Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis
Hashimoto’s thyroiditis (HT) is an autoimmune disease, and its incidence continues to rise. Although scientists have studied this disease for many years and discovered the potential effects of various proteins in it, the specific pathogenesis is still not fully comprehended. To understand HT and tra...
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2021
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oai:doaj.org-article:2ea182fac0ec4c3791d263a2cbf779b82021-12-02T10:35:49ZProteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis1664-322410.3389/fimmu.2021.784975https://doaj.org/article/2ea182fac0ec4c3791d263a2cbf779b82021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.784975/fullhttps://doaj.org/toc/1664-3224Hashimoto’s thyroiditis (HT) is an autoimmune disease, and its incidence continues to rise. Although scientists have studied this disease for many years and discovered the potential effects of various proteins in it, the specific pathogenesis is still not fully comprehended. To understand HT and translate this knowledge to clinical applications, we took the mass spectrometric analysis on thyroid tissue fine-needle puncture from HT patients and healthy people in an attempt to make a further understanding of the pathogenesis of HT. A total of 44 proteins with differential expression were identified in HT patients, and these proteins play vital roles in cell adhesion, cell metabolism, and thyroxine synthesis. Combining patient clinical trial sample information, we further compared the transient changes of gene expression regulation in HT and papillary thyroid carcinoma (PTC) samples. More importantly, we developed patient-derived HT and PTC organoids as a promising new preclinical model to verify these potential markers. Our data revealed a marked characteristic of HT organoid in upregulating chemokines that include C-C motif chemokine ligand (CCL) 2 and CCL3, which play a key role in the pathogenesis of HT. Overall, our research has enriched everyone’s understanding of the pathogenesis of HT and provides a certain reference for the treatment of the disease.Hui XiaoJianqing LiangSunqiang LiuQiongyue ZhangQiongyue ZhangFamin XieXingyu KongShanshan GuoRuwen WangRuwen WangRong FuZhiqi YeYun LiShuang ZhangShuang ZhangLi ZhangKeneilwe Kenny KaudimbaKeneilwe Kenny KaudimbaRu WangRu WangXingxing KongXingxing KongBing ZhaoXuqin ZhengTiemin LiuTiemin LiuTiemin LiuFrontiers Media S.A.articleHashimoto’s thyroiditisautoimmune diseasesproteomicsorganoidpathogenesisImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Hashimoto’s thyroiditis autoimmune diseases proteomics organoid pathogenesis Immunologic diseases. Allergy RC581-607 |
spellingShingle |
Hashimoto’s thyroiditis autoimmune diseases proteomics organoid pathogenesis Immunologic diseases. Allergy RC581-607 Hui Xiao Jianqing Liang Sunqiang Liu Qiongyue Zhang Qiongyue Zhang Famin Xie Xingyu Kong Shanshan Guo Ruwen Wang Ruwen Wang Rong Fu Zhiqi Ye Yun Li Shuang Zhang Shuang Zhang Li Zhang Keneilwe Kenny Kaudimba Keneilwe Kenny Kaudimba Ru Wang Ru Wang Xingxing Kong Xingxing Kong Bing Zhao Xuqin Zheng Tiemin Liu Tiemin Liu Tiemin Liu Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis |
description |
Hashimoto’s thyroiditis (HT) is an autoimmune disease, and its incidence continues to rise. Although scientists have studied this disease for many years and discovered the potential effects of various proteins in it, the specific pathogenesis is still not fully comprehended. To understand HT and translate this knowledge to clinical applications, we took the mass spectrometric analysis on thyroid tissue fine-needle puncture from HT patients and healthy people in an attempt to make a further understanding of the pathogenesis of HT. A total of 44 proteins with differential expression were identified in HT patients, and these proteins play vital roles in cell adhesion, cell metabolism, and thyroxine synthesis. Combining patient clinical trial sample information, we further compared the transient changes of gene expression regulation in HT and papillary thyroid carcinoma (PTC) samples. More importantly, we developed patient-derived HT and PTC organoids as a promising new preclinical model to verify these potential markers. Our data revealed a marked characteristic of HT organoid in upregulating chemokines that include C-C motif chemokine ligand (CCL) 2 and CCL3, which play a key role in the pathogenesis of HT. Overall, our research has enriched everyone’s understanding of the pathogenesis of HT and provides a certain reference for the treatment of the disease. |
format |
article |
author |
Hui Xiao Jianqing Liang Sunqiang Liu Qiongyue Zhang Qiongyue Zhang Famin Xie Xingyu Kong Shanshan Guo Ruwen Wang Ruwen Wang Rong Fu Zhiqi Ye Yun Li Shuang Zhang Shuang Zhang Li Zhang Keneilwe Kenny Kaudimba Keneilwe Kenny Kaudimba Ru Wang Ru Wang Xingxing Kong Xingxing Kong Bing Zhao Xuqin Zheng Tiemin Liu Tiemin Liu Tiemin Liu |
author_facet |
Hui Xiao Jianqing Liang Sunqiang Liu Qiongyue Zhang Qiongyue Zhang Famin Xie Xingyu Kong Shanshan Guo Ruwen Wang Ruwen Wang Rong Fu Zhiqi Ye Yun Li Shuang Zhang Shuang Zhang Li Zhang Keneilwe Kenny Kaudimba Keneilwe Kenny Kaudimba Ru Wang Ru Wang Xingxing Kong Xingxing Kong Bing Zhao Xuqin Zheng Tiemin Liu Tiemin Liu Tiemin Liu |
author_sort |
Hui Xiao |
title |
Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis |
title_short |
Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis |
title_full |
Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis |
title_fullStr |
Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis |
title_full_unstemmed |
Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto’s Thyroiditis |
title_sort |
proteomics and organoid culture reveal the underlying pathogenesis of hashimoto’s thyroiditis |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doaj.org/article/2ea182fac0ec4c3791d263a2cbf779b8 |
work_keys_str_mv |
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