Meningitis Caused by the Live Varicella Vaccine Virus: Metagenomic Next Generation Sequencing, Immunology Exome Sequencing and Cytokine Multiplex Profiling
Varicella vaccine meningitis is an uncommon delayed adverse event of vaccination. Varicella vaccine meningitis has been diagnosed in 12 children, of whom 3 were immunocompromised. We now report two additional cases of vaccine meningitis in twice-immunized immunocompetent children and we perform furt...
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2021
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oai:doaj.org-article:2e9acd197dfb4512959c5818668614e02021-11-25T19:14:11ZMeningitis Caused by the Live Varicella Vaccine Virus: Metagenomic Next Generation Sequencing, Immunology Exome Sequencing and Cytokine Multiplex Profiling10.3390/v131122861999-4915https://doaj.org/article/2e9acd197dfb4512959c5818668614e02021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2286https://doaj.org/toc/1999-4915Varicella vaccine meningitis is an uncommon delayed adverse event of vaccination. Varicella vaccine meningitis has been diagnosed in 12 children, of whom 3 were immunocompromised. We now report two additional cases of vaccine meningitis in twice-immunized immunocompetent children and we perform further testing on a prior third case. We used three methods to diagnose or investigate cases of varicella vaccine meningitis, none of which have been used previously on this disease. These include metagenomic next-generation sequencing and cytokine multiplex profiling of cerebrospinal fluid and immunology exome analysis of white blood cells. In one new case, the diagnosis was confirmed by metagenomic next-generation sequencing of cerebrospinal fluid. Both varicella vaccine virus and human herpesvirus 7 DNA were detected. We performed cytokine multiplex profiling on the cerebrospinal fluid of two cases and found ten elevated biomarkers: interferon gamma, interleukins IL-1RA, IL-6, IL-8, IL-10, IL-17F, chemokines CXCL-9, CXCL-10, CCL-2, and G-CSF. In a second new case, we performed immunology exome sequencing on a panel of 356 genes, but no errors were found. After a review of all 14 cases, we concluded that (i) there is no common explanation for this adverse event, but (ii) ingestion of an oral corticosteroid burst 3–4 weeks before onset of vaccine meningitis may be a risk factor in some cases.Prashanth S. RamachandranMichael R. WilsonGaud CathoGeraldine Blanchard-RohnerNicoline SchiessRandall J. CohrsDavid BoutolleauSonia BurrelTetsushi YoshikawaAnne WapniarskiEthan H. HeuselJohn E. CarpenterWallen JacksonBradley A. FordCharles GroseMDPI AGarticlevaricella-zoster virusOka strainhuman herpesvirus 6human herpesvirus 7corticosteroidsserious adverse eventMicrobiologyQR1-502ENViruses, Vol 13, Iss 2286, p 2286 (2021) |
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varicella-zoster virus Oka strain human herpesvirus 6 human herpesvirus 7 corticosteroids serious adverse event Microbiology QR1-502 |
| spellingShingle |
varicella-zoster virus Oka strain human herpesvirus 6 human herpesvirus 7 corticosteroids serious adverse event Microbiology QR1-502 Prashanth S. Ramachandran Michael R. Wilson Gaud Catho Geraldine Blanchard-Rohner Nicoline Schiess Randall J. Cohrs David Boutolleau Sonia Burrel Tetsushi Yoshikawa Anne Wapniarski Ethan H. Heusel John E. Carpenter Wallen Jackson Bradley A. Ford Charles Grose Meningitis Caused by the Live Varicella Vaccine Virus: Metagenomic Next Generation Sequencing, Immunology Exome Sequencing and Cytokine Multiplex Profiling |
| description |
Varicella vaccine meningitis is an uncommon delayed adverse event of vaccination. Varicella vaccine meningitis has been diagnosed in 12 children, of whom 3 were immunocompromised. We now report two additional cases of vaccine meningitis in twice-immunized immunocompetent children and we perform further testing on a prior third case. We used three methods to diagnose or investigate cases of varicella vaccine meningitis, none of which have been used previously on this disease. These include metagenomic next-generation sequencing and cytokine multiplex profiling of cerebrospinal fluid and immunology exome analysis of white blood cells. In one new case, the diagnosis was confirmed by metagenomic next-generation sequencing of cerebrospinal fluid. Both varicella vaccine virus and human herpesvirus 7 DNA were detected. We performed cytokine multiplex profiling on the cerebrospinal fluid of two cases and found ten elevated biomarkers: interferon gamma, interleukins IL-1RA, IL-6, IL-8, IL-10, IL-17F, chemokines CXCL-9, CXCL-10, CCL-2, and G-CSF. In a second new case, we performed immunology exome sequencing on a panel of 356 genes, but no errors were found. After a review of all 14 cases, we concluded that (i) there is no common explanation for this adverse event, but (ii) ingestion of an oral corticosteroid burst 3–4 weeks before onset of vaccine meningitis may be a risk factor in some cases. |
| format |
article |
| author |
Prashanth S. Ramachandran Michael R. Wilson Gaud Catho Geraldine Blanchard-Rohner Nicoline Schiess Randall J. Cohrs David Boutolleau Sonia Burrel Tetsushi Yoshikawa Anne Wapniarski Ethan H. Heusel John E. Carpenter Wallen Jackson Bradley A. Ford Charles Grose |
| author_facet |
Prashanth S. Ramachandran Michael R. Wilson Gaud Catho Geraldine Blanchard-Rohner Nicoline Schiess Randall J. Cohrs David Boutolleau Sonia Burrel Tetsushi Yoshikawa Anne Wapniarski Ethan H. Heusel John E. Carpenter Wallen Jackson Bradley A. Ford Charles Grose |
| author_sort |
Prashanth S. Ramachandran |
| title |
Meningitis Caused by the Live Varicella Vaccine Virus: Metagenomic Next Generation Sequencing, Immunology Exome Sequencing and Cytokine Multiplex Profiling |
| title_short |
Meningitis Caused by the Live Varicella Vaccine Virus: Metagenomic Next Generation Sequencing, Immunology Exome Sequencing and Cytokine Multiplex Profiling |
| title_full |
Meningitis Caused by the Live Varicella Vaccine Virus: Metagenomic Next Generation Sequencing, Immunology Exome Sequencing and Cytokine Multiplex Profiling |
| title_fullStr |
Meningitis Caused by the Live Varicella Vaccine Virus: Metagenomic Next Generation Sequencing, Immunology Exome Sequencing and Cytokine Multiplex Profiling |
| title_full_unstemmed |
Meningitis Caused by the Live Varicella Vaccine Virus: Metagenomic Next Generation Sequencing, Immunology Exome Sequencing and Cytokine Multiplex Profiling |
| title_sort |
meningitis caused by the live varicella vaccine virus: metagenomic next generation sequencing, immunology exome sequencing and cytokine multiplex profiling |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/2e9acd197dfb4512959c5818668614e0 |
| work_keys_str_mv |
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