Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance.

Epigallocatechin gallate (EGCG) is a major polyphenol in green tea that has been shown to have anti-inflammatory, anti-cancer, anti-steatotic effects on the liver. Autophagy also mediates similar effects; however, it is not currently known whether EGCG can regulate hepatic autophagy. Here, we show t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jin Zhou, Benjamin Livingston Farah, Rohit Anthony Sinha, Yajun Wu, Brijesh Kumar Singh, Boon-Huat Bay, Chung S Yang, Paul Michael Yen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f171db8aac94feba866b24eb5e08ac2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2f171db8aac94feba866b24eb5e08ac2
record_format	dspace
spelling	oai:doaj.org-article:2f171db8aac94feba866b24eb5e08ac22021-11-18T08:35:04ZEpigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance.1932-620310.1371/journal.pone.0087161https://doaj.org/article/2f171db8aac94feba866b24eb5e08ac22014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24489859/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Epigallocatechin gallate (EGCG) is a major polyphenol in green tea that has been shown to have anti-inflammatory, anti-cancer, anti-steatotic effects on the liver. Autophagy also mediates similar effects; however, it is not currently known whether EGCG can regulate hepatic autophagy. Here, we show that EGCG increases hepatic autophagy by promoting the formation of autophagosomes, increasing lysosomal acidification, and stimulating autophagic flux in hepatic cells and in vivo. EGCG also increases phosphorylation of AMPK, one of the major regulators of autophagy. Importantly, siRNA knockdown of AMPK abrogated autophagy induced by EGCG. Interestingly, we observed lipid droplet within autophagosomes and autolysosomes and increased lipid clearance by EGCG, suggesting it promotes lipid metabolism by increasing autophagy. In mice fed with high-fat/western style diet (HFW; 60% energy as fat, reduced levels of calcium, vitamin D3, choline, folate, and fiber), EGCG treatment reduces hepatosteatosis and concomitantly increases autophagy. In summary, we have used genetic and pharmacological approaches to demonstrate EGCG induction of hepatic autophagy, and this may contribute to its beneficial effects in reducing hepatosteatosis and potentially some other pathological liver conditions.Jin ZhouBenjamin Livingston FarahRohit Anthony SinhaYajun WuBrijesh Kumar SinghBoon-Huat BayChung S YangPaul Michael YenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e87161 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jin Zhou Benjamin Livingston Farah Rohit Anthony Sinha Yajun Wu Brijesh Kumar Singh Boon-Huat Bay Chung S Yang Paul Michael Yen Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance.
description	Epigallocatechin gallate (EGCG) is a major polyphenol in green tea that has been shown to have anti-inflammatory, anti-cancer, anti-steatotic effects on the liver. Autophagy also mediates similar effects; however, it is not currently known whether EGCG can regulate hepatic autophagy. Here, we show that EGCG increases hepatic autophagy by promoting the formation of autophagosomes, increasing lysosomal acidification, and stimulating autophagic flux in hepatic cells and in vivo. EGCG also increases phosphorylation of AMPK, one of the major regulators of autophagy. Importantly, siRNA knockdown of AMPK abrogated autophagy induced by EGCG. Interestingly, we observed lipid droplet within autophagosomes and autolysosomes and increased lipid clearance by EGCG, suggesting it promotes lipid metabolism by increasing autophagy. In mice fed with high-fat/western style diet (HFW; 60% energy as fat, reduced levels of calcium, vitamin D3, choline, folate, and fiber), EGCG treatment reduces hepatosteatosis and concomitantly increases autophagy. In summary, we have used genetic and pharmacological approaches to demonstrate EGCG induction of hepatic autophagy, and this may contribute to its beneficial effects in reducing hepatosteatosis and potentially some other pathological liver conditions.
format	article
author	Jin Zhou Benjamin Livingston Farah Rohit Anthony Sinha Yajun Wu Brijesh Kumar Singh Boon-Huat Bay Chung S Yang Paul Michael Yen
author_facet	Jin Zhou Benjamin Livingston Farah Rohit Anthony Sinha Yajun Wu Brijesh Kumar Singh Boon-Huat Bay Chung S Yang Paul Michael Yen
author_sort	Jin Zhou
title	Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance.
title_short	Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance.
title_full	Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance.
title_fullStr	Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance.
title_full_unstemmed	Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance.
title_sort	epigallocatechin-3-gallate (egcg), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/2f171db8aac94feba866b24eb5e08ac2
work_keys_str_mv	AT jinzhou epigallocatechin3gallateegcgagreenteapolyphenolstimulateshepaticautophagyandlipidclearance AT benjaminlivingstonfarah epigallocatechin3gallateegcgagreenteapolyphenolstimulateshepaticautophagyandlipidclearance AT rohitanthonysinha epigallocatechin3gallateegcgagreenteapolyphenolstimulateshepaticautophagyandlipidclearance AT yajunwu epigallocatechin3gallateegcgagreenteapolyphenolstimulateshepaticautophagyandlipidclearance AT brijeshkumarsingh epigallocatechin3gallateegcgagreenteapolyphenolstimulateshepaticautophagyandlipidclearance AT boonhuatbay epigallocatechin3gallateegcgagreenteapolyphenolstimulateshepaticautophagyandlipidclearance AT chungsyang epigallocatechin3gallateegcgagreenteapolyphenolstimulateshepaticautophagyandlipidclearance AT paulmichaelyen epigallocatechin3gallateegcgagreenteapolyphenolstimulateshepaticautophagyandlipidclearance
_version_	1718421611059609600

Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance.

Ejemplares similares