3D cell culture using a clinostat reproduces microgravity-induced skin changes

Abstract Exposure to microgravity affects human physiology in various ways, and astronauts frequently report skin-related problems. Skin rash and irritation are frequent complaints during space missions, and skin thinning has also been reported after returning to Earth. However, spaceflight missions...

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Autores principales:	Dong Hyun Choi, Byoungjun Jeon, Min Hyuk Lim, Dong Hun Lee, Sang-Kyu Ye, Seung-Yong Jeong, Sungwan Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biotechnology TP248.13-248.65 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/2e404795314b4bc6af0b2f2c684ae617
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spelling	oai:doaj.org-article:2e404795314b4bc6af0b2f2c684ae6172021-12-02T17:51:20Z3D cell culture using a clinostat reproduces microgravity-induced skin changes10.1038/s41526-021-00148-62373-8065https://doaj.org/article/2e404795314b4bc6af0b2f2c684ae6172021-06-01T00:00:00Zhttps://doi.org/10.1038/s41526-021-00148-6https://doaj.org/toc/2373-8065Abstract Exposure to microgravity affects human physiology in various ways, and astronauts frequently report skin-related problems. Skin rash and irritation are frequent complaints during space missions, and skin thinning has also been reported after returning to Earth. However, spaceflight missions for studying the physiological changes in microgravity are impractical. Thus, we used a previously developed 3D clinostat to simulate a microgravity environment and investigate whether physiological changes of the skin can be reproduced in a 3D in vitro setting. Our results showed that under time-averaged simulated microgravity (taSMG), the thickness of the endothelial cell arrangement increased by up to 59.75%, indicating skin irritation due to vasodilation, and that the diameter of keratinocytes and fibroblast co-cultured spheroids decreased by 6.66%, representing skin thinning. The α1 chain of type I collagen was upregulated, while the connective tissue growth factor was downregulated under taSMG. Cytokeratin-10 expression was significantly increased in the taSMG environment. The clinostat-based 3D culture system can reproduce physiological changes in the skin similar to those under microgravity, providing insight for understanding the effects of microgravity on human health before space exploration.Dong Hyun ChoiByoungjun JeonMin Hyuk LimDong Hun LeeSang-Kyu YeSeung-Yong JeongSungwan KimNature PortfolioarticleBiotechnologyTP248.13-248.65PhysiologyQP1-981ENnpj Microgravity, Vol 7, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65 Physiology QP1-981
spellingShingle	Biotechnology TP248.13-248.65 Physiology QP1-981 Dong Hyun Choi Byoungjun Jeon Min Hyuk Lim Dong Hun Lee Sang-Kyu Ye Seung-Yong Jeong Sungwan Kim 3D cell culture using a clinostat reproduces microgravity-induced skin changes
description	Abstract Exposure to microgravity affects human physiology in various ways, and astronauts frequently report skin-related problems. Skin rash and irritation are frequent complaints during space missions, and skin thinning has also been reported after returning to Earth. However, spaceflight missions for studying the physiological changes in microgravity are impractical. Thus, we used a previously developed 3D clinostat to simulate a microgravity environment and investigate whether physiological changes of the skin can be reproduced in a 3D in vitro setting. Our results showed that under time-averaged simulated microgravity (taSMG), the thickness of the endothelial cell arrangement increased by up to 59.75%, indicating skin irritation due to vasodilation, and that the diameter of keratinocytes and fibroblast co-cultured spheroids decreased by 6.66%, representing skin thinning. The α1 chain of type I collagen was upregulated, while the connective tissue growth factor was downregulated under taSMG. Cytokeratin-10 expression was significantly increased in the taSMG environment. The clinostat-based 3D culture system can reproduce physiological changes in the skin similar to those under microgravity, providing insight for understanding the effects of microgravity on human health before space exploration.
format	article
author	Dong Hyun Choi Byoungjun Jeon Min Hyuk Lim Dong Hun Lee Sang-Kyu Ye Seung-Yong Jeong Sungwan Kim
author_facet	Dong Hyun Choi Byoungjun Jeon Min Hyuk Lim Dong Hun Lee Sang-Kyu Ye Seung-Yong Jeong Sungwan Kim
author_sort	Dong Hyun Choi
title	3D cell culture using a clinostat reproduces microgravity-induced skin changes
title_short	3D cell culture using a clinostat reproduces microgravity-induced skin changes
title_full	3D cell culture using a clinostat reproduces microgravity-induced skin changes
title_fullStr	3D cell culture using a clinostat reproduces microgravity-induced skin changes
title_full_unstemmed	3D cell culture using a clinostat reproduces microgravity-induced skin changes
title_sort	3d cell culture using a clinostat reproduces microgravity-induced skin changes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2e404795314b4bc6af0b2f2c684ae617
work_keys_str_mv	AT donghyunchoi 3dcellcultureusingaclinostatreproducesmicrogravityinducedskinchanges AT byoungjunjeon 3dcellcultureusingaclinostatreproducesmicrogravityinducedskinchanges AT minhyuklim 3dcellcultureusingaclinostatreproducesmicrogravityinducedskinchanges AT donghunlee 3dcellcultureusingaclinostatreproducesmicrogravityinducedskinchanges AT sangkyuye 3dcellcultureusingaclinostatreproducesmicrogravityinducedskinchanges AT seungyongjeong 3dcellcultureusingaclinostatreproducesmicrogravityinducedskinchanges AT sungwankim 3dcellcultureusingaclinostatreproducesmicrogravityinducedskinchanges
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3D cell culture using a clinostat reproduces microgravity-induced skin changes

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