Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology

Levetiracetam (LEV) is a broad-spectrum and widely used antiepileptic drug that also has neuroprotective effects in different neurological conditions. Given its complex interaction with neuronal physiology, a better comprehension of LEV effects on neurons activity is needed. Microelectrode arrays (M...

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Autores principales:	Andrea Di Credico, Giulia Gaggi, Pascal Izzicupo, Laura Ferri, Laura Bonanni, Giovanni Iannetti, Angela Di Baldassarre, Barbara Ghinassi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microelectrode array biosensors real-time monitoring iPSC-derived neurons drug screening Levetiracetam Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/2e521f1dc96345a8afbbba09092dc738
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spelling	oai:doaj.org-article:2e521f1dc96345a8afbbba09092dc7382021-11-25T16:55:34ZReal-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology10.3390/bios111104502079-6374https://doaj.org/article/2e521f1dc96345a8afbbba09092dc7382021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/450https://doaj.org/toc/2079-6374Levetiracetam (LEV) is a broad-spectrum and widely used antiepileptic drug that also has neuroprotective effects in different neurological conditions. Given its complex interaction with neuronal physiology, a better comprehension of LEV effects on neurons activity is needed. Microelectrode arrays (MEAs) represent an advanced technology for the non-invasive study of electrophysiological activity of neuronal cell cultures. In this study, we exploited the Maestro Edge MEA system, a platform that allows a deep analysis of the electrical network behavior, to study the electrophysiological effect of LEV on a mixed population of human neurons (glutamatergic, GABAergic and dopaminergic neurons, and astrocytes). We found that LEV significantly affected different variables such as spiking, single-electrode bursting, and network bursting activity, with a pronounced effect after 15 min. Moreover, neuronal cell culture completely rescued its baseline activity after 24 h without LEV. In summary, MEA technology confirmed its high sensitivity in detecting drug-induced electrophysiological modifications. Moreover, our results allow one to extend the knowledge on the electrophysiological effects of LEV on the complex neuronal population that resembles the human cortex.Andrea Di CredicoGiulia GaggiPascal IzzicupoLaura FerriLaura BonanniGiovanni IannettiAngela Di BaldassarreBarbara GhinassiMDPI AGarticlemicroelectrode arraybiosensorsreal-time monitoringiPSC-derived neuronsdrug screeningLevetiracetamBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 450, p 450 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microelectrode array biosensors real-time monitoring iPSC-derived neurons drug screening Levetiracetam Biotechnology TP248.13-248.65
spellingShingle	microelectrode array biosensors real-time monitoring iPSC-derived neurons drug screening Levetiracetam Biotechnology TP248.13-248.65 Andrea Di Credico Giulia Gaggi Pascal Izzicupo Laura Ferri Laura Bonanni Giovanni Iannetti Angela Di Baldassarre Barbara Ghinassi Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology
description	Levetiracetam (LEV) is a broad-spectrum and widely used antiepileptic drug that also has neuroprotective effects in different neurological conditions. Given its complex interaction with neuronal physiology, a better comprehension of LEV effects on neurons activity is needed. Microelectrode arrays (MEAs) represent an advanced technology for the non-invasive study of electrophysiological activity of neuronal cell cultures. In this study, we exploited the Maestro Edge MEA system, a platform that allows a deep analysis of the electrical network behavior, to study the electrophysiological effect of LEV on a mixed population of human neurons (glutamatergic, GABAergic and dopaminergic neurons, and astrocytes). We found that LEV significantly affected different variables such as spiking, single-electrode bursting, and network bursting activity, with a pronounced effect after 15 min. Moreover, neuronal cell culture completely rescued its baseline activity after 24 h without LEV. In summary, MEA technology confirmed its high sensitivity in detecting drug-induced electrophysiological modifications. Moreover, our results allow one to extend the knowledge on the electrophysiological effects of LEV on the complex neuronal population that resembles the human cortex.
format	article
author	Andrea Di Credico Giulia Gaggi Pascal Izzicupo Laura Ferri Laura Bonanni Giovanni Iannetti Angela Di Baldassarre Barbara Ghinassi
author_facet	Andrea Di Credico Giulia Gaggi Pascal Izzicupo Laura Ferri Laura Bonanni Giovanni Iannetti Angela Di Baldassarre Barbara Ghinassi
author_sort	Andrea Di Credico
title	Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology
title_short	Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology
title_full	Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology
title_fullStr	Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology
title_full_unstemmed	Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology
title_sort	real-time monitoring of levetiracetam effect on the electrophysiology of an heterogenous human ipsc-derived neuronal cell culture using microelectrode array technology
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2e521f1dc96345a8afbbba09092dc738
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Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology

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