A general approach to engineer positive-going eFRET voltage indicators

Genetically encoded voltage indicators (GEVIs) allow visualisation of fast action potentials in neurons but most are bright at rest and dimmer during an action potential. Here, the authors engineer electrochromic FRET GEVIs with fast, bright and positive-going fluorescence signals for in vivo imagin...

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Autores principales:	Ahmed S. Abdelfattah, Rosario Valenti, Jihong Zheng, Allan Wong, GENIE Project Team, Kaspar Podgorski, Minoru Koyama, Douglas S. Kim, Eric R. Schreiter
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/2ea2ff2c1bd44acdb9e04c2ab44cdf71
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spelling	oai:doaj.org-article:2ea2ff2c1bd44acdb9e04c2ab44cdf712021-12-02T15:39:43ZA general approach to engineer positive-going eFRET voltage indicators10.1038/s41467-020-17322-12041-1723https://doaj.org/article/2ea2ff2c1bd44acdb9e04c2ab44cdf712020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17322-1https://doaj.org/toc/2041-1723Genetically encoded voltage indicators (GEVIs) allow visualisation of fast action potentials in neurons but most are bright at rest and dimmer during an action potential. Here, the authors engineer electrochromic FRET GEVIs with fast, bright and positive-going fluorescence signals for in vivo imaging.Ahmed S. AbdelfattahRosario ValentiJihong ZhengAllan WongGENIE Project TeamKaspar PodgorskiMinoru KoyamaDouglas S. KimEric R. SchreiterNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ahmed S. Abdelfattah Rosario Valenti Jihong Zheng Allan Wong GENIE Project Team Kaspar Podgorski Minoru Koyama Douglas S. Kim Eric R. Schreiter A general approach to engineer positive-going eFRET voltage indicators
description	Genetically encoded voltage indicators (GEVIs) allow visualisation of fast action potentials in neurons but most are bright at rest and dimmer during an action potential. Here, the authors engineer electrochromic FRET GEVIs with fast, bright and positive-going fluorescence signals for in vivo imaging.
format	article
author	Ahmed S. Abdelfattah Rosario Valenti Jihong Zheng Allan Wong GENIE Project Team Kaspar Podgorski Minoru Koyama Douglas S. Kim Eric R. Schreiter
author_facet	Ahmed S. Abdelfattah Rosario Valenti Jihong Zheng Allan Wong GENIE Project Team Kaspar Podgorski Minoru Koyama Douglas S. Kim Eric R. Schreiter
author_sort	Ahmed S. Abdelfattah
title	A general approach to engineer positive-going eFRET voltage indicators
title_short	A general approach to engineer positive-going eFRET voltage indicators
title_full	A general approach to engineer positive-going eFRET voltage indicators
title_fullStr	A general approach to engineer positive-going eFRET voltage indicators
title_full_unstemmed	A general approach to engineer positive-going eFRET voltage indicators
title_sort	general approach to engineer positive-going efret voltage indicators
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2ea2ff2c1bd44acdb9e04c2ab44cdf71
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A general approach to engineer positive-going eFRET voltage indicators

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