Triple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A viruses.

Compatibility among the influenza A virus (IAV) ribonucleoprotein (RNP) genes affects viral replication efficiency and can limit the emergence of novel reassortants, including those with potential pandemic risks. In this study, we determined the polymerase activities of 2,451 RNP reassortants among...

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Autores principales:	Kaitlyn Waters, Cheng Gao, Matthew Ykema, Lei Han, Lynden Voth, Yizhi Jane Tao, Xiu-Feng Wan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2f1d668579cd4c079ceb4bd338d1b620
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spelling	oai:doaj.org-article:2f1d668579cd4c079ceb4bd338d1b6202021-12-02T20:00:03ZTriple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A viruses.1553-73661553-737410.1371/journal.ppat.1009962https://doaj.org/article/2f1d668579cd4c079ceb4bd338d1b6202021-10-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009962https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Compatibility among the influenza A virus (IAV) ribonucleoprotein (RNP) genes affects viral replication efficiency and can limit the emergence of novel reassortants, including those with potential pandemic risks. In this study, we determined the polymerase activities of 2,451 RNP reassortants among three seasonal and eight enzootic IAVs by using a minigenome assay. Results showed that the 2009 H1N1 RNP are more compatible with the tested enzootic RNP than seasonal H3N2 RNP and that triple reassortment increased such compatibility. The RNP reassortants among 2009 H1N1, canine H3N8, and avian H4N6 IAVs had the highest polymerase activities. Residues in the RNA binding motifs and the contact regions among RNP proteins affected polymerase activities. Our data indicates that compatibility among seasonal and enzootic RNPs are selective, and enzoosis of multiple strains in the animal-human interface can facilitate emergence of an RNP with increased replication efficiency in mammals, including humans.Kaitlyn WatersCheng GaoMatthew YkemaLei HanLynden VothYizhi Jane TaoXiu-Feng WanPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 10, p e1009962 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Kaitlyn Waters Cheng Gao Matthew Ykema Lei Han Lynden Voth Yizhi Jane Tao Xiu-Feng Wan Triple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A viruses.
description	Compatibility among the influenza A virus (IAV) ribonucleoprotein (RNP) genes affects viral replication efficiency and can limit the emergence of novel reassortants, including those with potential pandemic risks. In this study, we determined the polymerase activities of 2,451 RNP reassortants among three seasonal and eight enzootic IAVs by using a minigenome assay. Results showed that the 2009 H1N1 RNP are more compatible with the tested enzootic RNP than seasonal H3N2 RNP and that triple reassortment increased such compatibility. The RNP reassortants among 2009 H1N1, canine H3N8, and avian H4N6 IAVs had the highest polymerase activities. Residues in the RNA binding motifs and the contact regions among RNP proteins affected polymerase activities. Our data indicates that compatibility among seasonal and enzootic RNPs are selective, and enzoosis of multiple strains in the animal-human interface can facilitate emergence of an RNP with increased replication efficiency in mammals, including humans.
format	article
author	Kaitlyn Waters Cheng Gao Matthew Ykema Lei Han Lynden Voth Yizhi Jane Tao Xiu-Feng Wan
author_facet	Kaitlyn Waters Cheng Gao Matthew Ykema Lei Han Lynden Voth Yizhi Jane Tao Xiu-Feng Wan
author_sort	Kaitlyn Waters
title	Triple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A viruses.
title_short	Triple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A viruses.
title_full	Triple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A viruses.
title_fullStr	Triple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A viruses.
title_full_unstemmed	Triple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A viruses.
title_sort	triple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza a viruses.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/2f1d668579cd4c079ceb4bd338d1b620
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Triple reassortment increases compatibility among viral ribonucleoprotein genes of contemporary avian and human influenza A viruses.

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