Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis

Abstract Sand flies inject saliva while feeding in the vertebrate host and anti-saliva antibodies can be used as biomarkers of exposure to Leishmania vectors. We expressed recombinant salivary proteins from Lutzomyia intermedia, a vector of Leishmania braziliensis, and evaluated the seroreactivity i...

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Autores principales:	Augusto M. Carvalho, Kiyoshi F. Fukutani, Rohit Sharma, Rebecca P. Curvelo, José Carlos Miranda, Aldina Barral, Edgar M. Carvalho, Jesus G. Valenzuela, Fabiano Oliveira, Camila I. de Oliveira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2e3f014686eb4b2794c25c0f97610a2f
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spelling	oai:doaj.org-article:2e3f014686eb4b2794c25c0f97610a2f2021-12-02T15:05:19ZSeroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis10.1038/s41598-017-03345-02045-2322https://doaj.org/article/2e3f014686eb4b2794c25c0f97610a2f2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03345-0https://doaj.org/toc/2045-2322Abstract Sand flies inject saliva while feeding in the vertebrate host and anti-saliva antibodies can be used as biomarkers of exposure to Leishmania vectors. We expressed recombinant salivary proteins from Lutzomyia intermedia, a vector of Leishmania braziliensis, and evaluated the seroreactivity in exposed individuals in search for exposure markers. We found a strong correlation among positive serology to recombinant proteins LinB-13, 26, 15, 21 and to salivary proteins: rLinB-13 was the top performing molecule; IgG4 was the most predominant antibody subclass and antibodies to rLinB-13 did not cross react with Lu. longipalpis salivary proteins. By evaluating a cohort of contacts of CL patients, we confirmed that rLinB-13, an antigen 5-related protein, is a marker of exposure to Lu. intermedia with high degree of accuracy. In a 5-year follow up, we determined that individuals who developed CL presented higher anti-rLinB13 IgG responses, before the appearance of clinical symptoms. They also presented a lower frequency of cellular responses to the parasite (DTH). Our results show that seroconversion to a salivary molecule, rLinB-13, is a marker of risk for CL development caused by Leishmania braziliensis. This highlight the possibility of developing tools based on vector molecules to manage the disease in endemic areas.Augusto M. CarvalhoKiyoshi F. FukutaniRohit SharmaRebecca P. CurveloJosé Carlos MirandaAldina BarralEdgar M. CarvalhoJesus G. ValenzuelaFabiano OliveiraCamila I. de OliveiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Augusto M. Carvalho Kiyoshi F. Fukutani Rohit Sharma Rebecca P. Curvelo José Carlos Miranda Aldina Barral Edgar M. Carvalho Jesus G. Valenzuela Fabiano Oliveira Camila I. de Oliveira Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
description	Abstract Sand flies inject saliva while feeding in the vertebrate host and anti-saliva antibodies can be used as biomarkers of exposure to Leishmania vectors. We expressed recombinant salivary proteins from Lutzomyia intermedia, a vector of Leishmania braziliensis, and evaluated the seroreactivity in exposed individuals in search for exposure markers. We found a strong correlation among positive serology to recombinant proteins LinB-13, 26, 15, 21 and to salivary proteins: rLinB-13 was the top performing molecule; IgG4 was the most predominant antibody subclass and antibodies to rLinB-13 did not cross react with Lu. longipalpis salivary proteins. By evaluating a cohort of contacts of CL patients, we confirmed that rLinB-13, an antigen 5-related protein, is a marker of exposure to Lu. intermedia with high degree of accuracy. In a 5-year follow up, we determined that individuals who developed CL presented higher anti-rLinB13 IgG responses, before the appearance of clinical symptoms. They also presented a lower frequency of cellular responses to the parasite (DTH). Our results show that seroconversion to a salivary molecule, rLinB-13, is a marker of risk for CL development caused by Leishmania braziliensis. This highlight the possibility of developing tools based on vector molecules to manage the disease in endemic areas.
format	article
author	Augusto M. Carvalho Kiyoshi F. Fukutani Rohit Sharma Rebecca P. Curvelo José Carlos Miranda Aldina Barral Edgar M. Carvalho Jesus G. Valenzuela Fabiano Oliveira Camila I. de Oliveira
author_facet	Augusto M. Carvalho Kiyoshi F. Fukutani Rohit Sharma Rebecca P. Curvelo José Carlos Miranda Aldina Barral Edgar M. Carvalho Jesus G. Valenzuela Fabiano Oliveira Camila I. de Oliveira
author_sort	Augusto M. Carvalho
title	Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
title_short	Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
title_full	Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
title_fullStr	Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
title_full_unstemmed	Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
title_sort	seroconversion to lutzomyia intermedia linb-13 as a biomarker for developing cutaneous leishmaniasis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2e3f014686eb4b2794c25c0f97610a2f
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Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis

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