Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018

Abstract New circulating Enterovirus (EV) strains often emerge through recombination. Upsurges of recombinant non-polio enteroviruses (NPEVs) associated with neurologic manifestations such as EVA71 or Echovirus 30 (E30) are a growing public health concern in Europe. Only a few complete genomes of EV...

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Autores principales:	Fabiana Gámbaro, Ana Belén Pérez, Eduardo Agüera, Matthieu Prot, Luis Martínez-Martínez, María Cabrerizo, Etienne Simon-Loriere, Maria Dolores Fernandez-Garcia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ec4c97ec52149b19ba741dd18e2b38e
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spelling	oai:doaj.org-article:2ec4c97ec52149b19ba741dd18e2b38e2021-11-08T10:46:13ZGenomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–201810.1038/s41598-021-01053-42045-2322https://doaj.org/article/2ec4c97ec52149b19ba741dd18e2b38e2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01053-4https://doaj.org/toc/2045-2322Abstract New circulating Enterovirus (EV) strains often emerge through recombination. Upsurges of recombinant non-polio enteroviruses (NPEVs) associated with neurologic manifestations such as EVA71 or Echovirus 30 (E30) are a growing public health concern in Europe. Only a few complete genomes of EVs circulating in Spain are available in public databases, making it difficult to address the emergence of recombinant EVs, understand their evolutionary relatedness and the possible implication in human disease. We have used metagenomic (untargeted) NGS to generate full-length EV genomes from CSF samples of EV-positive aseptic meningitis cases in Southern Spain between 2015 and 2018. Our analyses reveal the co-circulation of multiple Enterovirus B (EV-B) types (E6, E11, E13 and E30), including a novel E13 recombinant form. We observed a genetic turnover where emergent lineages (C1 for E6 and I [tentatively proposed in this study] for E30) replaced previous lineages circulating in Spain, some concomitant with outbreaks in other parts of Europe. Metagenomic sequencing provides an effective approach for the analysis of EV genomes directly from PCR-positive CSF samples. The detection of a novel, disease-associated, recombinant form emphasizes the importance of genomic surveillance to monitor spread and evolution of EVs.Fabiana GámbaroAna Belén PérezEduardo AgüeraMatthieu ProtLuis Martínez-MartínezMaría CabrerizoEtienne Simon-LoriereMaria Dolores Fernandez-GarciaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fabiana Gámbaro Ana Belén Pérez Eduardo Agüera Matthieu Prot Luis Martínez-Martínez María Cabrerizo Etienne Simon-Loriere Maria Dolores Fernandez-Garcia Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018
description	Abstract New circulating Enterovirus (EV) strains often emerge through recombination. Upsurges of recombinant non-polio enteroviruses (NPEVs) associated with neurologic manifestations such as EVA71 or Echovirus 30 (E30) are a growing public health concern in Europe. Only a few complete genomes of EVs circulating in Spain are available in public databases, making it difficult to address the emergence of recombinant EVs, understand their evolutionary relatedness and the possible implication in human disease. We have used metagenomic (untargeted) NGS to generate full-length EV genomes from CSF samples of EV-positive aseptic meningitis cases in Southern Spain between 2015 and 2018. Our analyses reveal the co-circulation of multiple Enterovirus B (EV-B) types (E6, E11, E13 and E30), including a novel E13 recombinant form. We observed a genetic turnover where emergent lineages (C1 for E6 and I [tentatively proposed in this study] for E30) replaced previous lineages circulating in Spain, some concomitant with outbreaks in other parts of Europe. Metagenomic sequencing provides an effective approach for the analysis of EV genomes directly from PCR-positive CSF samples. The detection of a novel, disease-associated, recombinant form emphasizes the importance of genomic surveillance to monitor spread and evolution of EVs.
format	article
author	Fabiana Gámbaro Ana Belén Pérez Eduardo Agüera Matthieu Prot Luis Martínez-Martínez María Cabrerizo Etienne Simon-Loriere Maria Dolores Fernandez-Garcia
author_facet	Fabiana Gámbaro Ana Belén Pérez Eduardo Agüera Matthieu Prot Luis Martínez-Martínez María Cabrerizo Etienne Simon-Loriere Maria Dolores Fernandez-Garcia
author_sort	Fabiana Gámbaro
title	Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018
title_short	Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018
title_full	Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018
title_fullStr	Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018
title_full_unstemmed	Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018
title_sort	genomic surveillance of enterovirus associated with aseptic meningitis cases in southern spain, 2015–2018
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2ec4c97ec52149b19ba741dd18e2b38e
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Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018

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