Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue

Summary: Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach impr...

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Auteurs principaux:	Tarlan Vatan, Jacqueline A. Minehart, Chenghang Zhang, Vatsal Agarwal, Jerry Yang, Colenso M. Speer
Format:	article
Langue:	EN
Publié:	Elsevier 2021
Sujets:	Cell Biology Microscopy Neuroscience Science (General) Q1-390
Accès en ligne:	https://doaj.org/article/2f090f4fe5694afe9a6c8349d4c2a85e
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spelling	oai:doaj.org-article:2f090f4fe5694afe9a6c8349d4c2a85e2021-11-26T04:40:36ZVolumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue2666-166710.1016/j.xpro.2021.100971https://doaj.org/article/2f090f4fe5694afe9a6c8349d4c2a85e2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006778https://doaj.org/toc/2666-1667Summary: Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach improves 3D STORM image quality by reducing tissue scattering, photobleaching, and optical distortions associated with deep imaging. This approach can be extended for use on other tissue types enabling life scientists to perform volumetric super-resolution imaging in diverse biological models.For complete details on the use and execution of this protocol, please refer to Sigal et al. (2015).Tarlan VatanJacqueline A. MinehartChenghang ZhangVatsal AgarwalJerry YangColenso M. SpeerElsevierarticleCell BiologyMicroscopyNeuroscienceScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100971- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Microscopy Neuroscience Science (General) Q1-390
spellingShingle	Cell Biology Microscopy Neuroscience Science (General) Q1-390 Tarlan Vatan Jacqueline A. Minehart Chenghang Zhang Vatsal Agarwal Jerry Yang Colenso M. Speer Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
description	Summary: Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach improves 3D STORM image quality by reducing tissue scattering, photobleaching, and optical distortions associated with deep imaging. This approach can be extended for use on other tissue types enabling life scientists to perform volumetric super-resolution imaging in diverse biological models.For complete details on the use and execution of this protocol, please refer to Sigal et al. (2015).
format	article
author	Tarlan Vatan Jacqueline A. Minehart Chenghang Zhang Vatsal Agarwal Jerry Yang Colenso M. Speer
author_facet	Tarlan Vatan Jacqueline A. Minehart Chenghang Zhang Vatsal Agarwal Jerry Yang Colenso M. Speer
author_sort	Tarlan Vatan
title	Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
title_short	Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
title_full	Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
title_fullStr	Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
title_full_unstemmed	Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
title_sort	volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2f090f4fe5694afe9a6c8349d4c2a85e
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Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue

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