In vitro and in vivo human herpesvirus 8 infection of placenta.

Herpesvirus infection of placenta may be harmful in pregnancy leading to disorders in fetal growth, premature delivery, miscarriage, or major congenital abnormalities. Although a correlation between human herpesvirus 8 (HHV-8) infection and abortion or low birth weight in children has been suggested...

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Autores principales:	Mariantonietta Di Stefano, Maria Luisa Calabrò, Iole Maria Di Gangi, Santina Cantatore, Massimo Barbierato, Elisa Bergamo, Anfumbom Jude Kfutwah, Margherita Neri, Luigi Chieco-Bianchi, Pantaleo Greco, Loreto Gesualdo, Ahidjo Ayouba, Elisabeth Menu, Josè Ramòn Fiore
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2e9aac20262d4443a9e9b8931a696be0
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spelling	oai:doaj.org-article:2e9aac20262d4443a9e9b8931a696be02021-11-25T06:17:58ZIn vitro and in vivo human herpesvirus 8 infection of placenta.1932-620310.1371/journal.pone.0004073https://doaj.org/article/2e9aac20262d4443a9e9b8931a696be02008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19115001/?tool=EBIhttps://doaj.org/toc/1932-6203Herpesvirus infection of placenta may be harmful in pregnancy leading to disorders in fetal growth, premature delivery, miscarriage, or major congenital abnormalities. Although a correlation between human herpesvirus 8 (HHV-8) infection and abortion or low birth weight in children has been suggested, and rare cases of in utero or perinatal HHV-8 transmission have been documented, no direct evidence of HHV-8 infection of placenta has yet been reported. The aim of this study was to evaluate the in vitro and in vivo susceptibility of placental cells to HHV-8 infection. Short-term infection assays were performed on placental chorionic villi isolated from term placentae. Qualitative and quantitative HHV-8 detection were performed by PCR and real-time PCR, and HHV-8 proteins were analyzed by immunohistochemistry. Term placenta samples from HHV-8-seropositive women were analyzed for the presence of HHV-8 DNA and antigens. In vitro infected histocultures showed increasing amounts of HHV-8 DNA in tissues and supernatants; cyto- and syncitiotrophoblasts, as well as endothelial cells, expressed latent and lytic viral antigens. Increased apoptotic phenomena were visualized by the terminal deoxynucleotidyl transferase-mediated deoxyuridine nick end-labeling method in infected histocultures. Ex vivo, HHV-8 DNA and a latent viral antigen were detected in placenta samples from HHV-8-seropositive women. These findings demonstrate that HHV-8, like other human herpesviruses, may infect placental cells in vitro and in vivo, thus providing evidence that this phenomenon might influence vertical transmission and pregnancy outcome in HHV-8-infected women.Mariantonietta Di StefanoMaria Luisa CalabròIole Maria Di GangiSantina CantatoreMassimo BarbieratoElisa BergamoAnfumbom Jude KfutwahMargherita NeriLuigi Chieco-BianchiPantaleo GrecoLoreto GesualdoAhidjo AyoubaElisabeth MenuJosè Ramòn FiorePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 12, p e4073 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariantonietta Di Stefano Maria Luisa Calabrò Iole Maria Di Gangi Santina Cantatore Massimo Barbierato Elisa Bergamo Anfumbom Jude Kfutwah Margherita Neri Luigi Chieco-Bianchi Pantaleo Greco Loreto Gesualdo Ahidjo Ayouba Elisabeth Menu Josè Ramòn Fiore In vitro and in vivo human herpesvirus 8 infection of placenta.
description	Herpesvirus infection of placenta may be harmful in pregnancy leading to disorders in fetal growth, premature delivery, miscarriage, or major congenital abnormalities. Although a correlation between human herpesvirus 8 (HHV-8) infection and abortion or low birth weight in children has been suggested, and rare cases of in utero or perinatal HHV-8 transmission have been documented, no direct evidence of HHV-8 infection of placenta has yet been reported. The aim of this study was to evaluate the in vitro and in vivo susceptibility of placental cells to HHV-8 infection. Short-term infection assays were performed on placental chorionic villi isolated from term placentae. Qualitative and quantitative HHV-8 detection were performed by PCR and real-time PCR, and HHV-8 proteins were analyzed by immunohistochemistry. Term placenta samples from HHV-8-seropositive women were analyzed for the presence of HHV-8 DNA and antigens. In vitro infected histocultures showed increasing amounts of HHV-8 DNA in tissues and supernatants; cyto- and syncitiotrophoblasts, as well as endothelial cells, expressed latent and lytic viral antigens. Increased apoptotic phenomena were visualized by the terminal deoxynucleotidyl transferase-mediated deoxyuridine nick end-labeling method in infected histocultures. Ex vivo, HHV-8 DNA and a latent viral antigen were detected in placenta samples from HHV-8-seropositive women. These findings demonstrate that HHV-8, like other human herpesviruses, may infect placental cells in vitro and in vivo, thus providing evidence that this phenomenon might influence vertical transmission and pregnancy outcome in HHV-8-infected women.
format	article
author	Mariantonietta Di Stefano Maria Luisa Calabrò Iole Maria Di Gangi Santina Cantatore Massimo Barbierato Elisa Bergamo Anfumbom Jude Kfutwah Margherita Neri Luigi Chieco-Bianchi Pantaleo Greco Loreto Gesualdo Ahidjo Ayouba Elisabeth Menu Josè Ramòn Fiore
author_facet	Mariantonietta Di Stefano Maria Luisa Calabrò Iole Maria Di Gangi Santina Cantatore Massimo Barbierato Elisa Bergamo Anfumbom Jude Kfutwah Margherita Neri Luigi Chieco-Bianchi Pantaleo Greco Loreto Gesualdo Ahidjo Ayouba Elisabeth Menu Josè Ramòn Fiore
author_sort	Mariantonietta Di Stefano
title	In vitro and in vivo human herpesvirus 8 infection of placenta.
title_short	In vitro and in vivo human herpesvirus 8 infection of placenta.
title_full	In vitro and in vivo human herpesvirus 8 infection of placenta.
title_fullStr	In vitro and in vivo human herpesvirus 8 infection of placenta.
title_full_unstemmed	In vitro and in vivo human herpesvirus 8 infection of placenta.
title_sort	in vitro and in vivo human herpesvirus 8 infection of placenta.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/2e9aac20262d4443a9e9b8931a696be0
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In vitro and in vivo human herpesvirus 8 infection of placenta.

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