Molecular characterization of the FCoV-like canine coronavirus HLJ-071 in China

Abstract Background According to the differences of antigen and genetic composition, canine coronavirus (CCoV) consists of two genotypes, CCoV-I and CCoV-II. Since 2004, CCoVs with point mutations or deletions of NSPs are contributing to the changes in tropism and virulence in dogs. Results In this...

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Autores principales:	Zhige Tian, Qing Pan, Miaomiao Zheng, Ying Deng, Peng Guo, Feng Cong, Xiaoliang Hu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Canine coronavirus Transcription-regulating sequence Recombination Cell tropism Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/2e89e997107e4bbc97733458f2cb4696
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spelling	oai:doaj.org-article:2e89e997107e4bbc97733458f2cb46962021-11-28T12:22:33ZMolecular characterization of the FCoV-like canine coronavirus HLJ-071 in China10.1186/s12917-021-03073-81746-6148https://doaj.org/article/2e89e997107e4bbc97733458f2cb46962021-11-01T00:00:00Zhttps://doi.org/10.1186/s12917-021-03073-8https://doaj.org/toc/1746-6148Abstract Background According to the differences of antigen and genetic composition, canine coronavirus (CCoV) consists of two genotypes, CCoV-I and CCoV-II. Since 2004, CCoVs with point mutations or deletions of NSPs are contributing to the changes in tropism and virulence in dogs. Results In this study, we isolated a CCoV, designated HLJ-071, from a dead 5-week-old female Welsh Corgi with severe diarrhea and vomit. Sequence analysis suggested that HLJ-071 bearing a complete ORF3abc compared with classic CCoV isolates (1-71, K378 and S378). In addition, a variable region was located between S gene and ORF 3a gene, in which a deletion with 104 nts for HLJ-071 when compared with classic CCoV strains 1-71, S378 and K378. Phylogenetic analysis based on the S gene and complete sequences showed that HLJ-071 was closely related to FCoV II. Recombination analysis suggested that HLJ-071 originated from the recombination of FCoV 79-1683, FCoV DF2 and CCoV A76. Finally, according to cell tropism experiments, it suggested that HLJ-071 could replicate in canine macrophages/monocytes cells. Conclusion The present study involved the isolation and genetic characterization of a variant CCoV strain and spike protein and ORF3abc of CCoV might play a key role in viral tropism, which could affect the replication in monocyte/macrophage cells. It will provide essential information for further understanding the evolution in China.Zhige TianQing PanMiaomiao ZhengYing DengPeng GuoFeng CongXiaoliang HuBMCarticleCanine coronavirusTranscription-regulating sequenceRecombinationCell tropismVeterinary medicineSF600-1100ENBMC Veterinary Research, Vol 17, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Canine coronavirus Transcription-regulating sequence Recombination Cell tropism Veterinary medicine SF600-1100
spellingShingle	Canine coronavirus Transcription-regulating sequence Recombination Cell tropism Veterinary medicine SF600-1100 Zhige Tian Qing Pan Miaomiao Zheng Ying Deng Peng Guo Feng Cong Xiaoliang Hu Molecular characterization of the FCoV-like canine coronavirus HLJ-071 in China
description	Abstract Background According to the differences of antigen and genetic composition, canine coronavirus (CCoV) consists of two genotypes, CCoV-I and CCoV-II. Since 2004, CCoVs with point mutations or deletions of NSPs are contributing to the changes in tropism and virulence in dogs. Results In this study, we isolated a CCoV, designated HLJ-071, from a dead 5-week-old female Welsh Corgi with severe diarrhea and vomit. Sequence analysis suggested that HLJ-071 bearing a complete ORF3abc compared with classic CCoV isolates (1-71, K378 and S378). In addition, a variable region was located between S gene and ORF 3a gene, in which a deletion with 104 nts for HLJ-071 when compared with classic CCoV strains 1-71, S378 and K378. Phylogenetic analysis based on the S gene and complete sequences showed that HLJ-071 was closely related to FCoV II. Recombination analysis suggested that HLJ-071 originated from the recombination of FCoV 79-1683, FCoV DF2 and CCoV A76. Finally, according to cell tropism experiments, it suggested that HLJ-071 could replicate in canine macrophages/monocytes cells. Conclusion The present study involved the isolation and genetic characterization of a variant CCoV strain and spike protein and ORF3abc of CCoV might play a key role in viral tropism, which could affect the replication in monocyte/macrophage cells. It will provide essential information for further understanding the evolution in China.
format	article
author	Zhige Tian Qing Pan Miaomiao Zheng Ying Deng Peng Guo Feng Cong Xiaoliang Hu
author_facet	Zhige Tian Qing Pan Miaomiao Zheng Ying Deng Peng Guo Feng Cong Xiaoliang Hu
author_sort	Zhige Tian
title	Molecular characterization of the FCoV-like canine coronavirus HLJ-071 in China
title_short	Molecular characterization of the FCoV-like canine coronavirus HLJ-071 in China
title_full	Molecular characterization of the FCoV-like canine coronavirus HLJ-071 in China
title_fullStr	Molecular characterization of the FCoV-like canine coronavirus HLJ-071 in China
title_full_unstemmed	Molecular characterization of the FCoV-like canine coronavirus HLJ-071 in China
title_sort	molecular characterization of the fcov-like canine coronavirus hlj-071 in china
publisher	BMC
publishDate	2021
url	https://doaj.org/article/2e89e997107e4bbc97733458f2cb4696
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Molecular characterization of the FCoV-like canine coronavirus HLJ-071 in China

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