Urinary microRNA biomarkers for detecting the presence of esophageal cancer

Abstract Esophageal cancer (EC) including esophageal squamous cell carcinoma (ESCC) and adenocarcinoma (EAC) generally exhibits poor prognosis; hence, a noninvasive biomarker enabling early detection is necessary. Age- and sex-matched 150 healthy controls (HCs) and 43 patients with ESCC were randoml...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yusuke Okuda, Takaya Shimura, Hiroyasu Iwasaki, Shigeki Fukusada, Ruriko Nishigaki, Mika Kitagawa, Takahito Katano, Yasuyuki Okamoto, Tamaki Yamada, Shin-ichi Horike, Hiromi Kataoka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2eb029746c974d9c8599c5dd598951e9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2eb029746c974d9c8599c5dd598951e9
record_format	dspace
spelling	oai:doaj.org-article:2eb029746c974d9c8599c5dd598951e92021-12-02T16:45:47ZUrinary microRNA biomarkers for detecting the presence of esophageal cancer10.1038/s41598-021-87925-12045-2322https://doaj.org/article/2eb029746c974d9c8599c5dd598951e92021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87925-1https://doaj.org/toc/2045-2322Abstract Esophageal cancer (EC) including esophageal squamous cell carcinoma (ESCC) and adenocarcinoma (EAC) generally exhibits poor prognosis; hence, a noninvasive biomarker enabling early detection is necessary. Age- and sex-matched 150 healthy controls (HCs) and 43 patients with ESCC were randomly divided into two groups: 9 individuals in the discovery cohort for microarray analysis and 184 individuals in the training/test cohort with cross-validation for qRT-PCR analysis. Using 152 urine samples (144 HCs and 8 EACs), we validated the urinary miRNA biomarkers for EAC diagnosis. Among eight miRNAs selected in the discovery cohort, urinary levels of five miRNAs (miR-1273f, miR-619-5p, miR-150-3p, miR-4327, and miR-3135b) were significantly higher in the ESCC group than in the HC group, in the training/test cohort. Consistently, these five urinary miRNAs were significantly different between HC and ESCC in both training and test sets. Especially, urinary miR-1273f and miR-619-5p showed excellent values of area under the curve (AUC) ≥ 0.80 for diagnosing stage I ESCC. Similarly, the EAC group had significantly higher urinary levels of these five miRNAs than the HC group, with AUC values of approximately 0.80. The present study established novel urinary miRNA biomarkers that can early detect ESCC and EAC.Yusuke OkudaTakaya ShimuraHiroyasu IwasakiShigeki FukusadaRuriko NishigakiMika KitagawaTakahito KatanoYasuyuki OkamotoTamaki YamadaShin-ichi HorikeHiromi KataokaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yusuke Okuda Takaya Shimura Hiroyasu Iwasaki Shigeki Fukusada Ruriko Nishigaki Mika Kitagawa Takahito Katano Yasuyuki Okamoto Tamaki Yamada Shin-ichi Horike Hiromi Kataoka Urinary microRNA biomarkers for detecting the presence of esophageal cancer
description	Abstract Esophageal cancer (EC) including esophageal squamous cell carcinoma (ESCC) and adenocarcinoma (EAC) generally exhibits poor prognosis; hence, a noninvasive biomarker enabling early detection is necessary. Age- and sex-matched 150 healthy controls (HCs) and 43 patients with ESCC were randomly divided into two groups: 9 individuals in the discovery cohort for microarray analysis and 184 individuals in the training/test cohort with cross-validation for qRT-PCR analysis. Using 152 urine samples (144 HCs and 8 EACs), we validated the urinary miRNA biomarkers for EAC diagnosis. Among eight miRNAs selected in the discovery cohort, urinary levels of five miRNAs (miR-1273f, miR-619-5p, miR-150-3p, miR-4327, and miR-3135b) were significantly higher in the ESCC group than in the HC group, in the training/test cohort. Consistently, these five urinary miRNAs were significantly different between HC and ESCC in both training and test sets. Especially, urinary miR-1273f and miR-619-5p showed excellent values of area under the curve (AUC) ≥ 0.80 for diagnosing stage I ESCC. Similarly, the EAC group had significantly higher urinary levels of these five miRNAs than the HC group, with AUC values of approximately 0.80. The present study established novel urinary miRNA biomarkers that can early detect ESCC and EAC.
format	article
author	Yusuke Okuda Takaya Shimura Hiroyasu Iwasaki Shigeki Fukusada Ruriko Nishigaki Mika Kitagawa Takahito Katano Yasuyuki Okamoto Tamaki Yamada Shin-ichi Horike Hiromi Kataoka
author_facet	Yusuke Okuda Takaya Shimura Hiroyasu Iwasaki Shigeki Fukusada Ruriko Nishigaki Mika Kitagawa Takahito Katano Yasuyuki Okamoto Tamaki Yamada Shin-ichi Horike Hiromi Kataoka
author_sort	Yusuke Okuda
title	Urinary microRNA biomarkers for detecting the presence of esophageal cancer
title_short	Urinary microRNA biomarkers for detecting the presence of esophageal cancer
title_full	Urinary microRNA biomarkers for detecting the presence of esophageal cancer
title_fullStr	Urinary microRNA biomarkers for detecting the presence of esophageal cancer
title_full_unstemmed	Urinary microRNA biomarkers for detecting the presence of esophageal cancer
title_sort	urinary microrna biomarkers for detecting the presence of esophageal cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2eb029746c974d9c8599c5dd598951e9
work_keys_str_mv	AT yusukeokuda urinarymicrornabiomarkersfordetectingthepresenceofesophagealcancer AT takayashimura urinarymicrornabiomarkersfordetectingthepresenceofesophagealcancer AT hiroyasuiwasaki urinarymicrornabiomarkersfordetectingthepresenceofesophagealcancer AT shigekifukusada urinarymicrornabiomarkersfordetectingthepresenceofesophagealcancer AT rurikonishigaki urinarymicrornabiomarkersfordetectingthepresenceofesophagealcancer AT mikakitagawa urinarymicrornabiomarkersfordetectingthepresenceofesophagealcancer AT takahitokatano urinarymicrornabiomarkersfordetectingthepresenceofesophagealcancer AT yasuyukiokamoto urinarymicrornabiomarkersfordetectingthepresenceofesophagealcancer AT tamakiyamada urinarymicrornabiomarkersfordetectingthepresenceofesophagealcancer AT shinichihorike urinarymicrornabiomarkersfordetectingthepresenceofesophagealcancer AT hiromikataoka urinarymicrornabiomarkersfordetectingthepresenceofesophagealcancer
_version_	1718383445830270976

Urinary microRNA biomarkers for detecting the presence of esophageal cancer

Ejemplares similares