Genome-wide identification and expression profiling of the COBRA-like genes reveal likely roles in stem strength in rapeseed (Brassica napus L.).

The COBRA-like (COBL) genes play key roles in cell anisotropic expansion and the orientation of microfibrils. Mutations in these genes cause the brittle stem and induce pathogen responsive phenotypes in Arabidopsis and several crop plants. In this study, an in silico genome-wide analysis was perform...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qian Yang, Shan Wang, Hao Chen, Liang You, Fangying Liu, Zhongsong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2e5bdd2151d240b385a625614a747326
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2e5bdd2151d240b385a625614a747326
record_format	dspace
spelling	oai:doaj.org-article:2e5bdd2151d240b385a625614a7473262021-12-02T20:16:08ZGenome-wide identification and expression profiling of the COBRA-like genes reveal likely roles in stem strength in rapeseed (Brassica napus L.).1932-620310.1371/journal.pone.0260268https://doaj.org/article/2e5bdd2151d240b385a625614a7473262021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0260268https://doaj.org/toc/1932-6203The COBRA-like (COBL) genes play key roles in cell anisotropic expansion and the orientation of microfibrils. Mutations in these genes cause the brittle stem and induce pathogen responsive phenotypes in Arabidopsis and several crop plants. In this study, an in silico genome-wide analysis was performed to identify the COBL family members in Brassica. We identified 44, 20 and 23 COBL genes in B. napus and its diploid progenitor species B. rapa and B. oleracea, respectively. All the predicted COBL genes were phylogenetically clustered into two groups: the AtCOB group and the AtCOBL7 group. The conserved chromosome locations of COBLs in Arabidopsis and Brassica, together with clustering, indicated that the expansion of the COBL gene family in B. napus was primarily attributable to whole-genome triplication. Among the BnaCOBLs, 22 contained all the conserved motifs and derived from 9 of 12 subgroups. RNA-seq analysis was used to determine the tissue preferential expression patterns of various subgroups. BnaCOBL9, BnaCOBL35 and BnaCOBL41 were highly expressed in stem with high-breaking resistance, which implies these AtCOB subgroup members may be involved in stem development and stem breaking resistance of rapeseed. Our results of this study may help to elucidate the molecular properties of the COBRA gene family and provide informative clues for high stem-breaking resistance studies.Qian YangShan WangHao ChenLiang YouFangying LiuZhongsong LiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0260268 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qian Yang Shan Wang Hao Chen Liang You Fangying Liu Zhongsong Liu Genome-wide identification and expression profiling of the COBRA-like genes reveal likely roles in stem strength in rapeseed (Brassica napus L.).
description	The COBRA-like (COBL) genes play key roles in cell anisotropic expansion and the orientation of microfibrils. Mutations in these genes cause the brittle stem and induce pathogen responsive phenotypes in Arabidopsis and several crop plants. In this study, an in silico genome-wide analysis was performed to identify the COBL family members in Brassica. We identified 44, 20 and 23 COBL genes in B. napus and its diploid progenitor species B. rapa and B. oleracea, respectively. All the predicted COBL genes were phylogenetically clustered into two groups: the AtCOB group and the AtCOBL7 group. The conserved chromosome locations of COBLs in Arabidopsis and Brassica, together with clustering, indicated that the expansion of the COBL gene family in B. napus was primarily attributable to whole-genome triplication. Among the BnaCOBLs, 22 contained all the conserved motifs and derived from 9 of 12 subgroups. RNA-seq analysis was used to determine the tissue preferential expression patterns of various subgroups. BnaCOBL9, BnaCOBL35 and BnaCOBL41 were highly expressed in stem with high-breaking resistance, which implies these AtCOB subgroup members may be involved in stem development and stem breaking resistance of rapeseed. Our results of this study may help to elucidate the molecular properties of the COBRA gene family and provide informative clues for high stem-breaking resistance studies.
format	article
author	Qian Yang Shan Wang Hao Chen Liang You Fangying Liu Zhongsong Liu
author_facet	Qian Yang Shan Wang Hao Chen Liang You Fangying Liu Zhongsong Liu
author_sort	Qian Yang
title	Genome-wide identification and expression profiling of the COBRA-like genes reveal likely roles in stem strength in rapeseed (Brassica napus L.).
title_short	Genome-wide identification and expression profiling of the COBRA-like genes reveal likely roles in stem strength in rapeseed (Brassica napus L.).
title_full	Genome-wide identification and expression profiling of the COBRA-like genes reveal likely roles in stem strength in rapeseed (Brassica napus L.).
title_fullStr	Genome-wide identification and expression profiling of the COBRA-like genes reveal likely roles in stem strength in rapeseed (Brassica napus L.).
title_full_unstemmed	Genome-wide identification and expression profiling of the COBRA-like genes reveal likely roles in stem strength in rapeseed (Brassica napus L.).
title_sort	genome-wide identification and expression profiling of the cobra-like genes reveal likely roles in stem strength in rapeseed (brassica napus l.).
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/2e5bdd2151d240b385a625614a747326
work_keys_str_mv	AT qianyang genomewideidentificationandexpressionprofilingofthecobralikegenesreveallikelyrolesinstemstrengthinrapeseedbrassicanapusl AT shanwang genomewideidentificationandexpressionprofilingofthecobralikegenesreveallikelyrolesinstemstrengthinrapeseedbrassicanapusl AT haochen genomewideidentificationandexpressionprofilingofthecobralikegenesreveallikelyrolesinstemstrengthinrapeseedbrassicanapusl AT liangyou genomewideidentificationandexpressionprofilingofthecobralikegenesreveallikelyrolesinstemstrengthinrapeseedbrassicanapusl AT fangyingliu genomewideidentificationandexpressionprofilingofthecobralikegenesreveallikelyrolesinstemstrengthinrapeseedbrassicanapusl AT zhongsongliu genomewideidentificationandexpressionprofilingofthecobralikegenesreveallikelyrolesinstemstrengthinrapeseedbrassicanapusl
_version_	1718374515777470464

Genome-wide identification and expression profiling of the COBRA-like genes reveal likely roles in stem strength in rapeseed (Brassica napus L.).

Ejemplares similares