Model for the Controlled Synthesis of O-Antigen Repeat Units Involving the WaaL Ligase

ABSTRACT The Wzx/Wzy O-antigen pathway involves synthesis of a repeat unit (O unit) consisting of 3 to 8 sugars on an inner-membrane-embedded lipid carrier. These O units are translocated across the membrane to its periplasmic face by Wzx, while retaining linkage to the carrier, and then polymerized...

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Autores principales:	Yaoqin Hong, Peter R. Reeves
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2eb7142a319c4ec0834f4fff513cbe82
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id	oai:doaj.org-article:2eb7142a319c4ec0834f4fff513cbe82
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spelling	oai:doaj.org-article:2eb7142a319c4ec0834f4fff513cbe822021-11-15T15:21:39ZModel for the Controlled Synthesis of O-Antigen Repeat Units Involving the WaaL Ligase10.1128/mSphere.00074-152379-5042https://doaj.org/article/2eb7142a319c4ec0834f4fff513cbe822016-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00074-15https://doaj.org/toc/2379-5042ABSTRACT The Wzx/Wzy O-antigen pathway involves synthesis of a repeat unit (O unit) consisting of 3 to 8 sugars on an inner-membrane-embedded lipid carrier. These O units are translocated across the membrane to its periplasmic face by Wzx, while retaining linkage to the carrier, and then polymerized by Wzy to O-antigen polymer, which WaaL ligase transfers to a lipopolysaccharide precursor to complete lipopolysaccharide synthesis, concomitantly releasing the lipid carrier. This lipid carrier is also used for peptidoglycan assembly, and sequestration is known to be toxic. Thus, O-unit synthesis must involve precise regulation to meet demand but avoid overproduction. Here we show that loss of WaaL reverses a known growth defect in a Salmonella mutant that otherwise accumulates O-unit intermediates and propose that WaaL is also involved in a novel feedback mechanism to regulate O-unit synthesis, based on the availability of O units on the periplasmic face of the membrane.Yaoqin HongPeter R. ReevesAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSphere, Vol 1, Iss 1 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Yaoqin Hong Peter R. Reeves Model for the Controlled Synthesis of O-Antigen Repeat Units Involving the WaaL Ligase
description	ABSTRACT The Wzx/Wzy O-antigen pathway involves synthesis of a repeat unit (O unit) consisting of 3 to 8 sugars on an inner-membrane-embedded lipid carrier. These O units are translocated across the membrane to its periplasmic face by Wzx, while retaining linkage to the carrier, and then polymerized by Wzy to O-antigen polymer, which WaaL ligase transfers to a lipopolysaccharide precursor to complete lipopolysaccharide synthesis, concomitantly releasing the lipid carrier. This lipid carrier is also used for peptidoglycan assembly, and sequestration is known to be toxic. Thus, O-unit synthesis must involve precise regulation to meet demand but avoid overproduction. Here we show that loss of WaaL reverses a known growth defect in a Salmonella mutant that otherwise accumulates O-unit intermediates and propose that WaaL is also involved in a novel feedback mechanism to regulate O-unit synthesis, based on the availability of O units on the periplasmic face of the membrane.
format	article
author	Yaoqin Hong Peter R. Reeves
author_facet	Yaoqin Hong Peter R. Reeves
author_sort	Yaoqin Hong
title	Model for the Controlled Synthesis of O-Antigen Repeat Units Involving the WaaL Ligase
title_short	Model for the Controlled Synthesis of O-Antigen Repeat Units Involving the WaaL Ligase
title_full	Model for the Controlled Synthesis of O-Antigen Repeat Units Involving the WaaL Ligase
title_fullStr	Model for the Controlled Synthesis of O-Antigen Repeat Units Involving the WaaL Ligase
title_full_unstemmed	Model for the Controlled Synthesis of O-Antigen Repeat Units Involving the WaaL Ligase
title_sort	model for the controlled synthesis of o-antigen repeat units involving the waal ligase
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/2eb7142a319c4ec0834f4fff513cbe82
work_keys_str_mv	AT yaoqinhong modelforthecontrolledsynthesisofoantigenrepeatunitsinvolvingthewaalligase AT peterrreeves modelforthecontrolledsynthesisofoantigenrepeatunitsinvolvingthewaalligase
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Model for the Controlled Synthesis of O-Antigen Repeat Units Involving the WaaL Ligase

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