Hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic Iron oxide nanoparticles

Abstract Background Superparamagnetic iron oxide (SPIO) nanoparticles have been widely used in several biomedical engineering in vivo. Although various surface modifications have been made to these non-biodegradable nanoparticles to make them more biocompatible, their toxic potential still remains a...

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Autores principales:	Yong Hwa Hwang, Youn-Jung Kim, Dong Yun Lee
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Negative contrast agents Superparamagnetic iron oxide (SPIO) Heparin Dextran Cytotoxicity Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/2ed3956aa1164e8ea923bb9cc4ceae11
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spelling	oai:doaj.org-article:2ed3956aa1164e8ea923bb9cc4ceae112021-11-07T12:07:19ZHepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic Iron oxide nanoparticles10.1186/s40824-021-00241-72055-7124https://doaj.org/article/2ed3956aa1164e8ea923bb9cc4ceae112021-11-01T00:00:00Zhttps://doi.org/10.1186/s40824-021-00241-7https://doaj.org/toc/2055-7124Abstract Background Superparamagnetic iron oxide (SPIO) nanoparticles have been widely used in several biomedical engineering in vivo. Although various surface modifications have been made to these non-biodegradable nanoparticles to make them more biocompatible, their toxic potential still remains a major concern. Method In this study, we newly developed unfractionated heparin (UFH)-coated and low molecular weight heparin (LMWH)-coated SPIO nanoparticles through surface modification engineering, which was compared with commercially available dextran-coated SPIO nanoparticles. Their toxicity such as cytotoxicity, single cell gel electrophoresis (SCGE) comet assay, intracellular reactive oxygen species (ROS) content and cellular apoptosis was evaluated to hepatic HepG2 and renal HK-2 cells. Results When UFH-, LMWH- or dextran-coated SPIO nanoparticles were applied, they did not affect the viability of HepG2 cell. However, HK-2 cells were more sensitive to dextran-coated SPIO nanoparticles than others. In genotoxicity assay using SCGE comet, DNA tail moment values in the groups treated with dextran- and LMWH-coated SPIO nanoparticles significantly increased. However, UFH-coated SPIO nanoparticles was only significantly lowing DNA tail moment value. In addition, UFH-coated SPIO nanoparticles had lower cytotoxicity in HepG2 and HK-2 cells compared to dextran-coated SPIO nanoparticles, especially in terms of apoptosis and intracellular ROS production. Conclusions Collectively, it is possible that UFH- coated SPIO nanoparticles can be used as alternative negative contrast agents.Yong Hwa HwangYoun-Jung KimDong Yun LeeBMCarticleNegative contrast agentsSuperparamagnetic iron oxide (SPIO)HeparinDextranCytotoxicityMedical technologyR855-855.5ENBiomaterials Research, Vol 25, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Negative contrast agents Superparamagnetic iron oxide (SPIO) Heparin Dextran Cytotoxicity Medical technology R855-855.5
spellingShingle	Negative contrast agents Superparamagnetic iron oxide (SPIO) Heparin Dextran Cytotoxicity Medical technology R855-855.5 Yong Hwa Hwang Youn-Jung Kim Dong Yun Lee Hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic Iron oxide nanoparticles
description	Abstract Background Superparamagnetic iron oxide (SPIO) nanoparticles have been widely used in several biomedical engineering in vivo. Although various surface modifications have been made to these non-biodegradable nanoparticles to make them more biocompatible, their toxic potential still remains a major concern. Method In this study, we newly developed unfractionated heparin (UFH)-coated and low molecular weight heparin (LMWH)-coated SPIO nanoparticles through surface modification engineering, which was compared with commercially available dextran-coated SPIO nanoparticles. Their toxicity such as cytotoxicity, single cell gel electrophoresis (SCGE) comet assay, intracellular reactive oxygen species (ROS) content and cellular apoptosis was evaluated to hepatic HepG2 and renal HK-2 cells. Results When UFH-, LMWH- or dextran-coated SPIO nanoparticles were applied, they did not affect the viability of HepG2 cell. However, HK-2 cells were more sensitive to dextran-coated SPIO nanoparticles than others. In genotoxicity assay using SCGE comet, DNA tail moment values in the groups treated with dextran- and LMWH-coated SPIO nanoparticles significantly increased. However, UFH-coated SPIO nanoparticles was only significantly lowing DNA tail moment value. In addition, UFH-coated SPIO nanoparticles had lower cytotoxicity in HepG2 and HK-2 cells compared to dextran-coated SPIO nanoparticles, especially in terms of apoptosis and intracellular ROS production. Conclusions Collectively, it is possible that UFH- coated SPIO nanoparticles can be used as alternative negative contrast agents.
format	article
author	Yong Hwa Hwang Youn-Jung Kim Dong Yun Lee
author_facet	Yong Hwa Hwang Youn-Jung Kim Dong Yun Lee
author_sort	Yong Hwa Hwang
title	Hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic Iron oxide nanoparticles
title_short	Hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic Iron oxide nanoparticles
title_full	Hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic Iron oxide nanoparticles
title_fullStr	Hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic Iron oxide nanoparticles
title_full_unstemmed	Hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic Iron oxide nanoparticles
title_sort	hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic iron oxide nanoparticles
publisher	BMC
publishDate	2021
url	https://doaj.org/article/2ed3956aa1164e8ea923bb9cc4ceae11
work_keys_str_mv	AT yonghwahwang hepaticandrenalcellularcytotoxiceffectsofheparincoatedsuperparamagneticironoxidenanoparticles AT younjungkim hepaticandrenalcellularcytotoxiceffectsofheparincoatedsuperparamagneticironoxidenanoparticles AT dongyunlee hepaticandrenalcellularcytotoxiceffectsofheparincoatedsuperparamagneticironoxidenanoparticles
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Hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic Iron oxide nanoparticles

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