Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis

Hashimoto’s thyroiditis (HT) is an autoimmune disease, and its incidence continues to rise. Although scientists have studied this disease for many years and discovered the potential effects of various proteins in it, the specific pathogenesis is still not fully comprehended. To understand HT and tra...

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Autores principales: Hui Xiao, Jianqing Liang, Sunqiang Liu, Qiongyue Zhang, Famin Xie, Xingyu Kong, Shanshan Guo, Ruwen Wang, Rong Fu, Zhiqi Ye, Yun Li, Shuang Zhang, Li Zhang, Keneilwe Kenny Kaudimba, Ru Wang, Xingxing Kong, Bing Zhao, Xuqin Zheng, Tiemin Liu
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Publicado: Frontiers Media S.A. 2021
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Acceso en línea:https://doaj.org/article/2ea182fac0ec4c3791d263a2cbf779b8
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spelling oai:doaj.org-article:2ea182fac0ec4c3791d263a2cbf779b82021-12-02T10:35:49ZProteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis1664-322410.3389/fimmu.2021.784975https://doaj.org/article/2ea182fac0ec4c3791d263a2cbf779b82021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.784975/fullhttps://doaj.org/toc/1664-3224Hashimoto’s thyroiditis (HT) is an autoimmune disease, and its incidence continues to rise. Although scientists have studied this disease for many years and discovered the potential effects of various proteins in it, the specific pathogenesis is still not fully comprehended. To understand HT and translate this knowledge to clinical applications, we took the mass spectrometric analysis on thyroid tissue fine-needle puncture from HT patients and healthy people in an attempt to make a further understanding of the pathogenesis of HT. A total of 44 proteins with differential expression were identified in HT patients, and these proteins play vital roles in cell adhesion, cell metabolism, and thyroxine synthesis. Combining patient clinical trial sample information, we further compared the transient changes of gene expression regulation in HT and papillary thyroid carcinoma (PTC) samples. More importantly, we developed patient-derived HT and PTC organoids as a promising new preclinical model to verify these potential markers. Our data revealed a marked characteristic of HT organoid in upregulating chemokines that include C-C motif chemokine ligand (CCL) 2 and CCL3, which play a key role in the pathogenesis of HT. Overall, our research has enriched everyone’s understanding of the pathogenesis of HT and provides a certain reference for the treatment of the disease.Hui XiaoJianqing LiangSunqiang LiuQiongyue ZhangQiongyue ZhangFamin XieXingyu KongShanshan GuoRuwen WangRuwen WangRong FuZhiqi YeYun LiShuang ZhangShuang ZhangLi ZhangKeneilwe Kenny KaudimbaKeneilwe Kenny KaudimbaRu WangRu WangXingxing KongXingxing KongBing ZhaoXuqin ZhengTiemin LiuTiemin LiuTiemin LiuFrontiers Media S.A.articleHashimoto’s thyroiditisautoimmune diseasesproteomicsorganoidpathogenesisImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Hashimoto’s thyroiditis
autoimmune diseases
proteomics
organoid
pathogenesis
Immunologic diseases. Allergy
RC581-607
spellingShingle Hashimoto’s thyroiditis
autoimmune diseases
proteomics
organoid
pathogenesis
Immunologic diseases. Allergy
RC581-607
Hui Xiao
Jianqing Liang
Sunqiang Liu
Qiongyue Zhang
Qiongyue Zhang
Famin Xie
Xingyu Kong
Shanshan Guo
Ruwen Wang
Ruwen Wang
Rong Fu
Zhiqi Ye
Yun Li
Shuang Zhang
Shuang Zhang
Li Zhang
Keneilwe Kenny Kaudimba
Keneilwe Kenny Kaudimba
Ru Wang
Ru Wang
Xingxing Kong
Xingxing Kong
Bing Zhao
Xuqin Zheng
Tiemin Liu
Tiemin Liu
Tiemin Liu
Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
description Hashimoto’s thyroiditis (HT) is an autoimmune disease, and its incidence continues to rise. Although scientists have studied this disease for many years and discovered the potential effects of various proteins in it, the specific pathogenesis is still not fully comprehended. To understand HT and translate this knowledge to clinical applications, we took the mass spectrometric analysis on thyroid tissue fine-needle puncture from HT patients and healthy people in an attempt to make a further understanding of the pathogenesis of HT. A total of 44 proteins with differential expression were identified in HT patients, and these proteins play vital roles in cell adhesion, cell metabolism, and thyroxine synthesis. Combining patient clinical trial sample information, we further compared the transient changes of gene expression regulation in HT and papillary thyroid carcinoma (PTC) samples. More importantly, we developed patient-derived HT and PTC organoids as a promising new preclinical model to verify these potential markers. Our data revealed a marked characteristic of HT organoid in upregulating chemokines that include C-C motif chemokine ligand (CCL) 2 and CCL3, which play a key role in the pathogenesis of HT. Overall, our research has enriched everyone’s understanding of the pathogenesis of HT and provides a certain reference for the treatment of the disease.
format article
author Hui Xiao
Jianqing Liang
Sunqiang Liu
Qiongyue Zhang
Qiongyue Zhang
Famin Xie
Xingyu Kong
Shanshan Guo
Ruwen Wang
Ruwen Wang
Rong Fu
Zhiqi Ye
Yun Li
Shuang Zhang
Shuang Zhang
Li Zhang
Keneilwe Kenny Kaudimba
Keneilwe Kenny Kaudimba
Ru Wang
Ru Wang
Xingxing Kong
Xingxing Kong
Bing Zhao
Xuqin Zheng
Tiemin Liu
Tiemin Liu
Tiemin Liu
author_facet Hui Xiao
Jianqing Liang
Sunqiang Liu
Qiongyue Zhang
Qiongyue Zhang
Famin Xie
Xingyu Kong
Shanshan Guo
Ruwen Wang
Ruwen Wang
Rong Fu
Zhiqi Ye
Yun Li
Shuang Zhang
Shuang Zhang
Li Zhang
Keneilwe Kenny Kaudimba
Keneilwe Kenny Kaudimba
Ru Wang
Ru Wang
Xingxing Kong
Xingxing Kong
Bing Zhao
Xuqin Zheng
Tiemin Liu
Tiemin Liu
Tiemin Liu
author_sort Hui Xiao
title Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
title_short Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
title_full Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
title_fullStr Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
title_full_unstemmed Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
title_sort proteomics and organoid culture reveal the underlying pathogenesis of hashimoto’s thyroiditis
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/2ea182fac0ec4c3791d263a2cbf779b8
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