Radiofrequency remote control of thermolysin activity

Abstract The majority of biological processes are regulated by enzymes, precise control over specific enzymes could create the potential for controlling cellular processes remotely. We show that the thermophilic enzyme thermolysin can be remotely activated in 17.76 MHz radiofrequency (RF) fields whe...

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Autores principales:	Christian B. Collins, Ryan A. Riskowski, Christopher J. Ackerson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ec73bc33b3443718a0c47ffe94e8fb6
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spelling	oai:doaj.org-article:2ec73bc33b3443718a0c47ffe94e8fb62021-12-02T17:05:45ZRadiofrequency remote control of thermolysin activity10.1038/s41598-021-85611-w2045-2322https://doaj.org/article/2ec73bc33b3443718a0c47ffe94e8fb62021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85611-whttps://doaj.org/toc/2045-2322Abstract The majority of biological processes are regulated by enzymes, precise control over specific enzymes could create the potential for controlling cellular processes remotely. We show that the thermophilic enzyme thermolysin can be remotely activated in 17.76 MHz radiofrequency (RF) fields when covalently attached to 6.1 nm gold coated magnetite nanoparticles. Without raising the bulk solution temperature, we observe enzyme activity as if the solution was 16 ± 2 °C warmer in RF fields—an increase in enzymatic rate of 129 ± 8%. Kinetics studies show that the activity increase of the enzyme is consistent with the induced fit of a hot enzyme with cold substrate.Christian B. CollinsRyan A. RiskowskiChristopher J. AckersonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian B. Collins Ryan A. Riskowski Christopher J. Ackerson Radiofrequency remote control of thermolysin activity
description	Abstract The majority of biological processes are regulated by enzymes, precise control over specific enzymes could create the potential for controlling cellular processes remotely. We show that the thermophilic enzyme thermolysin can be remotely activated in 17.76 MHz radiofrequency (RF) fields when covalently attached to 6.1 nm gold coated magnetite nanoparticles. Without raising the bulk solution temperature, we observe enzyme activity as if the solution was 16 ± 2 °C warmer in RF fields—an increase in enzymatic rate of 129 ± 8%. Kinetics studies show that the activity increase of the enzyme is consistent with the induced fit of a hot enzyme with cold substrate.
format	article
author	Christian B. Collins Ryan A. Riskowski Christopher J. Ackerson
author_facet	Christian B. Collins Ryan A. Riskowski Christopher J. Ackerson
author_sort	Christian B. Collins
title	Radiofrequency remote control of thermolysin activity
title_short	Radiofrequency remote control of thermolysin activity
title_full	Radiofrequency remote control of thermolysin activity
title_fullStr	Radiofrequency remote control of thermolysin activity
title_full_unstemmed	Radiofrequency remote control of thermolysin activity
title_sort	radiofrequency remote control of thermolysin activity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2ec73bc33b3443718a0c47ffe94e8fb6
work_keys_str_mv	AT christianbcollins radiofrequencyremotecontrolofthermolysinactivity AT ryanariskowski radiofrequencyremotecontrolofthermolysinactivity AT christopherjackerson radiofrequencyremotecontrolofthermolysinactivity
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Radiofrequency remote control of thermolysin activity

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