A microfabricated multi-compartment device for neuron and Schwann cell differentiation

Abstract Understanding the complex communication between different cell populations and their interaction with the microenvironment in the central and peripheral nervous systems is fundamental in neuroscience research. The development of appropriate in vitro approaches and tools, able to selectively...

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Autores principales:	Eleonora De Vitis, Velia La Pesa, Francesca Gervaso, Alessandro Romano, Angelo Quattrini, Giuseppe Gigli, Lorenzo Moroni, Alessandro Polini
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f10b8d2d78c4303b2ba0c0c109f9291
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spelling	oai:doaj.org-article:2f10b8d2d78c4303b2ba0c0c109f92912021-12-02T18:17:54ZA microfabricated multi-compartment device for neuron and Schwann cell differentiation10.1038/s41598-021-86300-42045-2322https://doaj.org/article/2f10b8d2d78c4303b2ba0c0c109f92912021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86300-4https://doaj.org/toc/2045-2322Abstract Understanding the complex communication between different cell populations and their interaction with the microenvironment in the central and peripheral nervous systems is fundamental in neuroscience research. The development of appropriate in vitro approaches and tools, able to selectively analyze and/or probe specific cells and cell portions (e.g., axons and cell bodies in neurons), driving their differentiation into specific cell phenotypes, has become therefore crucial in this direction. Here we report a multi-compartment microfluidic device where up to three different cell populations can be cultured in a fluidically independent circuit. The device allows cell migration across the compartments and their differentiation. We showed that an accurate choice of the device geometrical features and cell culture parameters allows to (1) maximize cell adhesion and proliferation of neuron-like human cells (SH-SY5Y cells), (2) control the inter-compartment cell migration of neuron and Schwann cells, (3) perform long-term cell culture studies in which both SH-SY5Y cells and primary rat Schwann cells can be differentiated towards specific phenotypes. These results can lead to a plethora of in vitro co-culture studies in the neuroscience research field, where tuning and investigating cell–cell and cell–microenvironment interactions are essential.Eleonora De VitisVelia La PesaFrancesca GervasoAlessandro RomanoAngelo QuattriniGiuseppe GigliLorenzo MoroniAlessandro PoliniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eleonora De Vitis Velia La Pesa Francesca Gervaso Alessandro Romano Angelo Quattrini Giuseppe Gigli Lorenzo Moroni Alessandro Polini A microfabricated multi-compartment device for neuron and Schwann cell differentiation
description	Abstract Understanding the complex communication between different cell populations and their interaction with the microenvironment in the central and peripheral nervous systems is fundamental in neuroscience research. The development of appropriate in vitro approaches and tools, able to selectively analyze and/or probe specific cells and cell portions (e.g., axons and cell bodies in neurons), driving their differentiation into specific cell phenotypes, has become therefore crucial in this direction. Here we report a multi-compartment microfluidic device where up to three different cell populations can be cultured in a fluidically independent circuit. The device allows cell migration across the compartments and their differentiation. We showed that an accurate choice of the device geometrical features and cell culture parameters allows to (1) maximize cell adhesion and proliferation of neuron-like human cells (SH-SY5Y cells), (2) control the inter-compartment cell migration of neuron and Schwann cells, (3) perform long-term cell culture studies in which both SH-SY5Y cells and primary rat Schwann cells can be differentiated towards specific phenotypes. These results can lead to a plethora of in vitro co-culture studies in the neuroscience research field, where tuning and investigating cell–cell and cell–microenvironment interactions are essential.
format	article
author	Eleonora De Vitis Velia La Pesa Francesca Gervaso Alessandro Romano Angelo Quattrini Giuseppe Gigli Lorenzo Moroni Alessandro Polini
author_facet	Eleonora De Vitis Velia La Pesa Francesca Gervaso Alessandro Romano Angelo Quattrini Giuseppe Gigli Lorenzo Moroni Alessandro Polini
author_sort	Eleonora De Vitis
title	A microfabricated multi-compartment device for neuron and Schwann cell differentiation
title_short	A microfabricated multi-compartment device for neuron and Schwann cell differentiation
title_full	A microfabricated multi-compartment device for neuron and Schwann cell differentiation
title_fullStr	A microfabricated multi-compartment device for neuron and Schwann cell differentiation
title_full_unstemmed	A microfabricated multi-compartment device for neuron and Schwann cell differentiation
title_sort	microfabricated multi-compartment device for neuron and schwann cell differentiation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2f10b8d2d78c4303b2ba0c0c109f9291
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A microfabricated multi-compartment device for neuron and Schwann cell differentiation

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