Nanostructured Cellulose–Gellan–Xyloglucan–Lysozyme Dressing Seeded with Mesenchymal Stem Cells for Deep Second-Degree Burn Treatment

Carolina Maria Costa de Oliveira Souza,1 Clayton Fernandes de Souza,2,3 Bassam Felipe Mogharbel,1 Ana Carolina Irioda,1 Celia Regina Cavichiolo Franco,4 Maria Rita Sierakowski,3 Katherine Athayde Teixeira de Carvalho1 1Stem Cell Research Laboratory, Cell Therapy and Biotechnology in Regenerative Med...

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Autores principales: Costa de Oliveira Souza CM, de Souza CF, Mogharbel BF, Irioda AC, Cavichiolo Franco CR, Sierakowski MR, Athayde Teixeira de Carvalho K
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Publicado: Dove Medical Press 2021
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spelling oai:doaj.org-article:2e7d380f1767459f8eed5f1d866f2a722021-12-02T14:18:39ZNanostructured Cellulose–Gellan–Xyloglucan–Lysozyme Dressing Seeded with Mesenchymal Stem Cells for Deep Second-Degree Burn Treatment1178-2013https://doaj.org/article/2e7d380f1767459f8eed5f1d866f2a722021-02-01T00:00:00Zhttps://www.dovepress.com/nanostructured-cellulosendashgellanndashxyloglucanndashlysozyme-dressi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Carolina Maria Costa de Oliveira Souza,1 Clayton Fernandes de Souza,2,3 Bassam Felipe Mogharbel,1 Ana Carolina Irioda,1 Celia Regina Cavichiolo Franco,4 Maria Rita Sierakowski,3 Katherine Athayde Teixeira de Carvalho1 1Stem Cell Research Laboratory, Cell Therapy and Biotechnology in Regenerative Medicine Department, Pequeno Príncipe Faculties and the Pelé Pequeno Príncipe Research Institute, Curitiba, Paraná, Brazil; 2Chemistry Undergraduate Program, School of Education and Humanities, Pontifícia Universidade Católica Do Paraná, Curitiba, Paraná, Brazil; 3BioPol, Chemistry Department, Federal University of Paraná, Curitiba, Paraná, Brazil; 4Molecular Biology Department, Federal University of Paraná, Curitiba, Paraná, BrazilCorrespondence: Katherine Athayde Teixeira de CarvalhoStem Cell Research Laboratory, Cell Therapy and Biotechnology in Regenerative Medicine Department, Pequeno Príncipe Faculties and the Pelé Pequeno Príncipe Research Institute, 1632 Avenida Silva Jardim, Box 80.240-020, Curitiba, Paraná, BrazilTel +55 41-3310-1034Email katherinecarv@gmail.comPurpose: In deep burns, wound contraction and hypertrophic scar formation can generate functional derangement and debilitation of the affected part. In order to improve the quality of healing in deep second-degree burns, we developed a new treatment in a preclinical model using nanostructured membranes seeded with mesenchymal stem cells (MSCs).Methods: Membranes were obtained by reconstitution of bacterial cellulose (reconstituted membrane [RM]) and produced by a dry-cast process, then RM was incorporated with 10% tamarind xyloglucan plus gellan gum 1:1 and 10% lysozyme (RMGT–LZ) and with 10% gellan gum and 10% lysozyme (RMG–LZ). Membrane hydrophobic/hydrophilic characteristics were investigated by static/dynamic contact-angle measurements. They were cultivated with MSCs, and cell adhesion, proliferation, and migration capacity was analyzed with MTT assays. Morphological and topographic characteristics were analyzed by scanning electron microscopy. MSC patterns in flow cytometry and differentiation into adipocytes and osteocytes were checked. In vivo assays used RMG–LZ and RMGT–LZ (with and without MSCs) in Rattus norvegicus rats submitted to burn protocol, and histological sections and collagen deposits were analyzed and immunocytochemistry assay performed.Results: In vitro results demonstrated carboxyl and amine groups made the membranes moderately hydrophobic and xyloglucan inclusion decreased wettability, favoring MSC adhesion, proliferation, and differentiation. In vivo, we obtained 40% and 60% reduction in acute/chronic inflammatory infiltrates, 96% decrease in injury area, increased vascular proliferation and collagen deposition, and complete epithelialization after 30 days. MSCs were detected in burned tissue, confirming they had homed and proliferated in vivo.Conclusion: Nanostructured cellulose–gellan–xyloglucan–lysozyme dressings, especially when seeded with MSCs, improved deep second-degree burn regeneration.Keywords: skin, cell-based therapy, transplantation, hydrocolloid dressingCosta de Oliveira Souza CMde Souza CFMogharbel BFIrioda ACCavichiolo Franco CRSierakowski MRAthayde Teixeira de Carvalho KDove Medical Pressarticleskincell based therapytransplantationhydrocolloid dressingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 833-850 (2021)
institution DOAJ
collection DOAJ
language EN
topic skin
cell based therapy
transplantation
hydrocolloid dressing
Medicine (General)
R5-920
spellingShingle skin
cell based therapy
transplantation
hydrocolloid dressing
Medicine (General)
R5-920
Costa de Oliveira Souza CM
de Souza CF
Mogharbel BF
Irioda AC
Cavichiolo Franco CR
Sierakowski MR
Athayde Teixeira de Carvalho K
Nanostructured Cellulose–Gellan–Xyloglucan–Lysozyme Dressing Seeded with Mesenchymal Stem Cells for Deep Second-Degree Burn Treatment
description Carolina Maria Costa de Oliveira Souza,1 Clayton Fernandes de Souza,2,3 Bassam Felipe Mogharbel,1 Ana Carolina Irioda,1 Celia Regina Cavichiolo Franco,4 Maria Rita Sierakowski,3 Katherine Athayde Teixeira de Carvalho1 1Stem Cell Research Laboratory, Cell Therapy and Biotechnology in Regenerative Medicine Department, Pequeno Príncipe Faculties and the Pelé Pequeno Príncipe Research Institute, Curitiba, Paraná, Brazil; 2Chemistry Undergraduate Program, School of Education and Humanities, Pontifícia Universidade Católica Do Paraná, Curitiba, Paraná, Brazil; 3BioPol, Chemistry Department, Federal University of Paraná, Curitiba, Paraná, Brazil; 4Molecular Biology Department, Federal University of Paraná, Curitiba, Paraná, BrazilCorrespondence: Katherine Athayde Teixeira de CarvalhoStem Cell Research Laboratory, Cell Therapy and Biotechnology in Regenerative Medicine Department, Pequeno Príncipe Faculties and the Pelé Pequeno Príncipe Research Institute, 1632 Avenida Silva Jardim, Box 80.240-020, Curitiba, Paraná, BrazilTel +55 41-3310-1034Email katherinecarv@gmail.comPurpose: In deep burns, wound contraction and hypertrophic scar formation can generate functional derangement and debilitation of the affected part. In order to improve the quality of healing in deep second-degree burns, we developed a new treatment in a preclinical model using nanostructured membranes seeded with mesenchymal stem cells (MSCs).Methods: Membranes were obtained by reconstitution of bacterial cellulose (reconstituted membrane [RM]) and produced by a dry-cast process, then RM was incorporated with 10% tamarind xyloglucan plus gellan gum 1:1 and 10% lysozyme (RMGT–LZ) and with 10% gellan gum and 10% lysozyme (RMG–LZ). Membrane hydrophobic/hydrophilic characteristics were investigated by static/dynamic contact-angle measurements. They were cultivated with MSCs, and cell adhesion, proliferation, and migration capacity was analyzed with MTT assays. Morphological and topographic characteristics were analyzed by scanning electron microscopy. MSC patterns in flow cytometry and differentiation into adipocytes and osteocytes were checked. In vivo assays used RMG–LZ and RMGT–LZ (with and without MSCs) in Rattus norvegicus rats submitted to burn protocol, and histological sections and collagen deposits were analyzed and immunocytochemistry assay performed.Results: In vitro results demonstrated carboxyl and amine groups made the membranes moderately hydrophobic and xyloglucan inclusion decreased wettability, favoring MSC adhesion, proliferation, and differentiation. In vivo, we obtained 40% and 60% reduction in acute/chronic inflammatory infiltrates, 96% decrease in injury area, increased vascular proliferation and collagen deposition, and complete epithelialization after 30 days. MSCs were detected in burned tissue, confirming they had homed and proliferated in vivo.Conclusion: Nanostructured cellulose–gellan–xyloglucan–lysozyme dressings, especially when seeded with MSCs, improved deep second-degree burn regeneration.Keywords: skin, cell-based therapy, transplantation, hydrocolloid dressing
format article
author Costa de Oliveira Souza CM
de Souza CF
Mogharbel BF
Irioda AC
Cavichiolo Franco CR
Sierakowski MR
Athayde Teixeira de Carvalho K
author_facet Costa de Oliveira Souza CM
de Souza CF
Mogharbel BF
Irioda AC
Cavichiolo Franco CR
Sierakowski MR
Athayde Teixeira de Carvalho K
author_sort Costa de Oliveira Souza CM
title Nanostructured Cellulose–Gellan–Xyloglucan–Lysozyme Dressing Seeded with Mesenchymal Stem Cells for Deep Second-Degree Burn Treatment
title_short Nanostructured Cellulose–Gellan–Xyloglucan–Lysozyme Dressing Seeded with Mesenchymal Stem Cells for Deep Second-Degree Burn Treatment
title_full Nanostructured Cellulose–Gellan–Xyloglucan–Lysozyme Dressing Seeded with Mesenchymal Stem Cells for Deep Second-Degree Burn Treatment
title_fullStr Nanostructured Cellulose–Gellan–Xyloglucan–Lysozyme Dressing Seeded with Mesenchymal Stem Cells for Deep Second-Degree Burn Treatment
title_full_unstemmed Nanostructured Cellulose–Gellan–Xyloglucan–Lysozyme Dressing Seeded with Mesenchymal Stem Cells for Deep Second-Degree Burn Treatment
title_sort nanostructured cellulose–gellan–xyloglucan–lysozyme dressing seeded with mesenchymal stem cells for deep second-degree burn treatment
publisher Dove Medical Press
publishDate 2021
url https://doaj.org/article/2e7d380f1767459f8eed5f1d866f2a72
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