Spatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function

Abstract Tight junction complexes are involved in the establishment and maintenance of cell polarity and the regulation of signalling pathways, controlling biological processes such as cell differentiation and cell proliferation. MarvelD3 is a tight junction protein expressed in adult epithelial and...

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Autores principales:	Charlotte Heymans, Ophélie Delcorte, Catherine Spourquet, Mylah Villacorte-Tabelin, Sébastien Dupasquier, Younes Achouri, Siam Mahibullah, Pascale Lemoine, Maria S. Balda, Karl Matter, Christophe E. Pierreux
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f0bd55705b64e16964177485120a440
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spelling	oai:doaj.org-article:2f0bd55705b64e16964177485120a4402021-12-02T15:33:14ZSpatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function10.1038/s41598-021-93654-22045-2322https://doaj.org/article/2f0bd55705b64e16964177485120a4402021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93654-2https://doaj.org/toc/2045-2322Abstract Tight junction complexes are involved in the establishment and maintenance of cell polarity and the regulation of signalling pathways, controlling biological processes such as cell differentiation and cell proliferation. MarvelD3 is a tight junction protein expressed in adult epithelial and endothelial cells. In Xenopus laevis, MarvelD3 morphants present differentiation defects of several ectodermal derivatives. In vitro experiments further revealed that MarvelD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behaviour and survival. In this work, we found that MarvelD3 is expressed from early developmental stages in the exocrine and endocrine compartments of the pancreas, as well as in endothelial cells of this organ. We thoroughly characterized MarvelD3 expression pattern in developing pancreas and evaluated its function by genetic ablation. Surprisingly, inactivation of MarvelD3 in mice did not alter development and differentiation of the pancreatic tissue. Moreover, tight junction formation and organization, cell polarization, and activity of the JNK-pathway were not impacted by the deletion of MarvelD3.Charlotte HeymansOphélie DelcorteCatherine SpourquetMylah Villacorte-TabelinSébastien DupasquierYounes AchouriSiam MahibullahPascale LemoineMaria S. BaldaKarl MatterChristophe E. PierreuxNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charlotte Heymans Ophélie Delcorte Catherine Spourquet Mylah Villacorte-Tabelin Sébastien Dupasquier Younes Achouri Siam Mahibullah Pascale Lemoine Maria S. Balda Karl Matter Christophe E. Pierreux Spatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function
description	Abstract Tight junction complexes are involved in the establishment and maintenance of cell polarity and the regulation of signalling pathways, controlling biological processes such as cell differentiation and cell proliferation. MarvelD3 is a tight junction protein expressed in adult epithelial and endothelial cells. In Xenopus laevis, MarvelD3 morphants present differentiation defects of several ectodermal derivatives. In vitro experiments further revealed that MarvelD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behaviour and survival. In this work, we found that MarvelD3 is expressed from early developmental stages in the exocrine and endocrine compartments of the pancreas, as well as in endothelial cells of this organ. We thoroughly characterized MarvelD3 expression pattern in developing pancreas and evaluated its function by genetic ablation. Surprisingly, inactivation of MarvelD3 in mice did not alter development and differentiation of the pancreatic tissue. Moreover, tight junction formation and organization, cell polarization, and activity of the JNK-pathway were not impacted by the deletion of MarvelD3.
format	article
author	Charlotte Heymans Ophélie Delcorte Catherine Spourquet Mylah Villacorte-Tabelin Sébastien Dupasquier Younes Achouri Siam Mahibullah Pascale Lemoine Maria S. Balda Karl Matter Christophe E. Pierreux
author_facet	Charlotte Heymans Ophélie Delcorte Catherine Spourquet Mylah Villacorte-Tabelin Sébastien Dupasquier Younes Achouri Siam Mahibullah Pascale Lemoine Maria S. Balda Karl Matter Christophe E. Pierreux
author_sort	Charlotte Heymans
title	Spatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function
title_short	Spatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function
title_full	Spatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function
title_fullStr	Spatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function
title_full_unstemmed	Spatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function
title_sort	spatio-temporal expression pattern and role of the tight junction protein marveld3 in pancreas development and function
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2f0bd55705b64e16964177485120a440
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Spatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function

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