Osteogenic differentiation capacity of human skeletal muscle-derived progenitor cells.

Heterotopic ossification (HO) is defined as the formation of ectopic bone in soft tissue outside the skeletal tissue. HO is thought to result from aberrant differentiation of osteogenic progenitors within skeletal muscle. However, the precise origin of HO is still unclear. Skeletal muscle contains t...

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Autores principales:	Teruyo Oishi, Akiyoshi Uezumi, Arihiko Kanaji, Naoki Yamamoto, Asami Yamaguchi, Harumoto Yamada, Kunihiro Tsuchida
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ea087da67bb4b90801170583efd809b
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spelling	oai:doaj.org-article:2ea087da67bb4b90801170583efd809b2021-11-18T07:57:25ZOsteogenic differentiation capacity of human skeletal muscle-derived progenitor cells.1932-620310.1371/journal.pone.0056641https://doaj.org/article/2ea087da67bb4b90801170583efd809b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23457598/?tool=EBIhttps://doaj.org/toc/1932-6203Heterotopic ossification (HO) is defined as the formation of ectopic bone in soft tissue outside the skeletal tissue. HO is thought to result from aberrant differentiation of osteogenic progenitors within skeletal muscle. However, the precise origin of HO is still unclear. Skeletal muscle contains two kinds of progenitor cells, myogenic progenitors and mesenchymal progenitors. Myogenic and mesenchymal progenitors in human skeletal muscle can be identified as CD56(+) and PDGFRα(+) cells, respectively. The purpose of this study was to investigate the osteogenic differentiation potential of human skeletal muscle-derived progenitors. Both CD56(+) cells and PDGFRα(+) cells showed comparable osteogenic differentiation potential in vitro. However, in an in vivo ectopic bone formation model, PDGFRα(+) cells formed bone-like tissue and showed successful engraftment, while CD56(+) cells did not form bone-like tissue and did not adapt to an osteogenic environment. Immunohistological analysis of human HO sample revealed that many PDGFRα(+) cells were localized in proximity to ectopic bone formed in skeletal muscle. MicroRNAs (miRNAs) are known to regulate many biological processes including osteogenic differentiation. We investigated the participation of miRNAs in the osteogenic differentiation of PDGFRα(+) cells by using microarray. We identified miRNAs that had not been known to be involved in osteogenesis but showed dramatic changes during osteogenic differentiation of PDGFRα(+) cells. Upregulation of miR-146b-5p and -424 and downregulation of miR-7 during osteogenic differentiation of PDGFRα(+) cells were confirmed by quantitative real-time RT-PCR. Inhibition of upregulated miRNAs, miR-146b-5p and -424, resulted in the suppression of osteocyte maturation, suggesting that these two miRNAs have the positive role in the osteogenesis of PDGFRα(+) cells. Our results suggest that PDGFRα(+) cells may be the major source of HO and that the newly identified miRNAs may regulate osteogenic differentiation process of PDGFRα(+) cells.Teruyo OishiAkiyoshi UezumiArihiko KanajiNaoki YamamotoAsami YamaguchiHarumoto YamadaKunihiro TsuchidaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56641 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Teruyo Oishi Akiyoshi Uezumi Arihiko Kanaji Naoki Yamamoto Asami Yamaguchi Harumoto Yamada Kunihiro Tsuchida Osteogenic differentiation capacity of human skeletal muscle-derived progenitor cells.
description	Heterotopic ossification (HO) is defined as the formation of ectopic bone in soft tissue outside the skeletal tissue. HO is thought to result from aberrant differentiation of osteogenic progenitors within skeletal muscle. However, the precise origin of HO is still unclear. Skeletal muscle contains two kinds of progenitor cells, myogenic progenitors and mesenchymal progenitors. Myogenic and mesenchymal progenitors in human skeletal muscle can be identified as CD56(+) and PDGFRα(+) cells, respectively. The purpose of this study was to investigate the osteogenic differentiation potential of human skeletal muscle-derived progenitors. Both CD56(+) cells and PDGFRα(+) cells showed comparable osteogenic differentiation potential in vitro. However, in an in vivo ectopic bone formation model, PDGFRα(+) cells formed bone-like tissue and showed successful engraftment, while CD56(+) cells did not form bone-like tissue and did not adapt to an osteogenic environment. Immunohistological analysis of human HO sample revealed that many PDGFRα(+) cells were localized in proximity to ectopic bone formed in skeletal muscle. MicroRNAs (miRNAs) are known to regulate many biological processes including osteogenic differentiation. We investigated the participation of miRNAs in the osteogenic differentiation of PDGFRα(+) cells by using microarray. We identified miRNAs that had not been known to be involved in osteogenesis but showed dramatic changes during osteogenic differentiation of PDGFRα(+) cells. Upregulation of miR-146b-5p and -424 and downregulation of miR-7 during osteogenic differentiation of PDGFRα(+) cells were confirmed by quantitative real-time RT-PCR. Inhibition of upregulated miRNAs, miR-146b-5p and -424, resulted in the suppression of osteocyte maturation, suggesting that these two miRNAs have the positive role in the osteogenesis of PDGFRα(+) cells. Our results suggest that PDGFRα(+) cells may be the major source of HO and that the newly identified miRNAs may regulate osteogenic differentiation process of PDGFRα(+) cells.
format	article
author	Teruyo Oishi Akiyoshi Uezumi Arihiko Kanaji Naoki Yamamoto Asami Yamaguchi Harumoto Yamada Kunihiro Tsuchida
author_facet	Teruyo Oishi Akiyoshi Uezumi Arihiko Kanaji Naoki Yamamoto Asami Yamaguchi Harumoto Yamada Kunihiro Tsuchida
author_sort	Teruyo Oishi
title	Osteogenic differentiation capacity of human skeletal muscle-derived progenitor cells.
title_short	Osteogenic differentiation capacity of human skeletal muscle-derived progenitor cells.
title_full	Osteogenic differentiation capacity of human skeletal muscle-derived progenitor cells.
title_fullStr	Osteogenic differentiation capacity of human skeletal muscle-derived progenitor cells.
title_full_unstemmed	Osteogenic differentiation capacity of human skeletal muscle-derived progenitor cells.
title_sort	osteogenic differentiation capacity of human skeletal muscle-derived progenitor cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/2ea087da67bb4b90801170583efd809b
work_keys_str_mv	AT teruyooishi osteogenicdifferentiationcapacityofhumanskeletalmusclederivedprogenitorcells AT akiyoshiuezumi osteogenicdifferentiationcapacityofhumanskeletalmusclederivedprogenitorcells AT arihikokanaji osteogenicdifferentiationcapacityofhumanskeletalmusclederivedprogenitorcells AT naokiyamamoto osteogenicdifferentiationcapacityofhumanskeletalmusclederivedprogenitorcells AT asamiyamaguchi osteogenicdifferentiationcapacityofhumanskeletalmusclederivedprogenitorcells AT harumotoyamada osteogenicdifferentiationcapacityofhumanskeletalmusclederivedprogenitorcells AT kunihirotsuchida osteogenicdifferentiationcapacityofhumanskeletalmusclederivedprogenitorcells
_version_	1718422719940263936

Osteogenic differentiation capacity of human skeletal muscle-derived progenitor cells.

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