The Two-Way Interaction between the Molecules That Cause Vaginal Malodour and Lactobacilli: An Opportunity for Probiotics

Vaginal malodour is a sign of dysbiosis. The biogenic amines (BAs) cadaverine, putrescine and tyramine are known to be causative compounds. Recent reports suggest these compounds produced by pathogens might have a role beyond causing malodour; namely inhibiting the growth of lactobacilli bacteria th...

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Autores principales:	Scarlett Puebla-Barragan, Polycronis Paul Akouris, Kait F. Al, Charles Carr, Britney Lamb, Mark Sumarah, Charlotte van der Veer, Remco Kort, Jeremy Burton, Gregor Reid
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	vaginal malodour dysbiosis probiotics lactobacilli vaginal microbiota biogenic amines Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2f049d077c8948569c654c152f6a9965
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spelling	oai:doaj.org-article:2f049d077c8948569c654c152f6a99652021-11-25T17:54:59ZThe Two-Way Interaction between the Molecules That Cause Vaginal Malodour and Lactobacilli: An Opportunity for Probiotics10.3390/ijms2222122791422-00671661-6596https://doaj.org/article/2f049d077c8948569c654c152f6a99652021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12279https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Vaginal malodour is a sign of dysbiosis. The biogenic amines (BAs) cadaverine, putrescine and tyramine are known to be causative compounds. Recent reports suggest these compounds produced by pathogens might have a role beyond causing malodour; namely inhibiting the growth of lactobacilli bacteria that are crucial in the maintenance of vaginal homeostasis. The aim of this study was to identify whether certain lactobacilli strains could reduce BAs and to evaluate how <i>Lactobacillus</i> species were affected by these compounds. Using LC–MS and HPLC-UV, five <i>Lactobacillus crispatus</i> strains were identified as being capable of significantly reducing BAs from the media under in vitro conditions. Through 16S rRNA gene sequencing of vaginal swabs exposed to Bas, cadaverine was found to reduce the relative abundance of lactobacilli. When <i>L. crispatus</i> was exposed to media supplemented with BAs with an HCl adjusted lower pH, its growth was enhanced, demonstrating the relevance of the maintenance of an acidic vaginal environment. If strains are to be developed for probiotic application to alleviate bacterial vaginosis and other conditions affecting large numbers of women worldwide, their ability to adapt to Bas and regulate pH should be part of the experimentation.Scarlett Puebla-BarraganPolycronis Paul AkourisKait F. AlCharles CarrBritney LambMark SumarahCharlotte van der VeerRemco KortJeremy BurtonGregor ReidMDPI AGarticlevaginal malodourdysbiosisprobioticslactobacillivaginal microbiotabiogenic aminesBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12279, p 12279 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vaginal malodour dysbiosis probiotics lactobacilli vaginal microbiota biogenic amines Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	vaginal malodour dysbiosis probiotics lactobacilli vaginal microbiota biogenic amines Biology (General) QH301-705.5 Chemistry QD1-999 Scarlett Puebla-Barragan Polycronis Paul Akouris Kait F. Al Charles Carr Britney Lamb Mark Sumarah Charlotte van der Veer Remco Kort Jeremy Burton Gregor Reid The Two-Way Interaction between the Molecules That Cause Vaginal Malodour and Lactobacilli: An Opportunity for Probiotics
description	Vaginal malodour is a sign of dysbiosis. The biogenic amines (BAs) cadaverine, putrescine and tyramine are known to be causative compounds. Recent reports suggest these compounds produced by pathogens might have a role beyond causing malodour; namely inhibiting the growth of lactobacilli bacteria that are crucial in the maintenance of vaginal homeostasis. The aim of this study was to identify whether certain lactobacilli strains could reduce BAs and to evaluate how <i>Lactobacillus</i> species were affected by these compounds. Using LC–MS and HPLC-UV, five <i>Lactobacillus crispatus</i> strains were identified as being capable of significantly reducing BAs from the media under in vitro conditions. Through 16S rRNA gene sequencing of vaginal swabs exposed to Bas, cadaverine was found to reduce the relative abundance of lactobacilli. When <i>L. crispatus</i> was exposed to media supplemented with BAs with an HCl adjusted lower pH, its growth was enhanced, demonstrating the relevance of the maintenance of an acidic vaginal environment. If strains are to be developed for probiotic application to alleviate bacterial vaginosis and other conditions affecting large numbers of women worldwide, their ability to adapt to Bas and regulate pH should be part of the experimentation.
format	article
author	Scarlett Puebla-Barragan Polycronis Paul Akouris Kait F. Al Charles Carr Britney Lamb Mark Sumarah Charlotte van der Veer Remco Kort Jeremy Burton Gregor Reid
author_facet	Scarlett Puebla-Barragan Polycronis Paul Akouris Kait F. Al Charles Carr Britney Lamb Mark Sumarah Charlotte van der Veer Remco Kort Jeremy Burton Gregor Reid
author_sort	Scarlett Puebla-Barragan
title	The Two-Way Interaction between the Molecules That Cause Vaginal Malodour and Lactobacilli: An Opportunity for Probiotics
title_short	The Two-Way Interaction between the Molecules That Cause Vaginal Malodour and Lactobacilli: An Opportunity for Probiotics
title_full	The Two-Way Interaction between the Molecules That Cause Vaginal Malodour and Lactobacilli: An Opportunity for Probiotics
title_fullStr	The Two-Way Interaction between the Molecules That Cause Vaginal Malodour and Lactobacilli: An Opportunity for Probiotics
title_full_unstemmed	The Two-Way Interaction between the Molecules That Cause Vaginal Malodour and Lactobacilli: An Opportunity for Probiotics
title_sort	two-way interaction between the molecules that cause vaginal malodour and lactobacilli: an opportunity for probiotics
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2f049d077c8948569c654c152f6a9965
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The Two-Way Interaction between the Molecules That Cause Vaginal Malodour and Lactobacilli: An Opportunity for Probiotics

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