A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation

Abstract The development of livers-on-a-chip aims to provide pharmaceutical companies with reliable systems to perform drug screening and toxicological studies. To that end, microfluidic systems are engineered to mimic the functions and architecture of this organ. In this context we have designed a...

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Autores principales:	Manon Boul, Nassima Benzoubir, Antonietta Messina, Rasta Ghasemi, Ismail Ben Mosbah, Jean-Charles Duclos-Vallée, Anne Dubart-Kupperschmitt, Bruno Le Pioufle
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2efdb3a409f7419390cda61f1cba0a51
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spelling	oai:doaj.org-article:2efdb3a409f7419390cda61f1cba0a512021-12-02T18:34:07ZA versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation10.1038/s41598-021-92011-72045-2322https://doaj.org/article/2efdb3a409f7419390cda61f1cba0a512021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92011-7https://doaj.org/toc/2045-2322Abstract The development of livers-on-a-chip aims to provide pharmaceutical companies with reliable systems to perform drug screening and toxicological studies. To that end, microfluidic systems are engineered to mimic the functions and architecture of this organ. In this context we have designed a device that reproduces series of liver microarchitectures, each permitting the 3D culture of hepatocytes by confining them to a chamber that is separated from the medium conveying channel by very thin slits. We modified the structure to ensure its compatibility with the culture of hepatocytes from different sources. Our device was adapted to the migratory and adhesion properties of the human HepaRG cell line at various stages of differentiation. Using this device, it was possible to keep the cells alive for more than 14 days, during which they achieved a 3D organisation and acquired or maintained their differentiation into hepatocytes. Albumin secretion as well as functional bile canaliculi were confirmed on the liver-on-a-chip. Finally, an acetaminophen toxicological assay was performed. With its multiple micro-chambers for hepatocyte culture, this microfluidic device architecture offers a promising opportunity to provide new tools for drug screening applications.Manon BoulNassima BenzoubirAntonietta MessinaRasta GhasemiIsmail Ben MosbahJean-Charles Duclos-ValléeAnne Dubart-KupperschmittBruno Le PioufleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manon Boul Nassima Benzoubir Antonietta Messina Rasta Ghasemi Ismail Ben Mosbah Jean-Charles Duclos-Vallée Anne Dubart-Kupperschmitt Bruno Le Pioufle A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation
description	Abstract The development of livers-on-a-chip aims to provide pharmaceutical companies with reliable systems to perform drug screening and toxicological studies. To that end, microfluidic systems are engineered to mimic the functions and architecture of this organ. In this context we have designed a device that reproduces series of liver microarchitectures, each permitting the 3D culture of hepatocytes by confining them to a chamber that is separated from the medium conveying channel by very thin slits. We modified the structure to ensure its compatibility with the culture of hepatocytes from different sources. Our device was adapted to the migratory and adhesion properties of the human HepaRG cell line at various stages of differentiation. Using this device, it was possible to keep the cells alive for more than 14 days, during which they achieved a 3D organisation and acquired or maintained their differentiation into hepatocytes. Albumin secretion as well as functional bile canaliculi were confirmed on the liver-on-a-chip. Finally, an acetaminophen toxicological assay was performed. With its multiple micro-chambers for hepatocyte culture, this microfluidic device architecture offers a promising opportunity to provide new tools for drug screening applications.
format	article
author	Manon Boul Nassima Benzoubir Antonietta Messina Rasta Ghasemi Ismail Ben Mosbah Jean-Charles Duclos-Vallée Anne Dubart-Kupperschmitt Bruno Le Pioufle
author_facet	Manon Boul Nassima Benzoubir Antonietta Messina Rasta Ghasemi Ismail Ben Mosbah Jean-Charles Duclos-Vallée Anne Dubart-Kupperschmitt Bruno Le Pioufle
author_sort	Manon Boul
title	A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation
title_short	A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation
title_full	A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation
title_fullStr	A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation
title_full_unstemmed	A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation
title_sort	versatile microfluidic tool for the 3d culture of heparg cells seeded at various stages of differentiation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2efdb3a409f7419390cda61f1cba0a51
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A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation

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