DEVELOPMENT OF DIRECT BIOAUTOGRAPHY AS REFERENCE METHOD FOR TESTING ANTIMICROBIAL ACTIVITY OF GENTAMICIN AGAINST <I>Escherichia coli</I>

Bioautography is a fast and simple technique which combines the advantages of thin layer chromatography separation and antimicrobial activity detection. This technique displays directly antimicrobial fraction spots from a mixture. Efficacy determination of the technique improves the isolation of ant...

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Autores principales:	Jhonny COLORADO R., Elkin GALEANO J., Alejandro MARTÍNEZ M.
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2009
Materias:	Direct bioautography gentamicin Escherichia coli. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/2e5b95d334ab4394937d16b6d85627c0
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spelling	oai:doaj.org-article:2e5b95d334ab4394937d16b6d85627c02021-11-19T04:21:29ZDEVELOPMENT OF DIRECT BIOAUTOGRAPHY AS REFERENCE METHOD FOR TESTING ANTIMICROBIAL ACTIVITY OF GENTAMICIN AGAINST <I>Escherichia coli</I>0121-40042145-2660https://doaj.org/article/2e5b95d334ab4394937d16b6d85627c02009-02-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/595https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660Bioautography is a fast and simple technique which combines the advantages of thin layer chromatography separation and antimicrobial activity detection. This technique displays directly antimicrobial fraction spots from a mixture. Efficacy determination of the technique improves the isolation of antimicrobial substances from complex mixtures. This article describes how bioautography is useful to quantify antimicrobial activity of amino glycoside gentamicin, against pathogen Escherichia coli (E. coli). Quantitative analysis is verified measuring diffusion halos diameters and applying different concentrations of gentamicin on tlc plates. Volume effect is measured on variability of results. Differences between diffusion halos and microbial inhibition are analyzed too. Results show how antibiotic diffusion on tlc plates is a determining factor, and there is a concentration range where strong interactions between measured variables led to establish a method to be validated for detecting and quantifying antimicrobial substances from complex mixtures. Jhonny COLORADO R.Elkin GALEANO J.Alejandro MARTÍNEZ M.Universidad de AntioquiaarticleDirect bioautographygentamicinEscherichia coli.Food processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 14, Iss 1 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Direct bioautography gentamicin Escherichia coli. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Direct bioautography gentamicin Escherichia coli. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Jhonny COLORADO R. Elkin GALEANO J. Alejandro MARTÍNEZ M. DEVELOPMENT OF DIRECT BIOAUTOGRAPHY AS REFERENCE METHOD FOR TESTING ANTIMICROBIAL ACTIVITY OF GENTAMICIN AGAINST <I>Escherichia coli</I>
description	Bioautography is a fast and simple technique which combines the advantages of thin layer chromatography separation and antimicrobial activity detection. This technique displays directly antimicrobial fraction spots from a mixture. Efficacy determination of the technique improves the isolation of antimicrobial substances from complex mixtures. This article describes how bioautography is useful to quantify antimicrobial activity of amino glycoside gentamicin, against pathogen Escherichia coli (E. coli). Quantitative analysis is verified measuring diffusion halos diameters and applying different concentrations of gentamicin on tlc plates. Volume effect is measured on variability of results. Differences between diffusion halos and microbial inhibition are analyzed too. Results show how antibiotic diffusion on tlc plates is a determining factor, and there is a concentration range where strong interactions between measured variables led to establish a method to be validated for detecting and quantifying antimicrobial substances from complex mixtures.
format	article
author	Jhonny COLORADO R. Elkin GALEANO J. Alejandro MARTÍNEZ M.
author_facet	Jhonny COLORADO R. Elkin GALEANO J. Alejandro MARTÍNEZ M.
author_sort	Jhonny COLORADO R.
title	DEVELOPMENT OF DIRECT BIOAUTOGRAPHY AS REFERENCE METHOD FOR TESTING ANTIMICROBIAL ACTIVITY OF GENTAMICIN AGAINST <I>Escherichia coli</I>
title_short	DEVELOPMENT OF DIRECT BIOAUTOGRAPHY AS REFERENCE METHOD FOR TESTING ANTIMICROBIAL ACTIVITY OF GENTAMICIN AGAINST <I>Escherichia coli</I>
title_full	DEVELOPMENT OF DIRECT BIOAUTOGRAPHY AS REFERENCE METHOD FOR TESTING ANTIMICROBIAL ACTIVITY OF GENTAMICIN AGAINST <I>Escherichia coli</I>
title_fullStr	DEVELOPMENT OF DIRECT BIOAUTOGRAPHY AS REFERENCE METHOD FOR TESTING ANTIMICROBIAL ACTIVITY OF GENTAMICIN AGAINST <I>Escherichia coli</I>
title_full_unstemmed	DEVELOPMENT OF DIRECT BIOAUTOGRAPHY AS REFERENCE METHOD FOR TESTING ANTIMICROBIAL ACTIVITY OF GENTAMICIN AGAINST <I>Escherichia coli</I>
title_sort	development of direct bioautography as reference method for testing antimicrobial activity of gentamicin against <i>escherichia coli</i>
publisher	Universidad de Antioquia
publishDate	2009
url	https://doaj.org/article/2e5b95d334ab4394937d16b6d85627c0
work_keys_str_mv	AT jhonnycolorador developmentofdirectbioautographyasreferencemethodfortestingantimicrobialactivityofgentamicinagainstiescherichiacolii AT elkingaleanoj developmentofdirectbioautographyasreferencemethodfortestingantimicrobialactivityofgentamicinagainstiescherichiacolii AT alejandromartinezm developmentofdirectbioautographyasreferencemethodfortestingantimicrobialactivityofgentamicinagainstiescherichiacolii
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DEVELOPMENT OF DIRECT BIOAUTOGRAPHY AS REFERENCE METHOD FOR TESTING ANTIMICROBIAL ACTIVITY OF GENTAMICIN AGAINST <I>Escherichia coli</I>

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