Therapeutic effects of hMAPC and hMSC transplantation after stroke in mice.

Stroke represents an attractive target for stem cell therapy. Although different types of cells have been employed in animal models, a direct comparison between cell sources has not been performed. The aim of our study was to assess the effect of human multipotent adult progenitor cells (hMAPCs) and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Silvia Mora-Lee, Ma Salomé Sirerol-Piquer, María Gutiérrez-Pérez, Ulises Gomez-Pinedo, Valerie D Roobrouck, Tania López, Mayte Casado-Nieto, Gloria Abizanda, Maria Teresa Rabena, Catherine Verfaille, Felipe Prósper, Jose Manuel García-Verdugo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ea8361a7bc74b268f115131262f70dc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2ea8361a7bc74b268f115131262f70dc
record_format	dspace
spelling	oai:doaj.org-article:2ea8361a7bc74b268f115131262f70dc2021-11-18T07:06:55ZTherapeutic effects of hMAPC and hMSC transplantation after stroke in mice.1932-620310.1371/journal.pone.0043683https://doaj.org/article/2ea8361a7bc74b268f115131262f70dc2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952736/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Stroke represents an attractive target for stem cell therapy. Although different types of cells have been employed in animal models, a direct comparison between cell sources has not been performed. The aim of our study was to assess the effect of human multipotent adult progenitor cells (hMAPCs) and human mesenchymal stem cells (hMSCs) on endogenous neurogenesis, angiogenesis and inflammation following stroke. BALB/Ca-RAG 2(-/-) γC(-/-) mice subjected to FeCl(3) thrombosis mediated stroke were intracranially injected with 2 × 10(5) hMAPCs or hMSCs 2 days after stroke and followed for up to 28 days. We could not detect long-term engraftment of either cell population. However, in comparison with PBS-treated animals, hMSC and hMAPC grafted animals demonstrated significantly decreased loss of brain tissue. This was associated with increased angiogenesis, diminished inflammation and a glial-scar inhibitory effect. Moreover, enhanced proliferation of cells in the subventricular zone (SVZ) and survival of newly generated neuroblasts was observed. Interestingly, these neuroprotective effects were more pronounced in the group of animals treated with hMAPCs in comparison with hMSCs. Our results establish cell therapy with hMAPCs and hMSCs as a promising strategy for the treatment of stroke.Silvia Mora-LeeMa Salomé Sirerol-PiquerMaría Gutiérrez-PérezUlises Gomez-PinedoValerie D RoobrouckTania LópezMayte Casado-NietoGloria AbizandaMaria Teresa RabenaCatherine VerfailleFelipe PrósperJose Manuel García-VerdugoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43683 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Silvia Mora-Lee Ma Salomé Sirerol-Piquer María Gutiérrez-Pérez Ulises Gomez-Pinedo Valerie D Roobrouck Tania López Mayte Casado-Nieto Gloria Abizanda Maria Teresa Rabena Catherine Verfaille Felipe Prósper Jose Manuel García-Verdugo Therapeutic effects of hMAPC and hMSC transplantation after stroke in mice.
description	Stroke represents an attractive target for stem cell therapy. Although different types of cells have been employed in animal models, a direct comparison between cell sources has not been performed. The aim of our study was to assess the effect of human multipotent adult progenitor cells (hMAPCs) and human mesenchymal stem cells (hMSCs) on endogenous neurogenesis, angiogenesis and inflammation following stroke. BALB/Ca-RAG 2(-/-) γC(-/-) mice subjected to FeCl(3) thrombosis mediated stroke were intracranially injected with 2 × 10(5) hMAPCs or hMSCs 2 days after stroke and followed for up to 28 days. We could not detect long-term engraftment of either cell population. However, in comparison with PBS-treated animals, hMSC and hMAPC grafted animals demonstrated significantly decreased loss of brain tissue. This was associated with increased angiogenesis, diminished inflammation and a glial-scar inhibitory effect. Moreover, enhanced proliferation of cells in the subventricular zone (SVZ) and survival of newly generated neuroblasts was observed. Interestingly, these neuroprotective effects were more pronounced in the group of animals treated with hMAPCs in comparison with hMSCs. Our results establish cell therapy with hMAPCs and hMSCs as a promising strategy for the treatment of stroke.
format	article
author	Silvia Mora-Lee Ma Salomé Sirerol-Piquer María Gutiérrez-Pérez Ulises Gomez-Pinedo Valerie D Roobrouck Tania López Mayte Casado-Nieto Gloria Abizanda Maria Teresa Rabena Catherine Verfaille Felipe Prósper Jose Manuel García-Verdugo
author_facet	Silvia Mora-Lee Ma Salomé Sirerol-Piquer María Gutiérrez-Pérez Ulises Gomez-Pinedo Valerie D Roobrouck Tania López Mayte Casado-Nieto Gloria Abizanda Maria Teresa Rabena Catherine Verfaille Felipe Prósper Jose Manuel García-Verdugo
author_sort	Silvia Mora-Lee
title	Therapeutic effects of hMAPC and hMSC transplantation after stroke in mice.
title_short	Therapeutic effects of hMAPC and hMSC transplantation after stroke in mice.
title_full	Therapeutic effects of hMAPC and hMSC transplantation after stroke in mice.
title_fullStr	Therapeutic effects of hMAPC and hMSC transplantation after stroke in mice.
title_full_unstemmed	Therapeutic effects of hMAPC and hMSC transplantation after stroke in mice.
title_sort	therapeutic effects of hmapc and hmsc transplantation after stroke in mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2ea8361a7bc74b268f115131262f70dc
work_keys_str_mv	AT silviamoralee therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice AT masalomesirerolpiquer therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice AT mariagutierrezperez therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice AT ulisesgomezpinedo therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice AT valeriedroobrouck therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice AT tanialopez therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice AT maytecasadonieto therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice AT gloriaabizanda therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice AT mariateresarabena therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice AT catherineverfaille therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice AT felipeprosper therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice AT josemanuelgarciaverdugo therapeuticeffectsofhmapcandhmsctransplantationafterstrokeinmice
_version_	1718423906715435008

Therapeutic effects of hMAPC and hMSC transplantation after stroke in mice.

Ejemplares similares