Roles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro

Abstract Transforming growth factor β (TGFβ) and fibroblast growth factor (FGF) signaling pathways play important roles in the proliferation and differentiation of lens epithelial cells (LECs) during development. Low dosage bFGF promotes cell proliferation while high dosage induces differentiation....

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Autores principales:	Dong Wang, Eddie Wang, Kelsey Liu, Chun-hong Xia, Song Li, Xiaohua Gong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f183bf36d2643e7a4bf43af4296f009
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spelling	oai:doaj.org-article:2f183bf36d2643e7a4bf43af4296f0092021-12-02T15:06:01ZRoles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro10.1038/s41598-017-07619-52045-2322https://doaj.org/article/2f183bf36d2643e7a4bf43af4296f0092017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07619-5https://doaj.org/toc/2045-2322Abstract Transforming growth factor β (TGFβ) and fibroblast growth factor (FGF) signaling pathways play important roles in the proliferation and differentiation of lens epithelial cells (LECs) during development. Low dosage bFGF promotes cell proliferation while high dosage induces differentiation. TGFβ signaling regulates LEC proliferation and differentiation as well, but also promotes epithelial-mesenchymal transitions that lead to cataracts. Thus far, it has been difficult to recapitulate the features of germinative LECs in vitro. Here, we have established a LEC culture protocol that uses SB431542 (SB) compound to inhibit TGFβ/Smad activation, and found that SB treatment promoted mouse LEC proliferation, maintained LECs’ morphology and distinct markers including N-cadherin, c-Maf, Prox1, and αA-, αB-, and β-crystallins. In contrast, low-dosage bFGF was unable to sustain those markers and, combined with SB, altered LECs’ morphology and β-crystallin expression. We further found that Matrigel substrate coatings greatly increased cell proliferation and uniquely affected β-crystallin expression. Cultured LECs retained the ability to differentiate into γ-crystallin-positive lentoids by high-dosage bFGF treatment. Thus, a suppression of TGFβ/Smad signaling in vitro is critical to maintaining characteristic features of mouse LECs, especially expression of the key transcription factors c-Maf and Prox1.Dong WangEddie WangKelsey LiuChun-hong XiaSong LiXiaohua GongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dong Wang Eddie Wang Kelsey Liu Chun-hong Xia Song Li Xiaohua Gong Roles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro
description	Abstract Transforming growth factor β (TGFβ) and fibroblast growth factor (FGF) signaling pathways play important roles in the proliferation and differentiation of lens epithelial cells (LECs) during development. Low dosage bFGF promotes cell proliferation while high dosage induces differentiation. TGFβ signaling regulates LEC proliferation and differentiation as well, but also promotes epithelial-mesenchymal transitions that lead to cataracts. Thus far, it has been difficult to recapitulate the features of germinative LECs in vitro. Here, we have established a LEC culture protocol that uses SB431542 (SB) compound to inhibit TGFβ/Smad activation, and found that SB treatment promoted mouse LEC proliferation, maintained LECs’ morphology and distinct markers including N-cadherin, c-Maf, Prox1, and αA-, αB-, and β-crystallins. In contrast, low-dosage bFGF was unable to sustain those markers and, combined with SB, altered LECs’ morphology and β-crystallin expression. We further found that Matrigel substrate coatings greatly increased cell proliferation and uniquely affected β-crystallin expression. Cultured LECs retained the ability to differentiate into γ-crystallin-positive lentoids by high-dosage bFGF treatment. Thus, a suppression of TGFβ/Smad signaling in vitro is critical to maintaining characteristic features of mouse LECs, especially expression of the key transcription factors c-Maf and Prox1.
format	article
author	Dong Wang Eddie Wang Kelsey Liu Chun-hong Xia Song Li Xiaohua Gong
author_facet	Dong Wang Eddie Wang Kelsey Liu Chun-hong Xia Song Li Xiaohua Gong
author_sort	Dong Wang
title	Roles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro
title_short	Roles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro
title_full	Roles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro
title_fullStr	Roles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro
title_full_unstemmed	Roles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro
title_sort	roles of tgfβ and fgf signals during growth and differentiation of mouse lens epithelial cell in vitro
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2f183bf36d2643e7a4bf43af4296f009
work_keys_str_mv	AT dongwang rolesoftgfbandfgfsignalsduringgrowthanddifferentiationofmouselensepithelialcellinvitro AT eddiewang rolesoftgfbandfgfsignalsduringgrowthanddifferentiationofmouselensepithelialcellinvitro AT kelseyliu rolesoftgfbandfgfsignalsduringgrowthanddifferentiationofmouselensepithelialcellinvitro AT chunhongxia rolesoftgfbandfgfsignalsduringgrowthanddifferentiationofmouselensepithelialcellinvitro AT songli rolesoftgfbandfgfsignalsduringgrowthanddifferentiationofmouselensepithelialcellinvitro AT xiaohuagong rolesoftgfbandfgfsignalsduringgrowthanddifferentiationofmouselensepithelialcellinvitro
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Roles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro

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