Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue

Summary: Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach impr...

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Autores principales: Tarlan Vatan, Jacqueline A. Minehart, Chenghang Zhang, Vatsal Agarwal, Jerry Yang, Colenso M. Speer
Formato: article
Lenguaje:EN
Publicado: Elsevier 2021
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Acceso en línea:https://doaj.org/article/2f090f4fe5694afe9a6c8349d4c2a85e
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