Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue

Summary: Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach impr...

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Auteurs principaux: Tarlan Vatan, Jacqueline A. Minehart, Chenghang Zhang, Vatsal Agarwal, Jerry Yang, Colenso M. Speer
Format: article
Langue:EN
Publié: Elsevier 2021
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Accès en ligne:https://doaj.org/article/2f090f4fe5694afe9a6c8349d4c2a85e
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