Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells

Mesenchymal stem cells (MSCs) represent an attractive source within the field of tissue engineering. However, their harvesting often requires invasive medical procedures. Urine-derived stem cells (UDSCs) display similar properties to MSCs, and their obtention and further processing is non-invasive f...

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Autores principales:	Martina Culenova, Andreas Nicodemou, Zuzana Varchulova Novakova, Michaela Debreova, Veronika Smolinská, Sona Bernatova, Dana Ivanisova, Olga Novotna, Jaromir Vasicek, Ivan Varga, Stanislav Ziaran, Lubos Danisovic
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	urine-derived stem cells biological characteristics morphology differentiation capacity stemness cytokine profile Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2e8a757a5b554c4d8084f320a69cb257
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spelling	oai:doaj.org-article:2e8a757a5b554c4d8084f320a69cb2572021-11-25T17:57:18ZIsolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells10.3390/ijms2222125031422-00671661-6596https://doaj.org/article/2e8a757a5b554c4d8084f320a69cb2572021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12503https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Mesenchymal stem cells (MSCs) represent an attractive source within the field of tissue engineering. However, their harvesting often requires invasive medical procedures. Urine-derived stem cells (UDSCs) display similar properties to MSCs, and their obtention and further processing is non-invasive for the donors as well as low cost. Here, we offer a comprehensive analysis of their biological properties. The goal of this study was to analyze their morphology, stemness, differentiation potential and cytokine profile. We have successfully isolated UDSCs from 25 urine samples. First colonies emerged up to 9 days after the initial seeding. Cell doubling time was 45 ± 0.24 SD, and when seeded at the density of 100 cells/cm<sup>2</sup>, they formed 42 ± 6.5 SD colonies within 10 days. Morphological analyzes revealed that two different types of the cell populations have been present. The first type had a rice-grain shape and the second one was characterized by a polyhedral shape. In several cell cultures, dome-shaped cells were observed as well. All examined UDSCs expressed typical MSC-like surface markers, CD73, CD90 and CD105. Moreover, conditioned media from UDSCs were harvested, and cytokine profile has been evaluated showing a significantly higher secretory rate of IL-8, IL-6 and chemokines MCP-1 and GM-CSF. We have also successfully induced human UDSCs into chondrogenic, osteogenic and myogenic cell lineages. Our findings indicate that UDSCs might have immense potential in the regeneration of the damaged tissues.Martina CulenovaAndreas NicodemouZuzana Varchulova NovakovaMichaela DebreovaVeronika SmolinskáSona BernatovaDana IvanisovaOlga NovotnaJaromir VasicekIvan VargaStanislav ZiaranLubos DanisovicMDPI AGarticleurine-derived stem cellsbiological characteristicsmorphologydifferentiation capacitystemnesscytokine profileBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12503, p 12503 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	urine-derived stem cells biological characteristics morphology differentiation capacity stemness cytokine profile Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	urine-derived stem cells biological characteristics morphology differentiation capacity stemness cytokine profile Biology (General) QH301-705.5 Chemistry QD1-999 Martina Culenova Andreas Nicodemou Zuzana Varchulova Novakova Michaela Debreova Veronika Smolinská Sona Bernatova Dana Ivanisova Olga Novotna Jaromir Vasicek Ivan Varga Stanislav Ziaran Lubos Danisovic Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
description	Mesenchymal stem cells (MSCs) represent an attractive source within the field of tissue engineering. However, their harvesting often requires invasive medical procedures. Urine-derived stem cells (UDSCs) display similar properties to MSCs, and their obtention and further processing is non-invasive for the donors as well as low cost. Here, we offer a comprehensive analysis of their biological properties. The goal of this study was to analyze their morphology, stemness, differentiation potential and cytokine profile. We have successfully isolated UDSCs from 25 urine samples. First colonies emerged up to 9 days after the initial seeding. Cell doubling time was 45 ± 0.24 SD, and when seeded at the density of 100 cells/cm<sup>2</sup>, they formed 42 ± 6.5 SD colonies within 10 days. Morphological analyzes revealed that two different types of the cell populations have been present. The first type had a rice-grain shape and the second one was characterized by a polyhedral shape. In several cell cultures, dome-shaped cells were observed as well. All examined UDSCs expressed typical MSC-like surface markers, CD73, CD90 and CD105. Moreover, conditioned media from UDSCs were harvested, and cytokine profile has been evaluated showing a significantly higher secretory rate of IL-8, IL-6 and chemokines MCP-1 and GM-CSF. We have also successfully induced human UDSCs into chondrogenic, osteogenic and myogenic cell lineages. Our findings indicate that UDSCs might have immense potential in the regeneration of the damaged tissues.
format	article
author	Martina Culenova Andreas Nicodemou Zuzana Varchulova Novakova Michaela Debreova Veronika Smolinská Sona Bernatova Dana Ivanisova Olga Novotna Jaromir Vasicek Ivan Varga Stanislav Ziaran Lubos Danisovic
author_facet	Martina Culenova Andreas Nicodemou Zuzana Varchulova Novakova Michaela Debreova Veronika Smolinská Sona Bernatova Dana Ivanisova Olga Novotna Jaromir Vasicek Ivan Varga Stanislav Ziaran Lubos Danisovic
author_sort	Martina Culenova
title	Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
title_short	Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
title_full	Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
title_fullStr	Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
title_full_unstemmed	Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells
title_sort	isolation, culture and comprehensive characterization of biological properties of human urine-derived stem cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2e8a757a5b554c4d8084f320a69cb257
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Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells

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