Optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids

Abstract Knowledge of optical properties, such as the refractive index (RI), of biological tissues is important in optical imaging, as they influence the distribution and propagation of light in tissue. To accurately study the response of cancerous cells to drugs, optimised imaging protocols are req...

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Auteurs principaux:	Neelam Hari, Priyanka Patel, Jacqueline Ross, Kevin Hicks, Frédérique Vanholsbeeck
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2019
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/2eeb09613b95477c85f3f88d35fbe5ee
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spelling	oai:doaj.org-article:2eeb09613b95477c85f3f88d35fbe5ee2021-12-02T16:07:53ZOptical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids10.1038/s41598-019-47000-22045-2322https://doaj.org/article/2eeb09613b95477c85f3f88d35fbe5ee2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47000-2https://doaj.org/toc/2045-2322Abstract Knowledge of optical properties, such as the refractive index (RI), of biological tissues is important in optical imaging, as they influence the distribution and propagation of light in tissue. To accurately study the response of cancerous cells to drugs, optimised imaging protocols are required. This study uses a simple custom-built spectral domain optical coherence tomography (OCT) system to conduct RI measurements of multicellular spheroids, three-dimensional (3D) in-vitro culture systems, of the cell line HCT116. The spheroid RIs are compared to study the effect of growth over time. To improve confocal microscopy imaging protocols, two immersion media (glycerol and ScaleView-A2) matching the spheroid RIs were trialled, with the aim to reduce the RI mismatch between the spheroid and the immersion medium and thus improve imaging depth with confocal microscopy. ScaleView-A2 (n = 1.380) aided in achieving greater depths of imaging of the multicellular spheroids under confocal microscopy. This improvement in imaging depth confirmed the utility of our RI measurements, proving the promising outlook of OCT as a complementary tool to microscopy in cancer research.Neelam HariPriyanka PatelJacqueline RossKevin HicksFrédérique VanholsbeeckNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Neelam Hari Priyanka Patel Jacqueline Ross Kevin Hicks Frédérique Vanholsbeeck Optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids
description	Abstract Knowledge of optical properties, such as the refractive index (RI), of biological tissues is important in optical imaging, as they influence the distribution and propagation of light in tissue. To accurately study the response of cancerous cells to drugs, optimised imaging protocols are required. This study uses a simple custom-built spectral domain optical coherence tomography (OCT) system to conduct RI measurements of multicellular spheroids, three-dimensional (3D) in-vitro culture systems, of the cell line HCT116. The spheroid RIs are compared to study the effect of growth over time. To improve confocal microscopy imaging protocols, two immersion media (glycerol and ScaleView-A2) matching the spheroid RIs were trialled, with the aim to reduce the RI mismatch between the spheroid and the immersion medium and thus improve imaging depth with confocal microscopy. ScaleView-A2 (n = 1.380) aided in achieving greater depths of imaging of the multicellular spheroids under confocal microscopy. This improvement in imaging depth confirmed the utility of our RI measurements, proving the promising outlook of OCT as a complementary tool to microscopy in cancer research.
format	article
author	Neelam Hari Priyanka Patel Jacqueline Ross Kevin Hicks Frédérique Vanholsbeeck
author_facet	Neelam Hari Priyanka Patel Jacqueline Ross Kevin Hicks Frédérique Vanholsbeeck
author_sort	Neelam Hari
title	Optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids
title_short	Optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids
title_full	Optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids
title_fullStr	Optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids
title_full_unstemmed	Optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids
title_sort	optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/2eeb09613b95477c85f3f88d35fbe5ee
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Optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids

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