-Postigo
Rafael Pérez-Pérez
Belen Peral
Fernando Cardona
Jose Manuel Fernández-Real
Francisco J Tinahones
Joan Vendrell
author_facet María Isabel Queipo-Ortuño
Xavier Escoté
Victoria Ceperuelo-Mallafré
Lourdes Garrido-Sanchez
Merce Miranda
Mercedes Clemente-Postigo
Rafael Pérez-Pérez
Belen Peral
Fernando Cardona
Jose Manuel Fernández-Real
Francisco J Tinahones
Joan Vendrell
author_sort María Isabel Queipo-Ortuño
title FABP4 dynamics in obesity: discrepancies in adipose tissue and liver expression regarding circulating plasma levels.
title_short FABP4 dynamics in obesity: discrepancies in adipose tissue and liver expression regarding circulating plasma levels.
title_full FABP4 dynamics in obesity: discrepancies in adipose tissue and liver expression regarding circulating plasma levels.
title_fullStr FABP4 dynamics in obesity: discrepancies in adipose tissue and liver expression regarding circulating plasma levels.
title_full_unstemmed FABP4 dynamics in obesity: discrepancies in adipose tissue and liver expression regarding circulating plasma levels.
title_sort fabp4 dynamics in obesity: discrepancies in adipose tissue and liver expression regarding circulating plasma levels.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/2e3dc68d5895411d9b15fc98dab7db39
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