Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate

The guanine nucleotide exchange factor SOS is a critical intermediary that transduces receptor tyrosine kinase stimulation into Ras activation. Here the authors develop a single molecule assay in which SOS is captured from raw lysates using Ras-functionalized membrane microarrays and uncover an auto...

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Autores principales:	Young Kwang Lee, Shalini T. Low-Nam, Jean K. Chung, Scott D. Hansen, Hiu Yue Monatrice Lam, Steven Alvarez, Jay T. Groves
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/2e94ef8418fb45d8a6f011beb41476a4
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spelling	oai:doaj.org-article:2e94ef8418fb45d8a6f011beb41476a42021-12-02T17:06:21ZMechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate10.1038/ncomms150612041-1723https://doaj.org/article/2e94ef8418fb45d8a6f011beb41476a42017-04-01T00:00:00Zhttps://doi.org/10.1038/ncomms15061https://doaj.org/toc/2041-1723The guanine nucleotide exchange factor SOS is a critical intermediary that transduces receptor tyrosine kinase stimulation into Ras activation. Here the authors develop a single molecule assay in which SOS is captured from raw lysates using Ras-functionalized membrane microarrays and uncover an autoinhibitory mechanism of SOS regulation.Young Kwang LeeShalini T. Low-NamJean K. ChungScott D. HansenHiu Yue Monatrice LamSteven AlvarezJay T. GrovesNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Young Kwang Lee Shalini T. Low-Nam Jean K. Chung Scott D. Hansen Hiu Yue Monatrice Lam Steven Alvarez Jay T. Groves Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate
description	The guanine nucleotide exchange factor SOS is a critical intermediary that transduces receptor tyrosine kinase stimulation into Ras activation. Here the authors develop a single molecule assay in which SOS is captured from raw lysates using Ras-functionalized membrane microarrays and uncover an autoinhibitory mechanism of SOS regulation.
format	article
author	Young Kwang Lee Shalini T. Low-Nam Jean K. Chung Scott D. Hansen Hiu Yue Monatrice Lam Steven Alvarez Jay T. Groves
author_facet	Young Kwang Lee Shalini T. Low-Nam Jean K. Chung Scott D. Hansen Hiu Yue Monatrice Lam Steven Alvarez Jay T. Groves
author_sort	Young Kwang Lee
title	Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate
title_short	Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate
title_full	Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate
title_fullStr	Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate
title_full_unstemmed	Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate
title_sort	mechanism of sos pr-domain autoinhibition revealed by single-molecule assays on native protein from lysate
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2e94ef8418fb45d8a6f011beb41476a4
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Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate

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