Treatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study

Rodrigo Mardones,1 Daniel Camacho,1,2 Francisco Monsalvo1 Nicolás Zulch,1 Claudio Jofre,3 José J Minguell3 1Department of Orthopedics, Clínica Las Condes, Santiago, Chile; 2Department of Orthopedics, Instituto Traumatológico, Santiago, Chile; 3Centro de Te...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mardones R, Camacho D, Monsalvo F, Zulch N, Jofre C, Minguell JJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Avascular Necrosis Treatment Ex vivo expanded MSC A proof-of-concept study. Cytology QH573-671
Acceso en línea:	https://doaj.org/article/2ef8c4798a7d4487948a21c9b3646714
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2ef8c4798a7d4487948a21c9b3646714
record_format	dspace
spelling	oai:doaj.org-article:2ef8c4798a7d4487948a21c9b36467142021-12-02T05:47:41ZTreatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study1178-6957https://doaj.org/article/2ef8c4798a7d4487948a21c9b36467142019-03-01T00:00:00Zhttps://www.dovepress.com/treatment-of-osteonecrosis-of-the-femoral-head-by-core-decompression-a-peer-reviewed-article-SCCAAhttps://doaj.org/toc/1178-6957Rodrigo Mardones,1 Daniel Camacho,1,2 Francisco Monsalvo1 Nicolás Zulch,1 Claudio Jofre,3 José J Minguell3 1Department of Orthopedics, Clínica Las Condes, Santiago, Chile; 2Department of Orthopedics, Instituto Traumatológico, Santiago, Chile; 3Centro de Terapia Regenerativa Celular, Clínica Las Condes, Santiago, Chile Background: Based on several attributes involved in bone formation, bone marrow-resident mesenchymal stem cells (MSCs) have been employed in the treatment of patients suffering from femoral head osteonecrosis. Due to the low content of MSCs in the bone marrow, ex vivo expansion procedures are utilized to increase the cell number. Customarily, before administration of the resulting expanded cell product MSCs to the patient, its cellular identity is usually evaluated according to a set of “minimal phenotypic” markers, which are not modified by ex vivo processing. However, MSC functional (“reparative”) markers, which are severely impaired along the ex vivo expansion routine, are usually not assessed.Patients and methods: In this proof-of-concept study, a cohort of five avascular osteonecrosis patients received an instillation of ex vivo-expanded autologous MSCs, manufactured under controlled conditions, with an aim to protect their functional (“reparative”) capacity.Results and conclusion: Outcomes of this study confirmed the safety and effectiveness of the MSC-based therapy used. After a follow-up period (19–54 months), in all patients, the hip function was significantly improved and pain intensity markedly reduced. As a corollary, no patient required hip arthroplasty. Keywords: avascular necrosis, femoral head, osteonecrosis, mesenchymal stem cell-based therapyMardones RCamacho DMonsalvo FZulch NJofre CMinguell JJDove Medical PressarticleAvascular NecrosisTreatmentEx vivo expanded MSCA proof-of-concept study.CytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol Volume 12, Pp 11-16 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Avascular Necrosis Treatment Ex vivo expanded MSC A proof-of-concept study. Cytology QH573-671
spellingShingle	Avascular Necrosis Treatment Ex vivo expanded MSC A proof-of-concept study. Cytology QH573-671 Mardones R Camacho D Monsalvo F Zulch N Jofre C Minguell JJ Treatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study
description	Rodrigo Mardones,1 Daniel Camacho,1,2 Francisco Monsalvo1 Nicolás Zulch,1 Claudio Jofre,3 José J Minguell3 1Department of Orthopedics, Clínica Las Condes, Santiago, Chile; 2Department of Orthopedics, Instituto Traumatológico, Santiago, Chile; 3Centro de Terapia Regenerativa Celular, Clínica Las Condes, Santiago, Chile Background: Based on several attributes involved in bone formation, bone marrow-resident mesenchymal stem cells (MSCs) have been employed in the treatment of patients suffering from femoral head osteonecrosis. Due to the low content of MSCs in the bone marrow, ex vivo expansion procedures are utilized to increase the cell number. Customarily, before administration of the resulting expanded cell product MSCs to the patient, its cellular identity is usually evaluated according to a set of “minimal phenotypic” markers, which are not modified by ex vivo processing. However, MSC functional (“reparative”) markers, which are severely impaired along the ex vivo expansion routine, are usually not assessed.Patients and methods: In this proof-of-concept study, a cohort of five avascular osteonecrosis patients received an instillation of ex vivo-expanded autologous MSCs, manufactured under controlled conditions, with an aim to protect their functional (“reparative”) capacity.Results and conclusion: Outcomes of this study confirmed the safety and effectiveness of the MSC-based therapy used. After a follow-up period (19–54 months), in all patients, the hip function was significantly improved and pain intensity markedly reduced. As a corollary, no patient required hip arthroplasty. Keywords: avascular necrosis, femoral head, osteonecrosis, mesenchymal stem cell-based therapy
format	article
author	Mardones R Camacho D Monsalvo F Zulch N Jofre C Minguell JJ
author_facet	Mardones R Camacho D Monsalvo F Zulch N Jofre C Minguell JJ
author_sort	Mardones R
title	Treatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study
title_short	Treatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study
title_full	Treatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study
title_fullStr	Treatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study
title_full_unstemmed	Treatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study
title_sort	treatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/2ef8c4798a7d4487948a21c9b3646714
work_keys_str_mv	AT mardonesr treatmentofosteonecrosisofthefemoralheadbycoredecompressionandimplantationoffullyfunctionalexvivoexpandedbonemarrowderivedmesenchymalstemcellsaproofofconceptstudy AT camachod treatmentofosteonecrosisofthefemoralheadbycoredecompressionandimplantationoffullyfunctionalexvivoexpandedbonemarrowderivedmesenchymalstemcellsaproofofconceptstudy AT monsalvof treatmentofosteonecrosisofthefemoralheadbycoredecompressionandimplantationoffullyfunctionalexvivoexpandedbonemarrowderivedmesenchymalstemcellsaproofofconceptstudy AT zulchn treatmentofosteonecrosisofthefemoralheadbycoredecompressionandimplantationoffullyfunctionalexvivoexpandedbonemarrowderivedmesenchymalstemcellsaproofofconceptstudy AT jofrec treatmentofosteonecrosisofthefemoralheadbycoredecompressionandimplantationoffullyfunctionalexvivoexpandedbonemarrowderivedmesenchymalstemcellsaproofofconceptstudy AT minguelljj treatmentofosteonecrosisofthefemoralheadbycoredecompressionandimplantationoffullyfunctionalexvivoexpandedbonemarrowderivedmesenchymalstemcellsaproofofconceptstudy
_version_	1718400220955410432

Treatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study

Ejemplares similares