Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions

The monitoring of herpesvirus infection provides useful information when assessing marine mammals’ health. This paper shows the prevalence of herpesvirus infection (80.85%) in 47 cetaceans stranded on the coast of the Valencian Community, Spain. Of the 966 tissues evaluated, 121 tested positive when...

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Autores principales:	Ignacio Vargas-Castro, Mar Melero, José Luis Crespo-Picazo, María de los Ángeles Jiménez, Eva Sierra, Consuelo Rubio-Guerri, Manuel Arbelo, Antonio Fernández, Daniel García-Párraga, José Manuel Sánchez-Vizcaíno
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dolphin herpesvirus latency marine mammal coinfection morbillivirus Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2e96b52af2be4d8f84fe852967c69806
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spelling	oai:doaj.org-article:2e96b52af2be4d8f84fe852967c698062021-11-25T19:13:07ZSystematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions10.3390/v131121801999-4915https://doaj.org/article/2e96b52af2be4d8f84fe852967c698062021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2180https://doaj.org/toc/1999-4915The monitoring of herpesvirus infection provides useful information when assessing marine mammals’ health. This paper shows the prevalence of herpesvirus infection (80.85%) in 47 cetaceans stranded on the coast of the Valencian Community, Spain. Of the 966 tissues evaluated, 121 tested positive when employing nested-PCR (12.53%). The largest proportion of herpesvirus-positive tissue samples was in the reproductive system, nervous system, and tegument. Herpesvirus was more prevalent in females, juveniles, and calves. More than half the DNA PCR positive tissues contained herpesvirus RNA, indicating the presence of actively replicating virus. This RNA was most frequently found in neonates. Fourteen unique sequences were identified. Most amplified sequences belonged to the <i>Gammaherpesvirinae</i> subfamily, but a greater variation was found in <i>Alphaherpesvirinae</i> sequences. This is the first report of systematic herpesvirus DNA and RNA determination in free-ranging cetaceans. Nine (19.14%) were infected with cetacean morbillivirus and all of them (100%) were coinfected with herpesvirus. Lesions similar to those caused by herpesvirus in other species were observed, mainly in the skin, upper digestive tract, genitalia, and central nervous system. Other lesions were also attributable to concomitant etiologies or were nonspecific. It is necessary to investigate the possible role of herpesvirus infection in those cases.Ignacio Vargas-CastroMar MeleroJosé Luis Crespo-PicazoMaría de los Ángeles JiménezEva SierraConsuelo Rubio-GuerriManuel ArbeloAntonio FernándezDaniel García-PárragaJosé Manuel Sánchez-VizcaínoMDPI AGarticledolphinherpesviruslatencymarine mammalcoinfectionmorbillivirusMicrobiologyQR1-502ENViruses, Vol 13, Iss 2180, p 2180 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dolphin herpesvirus latency marine mammal coinfection morbillivirus Microbiology QR1-502
spellingShingle	dolphin herpesvirus latency marine mammal coinfection morbillivirus Microbiology QR1-502 Ignacio Vargas-Castro Mar Melero José Luis Crespo-Picazo María de los Ángeles Jiménez Eva Sierra Consuelo Rubio-Guerri Manuel Arbelo Antonio Fernández Daniel García-Párraga José Manuel Sánchez-Vizcaíno Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions
description	The monitoring of herpesvirus infection provides useful information when assessing marine mammals’ health. This paper shows the prevalence of herpesvirus infection (80.85%) in 47 cetaceans stranded on the coast of the Valencian Community, Spain. Of the 966 tissues evaluated, 121 tested positive when employing nested-PCR (12.53%). The largest proportion of herpesvirus-positive tissue samples was in the reproductive system, nervous system, and tegument. Herpesvirus was more prevalent in females, juveniles, and calves. More than half the DNA PCR positive tissues contained herpesvirus RNA, indicating the presence of actively replicating virus. This RNA was most frequently found in neonates. Fourteen unique sequences were identified. Most amplified sequences belonged to the <i>Gammaherpesvirinae</i> subfamily, but a greater variation was found in <i>Alphaherpesvirinae</i> sequences. This is the first report of systematic herpesvirus DNA and RNA determination in free-ranging cetaceans. Nine (19.14%) were infected with cetacean morbillivirus and all of them (100%) were coinfected with herpesvirus. Lesions similar to those caused by herpesvirus in other species were observed, mainly in the skin, upper digestive tract, genitalia, and central nervous system. Other lesions were also attributable to concomitant etiologies or were nonspecific. It is necessary to investigate the possible role of herpesvirus infection in those cases.
format	article
author	Ignacio Vargas-Castro Mar Melero José Luis Crespo-Picazo María de los Ángeles Jiménez Eva Sierra Consuelo Rubio-Guerri Manuel Arbelo Antonio Fernández Daniel García-Párraga José Manuel Sánchez-Vizcaíno
author_facet	Ignacio Vargas-Castro Mar Melero José Luis Crespo-Picazo María de los Ángeles Jiménez Eva Sierra Consuelo Rubio-Guerri Manuel Arbelo Antonio Fernández Daniel García-Párraga José Manuel Sánchez-Vizcaíno
author_sort	Ignacio Vargas-Castro
title	Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions
title_short	Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions
title_full	Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions
title_fullStr	Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions
title_full_unstemmed	Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions
title_sort	systematic determination of herpesvirus in free-ranging cetaceans stranded in the western mediterranean: tissue tropism and associated lesions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2e96b52af2be4d8f84fe852967c69806
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Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions

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