Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic <i>Escherichia coli</i>

Outer membrane proteins are commonly produced by gram-negative bacteria, and they have diverse functions. A subgroup of proteins, which includes OmpA, OmpW and OmpX, is often involved in bacterial pathogenesis. Here we show that OmpA, rather than OmpW or OmpX, contributes to the virulence of enteroh...

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Autores principales:	Hidetada Hirakawa, Kazutomo Suzue, Ayako Takita, Haruyoshi Tomita
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	virulence pathogenicity type III secretion system gastrointestinal infection outer membrane protein enteric pathogen Medicine R
Acceso en línea:	https://doaj.org/article/2f1c3cfb478d49339fe286e97059b89b
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spelling	oai:doaj.org-article:2f1c3cfb478d49339fe286e97059b89b2021-11-25T18:38:43ZRoles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic <i>Escherichia coli</i>10.3390/pathogens101114962076-0817https://doaj.org/article/2f1c3cfb478d49339fe286e97059b89b2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1496https://doaj.org/toc/2076-0817Outer membrane proteins are commonly produced by gram-negative bacteria, and they have diverse functions. A subgroup of proteins, which includes OmpA, OmpW and OmpX, is often involved in bacterial pathogenesis. Here we show that OmpA, rather than OmpW or OmpX, contributes to the virulence of enterohemorrhagic <i>Esch</i><i>erichia coli</i> (EHEC) through its type III secretion system (T3SS). Deletion of <i>ompA</i> decreased secretion of the T3SS proteins EspA and EspB; however, the expression level of the LEE genes that encode a set of T3SS proteins did not decrease. The <i>ompA</i> mutant had less abilities to form A/E lesions in host epithelial cells and lyse human red blood cells than the parent strain. Moreover, the virulence of an <i>ompA</i> mutant of <i>Citrobacter rodentium</i> (traditionally used to estimate T3SS-associated virulence in mice) was attenuated. Mice infected with the <i>ompA</i> mutant survived longer than those infected with the parent strain. Furthermore, mice infected with <i>ompA</i> developed symptoms of diarrhea more slowly than mice infected with the parent strain. Altogether, these results suggest that OmpA sustains the activity of the T3SS and is required for optimal virulence in EHEC. This work expands the roles of outer membrane proteins in bacterial pathogenesis.Hidetada HirakawaKazutomo SuzueAyako TakitaHaruyoshi TomitaMDPI AGarticlevirulencepathogenicitytype III secretion systemgastrointestinal infectionouter membrane proteinenteric pathogenMedicineRENPathogens, Vol 10, Iss 1496, p 1496 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	virulence pathogenicity type III secretion system gastrointestinal infection outer membrane protein enteric pathogen Medicine R
spellingShingle	virulence pathogenicity type III secretion system gastrointestinal infection outer membrane protein enteric pathogen Medicine R Hidetada Hirakawa Kazutomo Suzue Ayako Takita Haruyoshi Tomita Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic <i>Escherichia coli</i>
description	Outer membrane proteins are commonly produced by gram-negative bacteria, and they have diverse functions. A subgroup of proteins, which includes OmpA, OmpW and OmpX, is often involved in bacterial pathogenesis. Here we show that OmpA, rather than OmpW or OmpX, contributes to the virulence of enterohemorrhagic <i>Esch</i><i>erichia coli</i> (EHEC) through its type III secretion system (T3SS). Deletion of <i>ompA</i> decreased secretion of the T3SS proteins EspA and EspB; however, the expression level of the LEE genes that encode a set of T3SS proteins did not decrease. The <i>ompA</i> mutant had less abilities to form A/E lesions in host epithelial cells and lyse human red blood cells than the parent strain. Moreover, the virulence of an <i>ompA</i> mutant of <i>Citrobacter rodentium</i> (traditionally used to estimate T3SS-associated virulence in mice) was attenuated. Mice infected with the <i>ompA</i> mutant survived longer than those infected with the parent strain. Furthermore, mice infected with <i>ompA</i> developed symptoms of diarrhea more slowly than mice infected with the parent strain. Altogether, these results suggest that OmpA sustains the activity of the T3SS and is required for optimal virulence in EHEC. This work expands the roles of outer membrane proteins in bacterial pathogenesis.
format	article
author	Hidetada Hirakawa Kazutomo Suzue Ayako Takita Haruyoshi Tomita
author_facet	Hidetada Hirakawa Kazutomo Suzue Ayako Takita Haruyoshi Tomita
author_sort	Hidetada Hirakawa
title	Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic <i>Escherichia coli</i>
title_short	Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic <i>Escherichia coli</i>
title_full	Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic <i>Escherichia coli</i>
title_fullStr	Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic <i>Escherichia coli</i>
title_full_unstemmed	Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic <i>Escherichia coli</i>
title_sort	roles of ompa in type iii secretion system-mediated virulence of enterohemorrhagic <i>escherichia coli</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2f1c3cfb478d49339fe286e97059b89b
work_keys_str_mv	AT hidetadahirakawa rolesofompaintypeiiisecretionsystemmediatedvirulenceofenterohemorrhagiciescherichiacolii AT kazutomosuzue rolesofompaintypeiiisecretionsystemmediatedvirulenceofenterohemorrhagiciescherichiacolii AT ayakotakita rolesofompaintypeiiisecretionsystemmediatedvirulenceofenterohemorrhagiciescherichiacolii AT haruyoshitomita rolesofompaintypeiiisecretionsystemmediatedvirulenceofenterohemorrhagiciescherichiacolii
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Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic <i>Escherichia coli</i>

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