Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue

Summary: Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach impr...

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Auteurs principaux: Tarlan Vatan, Jacqueline A. Minehart, Chenghang Zhang, Vatsal Agarwal, Jerry Yang, Colenso M. Speer
Format: article
Langue:EN
Publié: Elsevier 2021
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Accès en ligne:https://doaj.org/article/2f090f4fe5694afe9a6c8349d4c2a85e
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spelling oai:doaj.org-article:2f090f4fe5694afe9a6c8349d4c2a85e2021-11-26T04:40:36ZVolumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue2666-166710.1016/j.xpro.2021.100971https://doaj.org/article/2f090f4fe5694afe9a6c8349d4c2a85e2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006778https://doaj.org/toc/2666-1667Summary: Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach improves 3D STORM image quality by reducing tissue scattering, photobleaching, and optical distortions associated with deep imaging. This approach can be extended for use on other tissue types enabling life scientists to perform volumetric super-resolution imaging in diverse biological models.For complete details on the use and execution of this protocol, please refer to Sigal et al. (2015).Tarlan VatanJacqueline A. MinehartChenghang ZhangVatsal AgarwalJerry YangColenso M. SpeerElsevierarticleCell BiologyMicroscopyNeuroscienceScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100971- (2021)
institution DOAJ
collection DOAJ
language EN
topic Cell Biology
Microscopy
Neuroscience
Science (General)
Q1-390
spellingShingle Cell Biology
Microscopy
Neuroscience
Science (General)
Q1-390
Tarlan Vatan
Jacqueline A. Minehart
Chenghang Zhang
Vatsal Agarwal
Jerry Yang
Colenso M. Speer
Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
description Summary: Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach improves 3D STORM image quality by reducing tissue scattering, photobleaching, and optical distortions associated with deep imaging. This approach can be extended for use on other tissue types enabling life scientists to perform volumetric super-resolution imaging in diverse biological models.For complete details on the use and execution of this protocol, please refer to Sigal et al. (2015).
format article
author Tarlan Vatan
Jacqueline A. Minehart
Chenghang Zhang
Vatsal Agarwal
Jerry Yang
Colenso M. Speer
author_facet Tarlan Vatan
Jacqueline A. Minehart
Chenghang Zhang
Vatsal Agarwal
Jerry Yang
Colenso M. Speer
author_sort Tarlan Vatan
title Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
title_short Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
title_full Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
title_fullStr Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
title_full_unstemmed Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
title_sort volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue
publisher Elsevier
publishDate 2021
url https://doaj.org/article/2f090f4fe5694afe9a6c8349d4c2a85e
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AT jacquelineaminehart volumetricsuperresolutionimagingbyserialultrasectioningandstochasticopticalreconstructionmicroscopyinmouseneuraltissue
AT chenghangzhang volumetricsuperresolutionimagingbyserialultrasectioningandstochasticopticalreconstructionmicroscopyinmouseneuraltissue
AT vatsalagarwal volumetricsuperresolutionimagingbyserialultrasectioningandstochasticopticalreconstructionmicroscopyinmouseneuraltissue
AT jerryyang volumetricsuperresolutionimagingbyserialultrasectioningandstochasticopticalreconstructionmicroscopyinmouseneuraltissue
AT colensomspeer volumetricsuperresolutionimagingbyserialultrasectioningandstochasticopticalreconstructionmicroscopyinmouseneuraltissue
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