Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy

Ying-Zheng Zhao,1,2 Ming Zhang,1 Xin-Qiao Tian,3 Lei Zheng,4 Cui-Tao Lu1,2 1College of Pharmaceutical Sciences, Wenzhou Medical University, 2Department of Ultrasonography, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 3Department of Ultrasonic Diagnosis, Henan Pro...

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Autores principales:	Zhao YZ, Zhang M, Tian XQ, Zheng L, Lu CT
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	diabetic cardiomyopathy prevention bFGF-load liposome ultrasound-targeted microbubble destruction technique Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2f089f947c8a4516ae89f7b59ffe48df
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spelling	oai:doaj.org-article:2f089f947c8a4516ae89f7b59ffe48df2021-12-02T01:45:10ZUsing basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy1178-2013https://doaj.org/article/2f089f947c8a4516ae89f7b59ffe48df2016-02-01T00:00:00Zhttps://www.dovepress.com/using-basic-fibroblast-growth-factor-nanoliposome-combined-with-ultras-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ying-Zheng Zhao,1,2 Ming Zhang,1 Xin-Qiao Tian,3 Lei Zheng,4 Cui-Tao Lu1,2 1College of Pharmaceutical Sciences, Wenzhou Medical University, 2Department of Ultrasonography, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 3Department of Ultrasonic Diagnosis, Henan Provincial People’s Hospital, Zhengzhou City, Henan, 4Department of Ultrasonography, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China Abstract: Basic fibroblast growth factor (bFGF)-loaded liposome (bFGF-lip) combined with ultrasound-targeted microbubble destruction (UTMD) technique was investigated to prevent diabetic cardiomyopathy (DCM). Cardiac function and myocardial ultrastructure were assessed. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) staining, immunohistochemistry staining, and Western blot assay were used to investigate the signal pathway underlying the expression of bFGF in DCM treatment. From Mason staining and TUNEL staining, bFGF-lip + UTMD group showed significant differences from the diabetes group and other groups treated with bFGF or bFGF-lip. The diabetes group showed similar results (myocardial capillary density, collagen volume fraction, and cardiac myocyte apoptosis index) to other bFGF treatment groups. Indexes from transthoracic echocardiography and hemodynamic evaluation also proved the same conclusion. These results confirmed that the abnormalities including diastolic dysfunctions, myocardial fibrosis, and metabolic disturbances could be suppressed by the different extents of twice-weekly bFGF treatments for 12 consecutive weeks (free bFGF or bFGF-lip +/- UTMD), with the strongest improvements observed in the bFGF-lip + UTMD group. The group combining bFGF-lip with UTMD demonstrated the highest level of bFGF expression among all the groups. The bFGF activated the PI3K/AKT signal pathway, causing the reduction of myocardial cell apoptosis and increase of microvascular density. This strategy using bFGF-lip and UTMD is a potential strategy in early intervention of DCM in diabetes. Keywords: diabetic cardiomyopathy, prevention, bFGF-loaded liposome, targeted deliveryZhao YZZhang MTian XQZheng LLu CTDove Medical Pressarticlediabetic cardiomyopathypreventionbFGF-load liposomeultrasound-targeted microbubble destruction techniqueMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 675-686 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetic cardiomyopathy prevention bFGF-load liposome ultrasound-targeted microbubble destruction technique Medicine (General) R5-920
spellingShingle	diabetic cardiomyopathy prevention bFGF-load liposome ultrasound-targeted microbubble destruction technique Medicine (General) R5-920 Zhao YZ Zhang M Tian XQ Zheng L Lu CT Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy
description	Ying-Zheng Zhao,1,2 Ming Zhang,1 Xin-Qiao Tian,3 Lei Zheng,4 Cui-Tao Lu1,2 1College of Pharmaceutical Sciences, Wenzhou Medical University, 2Department of Ultrasonography, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 3Department of Ultrasonic Diagnosis, Henan Provincial People’s Hospital, Zhengzhou City, Henan, 4Department of Ultrasonography, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China Abstract: Basic fibroblast growth factor (bFGF)-loaded liposome (bFGF-lip) combined with ultrasound-targeted microbubble destruction (UTMD) technique was investigated to prevent diabetic cardiomyopathy (DCM). Cardiac function and myocardial ultrastructure were assessed. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) staining, immunohistochemistry staining, and Western blot assay were used to investigate the signal pathway underlying the expression of bFGF in DCM treatment. From Mason staining and TUNEL staining, bFGF-lip + UTMD group showed significant differences from the diabetes group and other groups treated with bFGF or bFGF-lip. The diabetes group showed similar results (myocardial capillary density, collagen volume fraction, and cardiac myocyte apoptosis index) to other bFGF treatment groups. Indexes from transthoracic echocardiography and hemodynamic evaluation also proved the same conclusion. These results confirmed that the abnormalities including diastolic dysfunctions, myocardial fibrosis, and metabolic disturbances could be suppressed by the different extents of twice-weekly bFGF treatments for 12 consecutive weeks (free bFGF or bFGF-lip +/- UTMD), with the strongest improvements observed in the bFGF-lip + UTMD group. The group combining bFGF-lip with UTMD demonstrated the highest level of bFGF expression among all the groups. The bFGF activated the PI3K/AKT signal pathway, causing the reduction of myocardial cell apoptosis and increase of microvascular density. This strategy using bFGF-lip and UTMD is a potential strategy in early intervention of DCM in diabetes. Keywords: diabetic cardiomyopathy, prevention, bFGF-loaded liposome, targeted delivery
format	article
author	Zhao YZ Zhang M Tian XQ Zheng L Lu CT
author_facet	Zhao YZ Zhang M Tian XQ Zheng L Lu CT
author_sort	Zhao YZ
title	Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy
title_short	Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy
title_full	Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy
title_fullStr	Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy
title_full_unstemmed	Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy
title_sort	using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/2f089f947c8a4516ae89f7b59ffe48df
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Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy

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