Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells

Abstract The development of chemoresistance and inability in elimination of cancer stem cells are among the key limitations of cancer chemotherapy. Novel molecular therapeutic strategies able to overcome such limitations are urgently needed for future effective management of cancer. In this report,...

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Autores principales:	Hadi AlShamaileh, Tao Wang, Dongxi Xiang, Wang Yin, Phuong Ha-Lien Tran, Roberto A. Barrero, Pei-Zhuo Zhang, Yong Li, Lingxue Kong, Ke Liu, Shu-Feng Zhou, Yingchun Hou, Sarah Shigdar, Wei Duan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f03ea427ce84c7a8ed4ee36eb475dc2
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spelling	oai:doaj.org-article:2f03ea427ce84c7a8ed4ee36eb475dc22021-12-02T11:40:14ZAptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells10.1038/s41598-017-05859-z2045-2322https://doaj.org/article/2f03ea427ce84c7a8ed4ee36eb475dc22017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05859-zhttps://doaj.org/toc/2045-2322Abstract The development of chemoresistance and inability in elimination of cancer stem cells are among the key limitations of cancer chemotherapy. Novel molecular therapeutic strategies able to overcome such limitations are urgently needed for future effective management of cancer. In this report, we show that EpCAM-aptamer-guided survivin RNAi effectively downregulated survivin both in colorectal cancer cells in vitro and in a mouse xenograft model for colorectal cancer. When combined with the conventional chemotherapeutic agents, the aptamer-guided survivin RNAi was able to enhance the sensitivity towards 5-FU or oxaliplatin in colorectal cancer stem cells, increase apoptosis, inhibit tumour growth and improve the overall survival of mice bearing xenograft colorectal cancer. Our results indicate that survivin is one of the key players responsible for the innate chemoresistance of colorectal cancer stem cells. Thus, aptamer-mediated targeting of survivin in cancer stem cells in combination with chemotherapeutic drugs constitutes a new avenue to improve treatment outcome in oncologic clinics.Hadi AlShamailehTao WangDongxi XiangWang YinPhuong Ha-Lien TranRoberto A. BarreroPei-Zhuo ZhangYong LiLingxue KongKe LiuShu-Feng ZhouYingchun HouSarah ShigdarWei DuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hadi AlShamaileh Tao Wang Dongxi Xiang Wang Yin Phuong Ha-Lien Tran Roberto A. Barrero Pei-Zhuo Zhang Yong Li Lingxue Kong Ke Liu Shu-Feng Zhou Yingchun Hou Sarah Shigdar Wei Duan Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells
description	Abstract The development of chemoresistance and inability in elimination of cancer stem cells are among the key limitations of cancer chemotherapy. Novel molecular therapeutic strategies able to overcome such limitations are urgently needed for future effective management of cancer. In this report, we show that EpCAM-aptamer-guided survivin RNAi effectively downregulated survivin both in colorectal cancer cells in vitro and in a mouse xenograft model for colorectal cancer. When combined with the conventional chemotherapeutic agents, the aptamer-guided survivin RNAi was able to enhance the sensitivity towards 5-FU or oxaliplatin in colorectal cancer stem cells, increase apoptosis, inhibit tumour growth and improve the overall survival of mice bearing xenograft colorectal cancer. Our results indicate that survivin is one of the key players responsible for the innate chemoresistance of colorectal cancer stem cells. Thus, aptamer-mediated targeting of survivin in cancer stem cells in combination with chemotherapeutic drugs constitutes a new avenue to improve treatment outcome in oncologic clinics.
format	article
author	Hadi AlShamaileh Tao Wang Dongxi Xiang Wang Yin Phuong Ha-Lien Tran Roberto A. Barrero Pei-Zhuo Zhang Yong Li Lingxue Kong Ke Liu Shu-Feng Zhou Yingchun Hou Sarah Shigdar Wei Duan
author_facet	Hadi AlShamaileh Tao Wang Dongxi Xiang Wang Yin Phuong Ha-Lien Tran Roberto A. Barrero Pei-Zhuo Zhang Yong Li Lingxue Kong Ke Liu Shu-Feng Zhou Yingchun Hou Sarah Shigdar Wei Duan
author_sort	Hadi AlShamaileh
title	Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells
title_short	Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells
title_full	Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells
title_fullStr	Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells
title_full_unstemmed	Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells
title_sort	aptamer-mediated survivin rnai enables 5-fluorouracil to eliminate colorectal cancer stem cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2f03ea427ce84c7a8ed4ee36eb475dc2
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Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells

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