An ex vivo model of medical device-mediated bacterial skin translocation

Abstract The skin is a barrier and part of the immune system that protects us from harmful bacteria. Because indwelling medical devices break this barrier, they greatly increase the risk of infection by microbial pathogens. To study how these infections can be prevented through improved clinical pra...

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Autores principales:	Hao Wang, Anant Agrawal, Yi Wang, David W. Crawford, Zachary D. Siler, Marnie L. Peterson, Ricky T. Woofter, Mohamed Labib, Hainsworth Y. Shin, Andrew P. Baumann, K. Scott Phillips
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f20f4c25c4d4d219a0d60647f4587b8
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spelling	oai:doaj.org-article:2f20f4c25c4d4d219a0d60647f4587b82021-12-02T13:19:22ZAn ex vivo model of medical device-mediated bacterial skin translocation10.1038/s41598-021-84826-12045-2322https://doaj.org/article/2f20f4c25c4d4d219a0d60647f4587b82021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84826-1https://doaj.org/toc/2045-2322Abstract The skin is a barrier and part of the immune system that protects us from harmful bacteria. Because indwelling medical devices break this barrier, they greatly increase the risk of infection by microbial pathogens. To study how these infections can be prevented through improved clinical practices and medical device technology, it is important to have preclinical models that replicate the early stages of microbial contamination, ingress, and colonization leading up to infection. At present, there are no preclinical ex vivo models specifically developed to simulate conditions for indwelling medical devices. Translocation of pathogens from outside the body across broken skin to normally sterile internal compartments is a rate-limiting step in infectious pathogenesis. In this work, we report a sensitive and reproducible ex vivo porcine skin–catheter model to test how long antimicrobial interventions can delay translocation. Skin preparation was first optimized to minimize tissue damage. The presence of skin dramatically decreased bacterial migration time across the polyurethane catheter interface from > 96 h to 12 h. Using visual colony detection, fluorescence, a luminescent in vitro imaging system, and confocal microscopy, the model was used to quantify time-dependent differences in translocation for eluting and non-eluting antimicrobial catheters. The results show the importance of including tissue in preclinical biofilm models and help to explain current gaps between in vitro testing and clinical outcomes for antimicrobial devices.Hao WangAnant AgrawalYi WangDavid W. CrawfordZachary D. SilerMarnie L. PetersonRicky T. WoofterMohamed LabibHainsworth Y. ShinAndrew P. BaumannK. Scott PhillipsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hao Wang Anant Agrawal Yi Wang David W. Crawford Zachary D. Siler Marnie L. Peterson Ricky T. Woofter Mohamed Labib Hainsworth Y. Shin Andrew P. Baumann K. Scott Phillips An ex vivo model of medical device-mediated bacterial skin translocation
description	Abstract The skin is a barrier and part of the immune system that protects us from harmful bacteria. Because indwelling medical devices break this barrier, they greatly increase the risk of infection by microbial pathogens. To study how these infections can be prevented through improved clinical practices and medical device technology, it is important to have preclinical models that replicate the early stages of microbial contamination, ingress, and colonization leading up to infection. At present, there are no preclinical ex vivo models specifically developed to simulate conditions for indwelling medical devices. Translocation of pathogens from outside the body across broken skin to normally sterile internal compartments is a rate-limiting step in infectious pathogenesis. In this work, we report a sensitive and reproducible ex vivo porcine skin–catheter model to test how long antimicrobial interventions can delay translocation. Skin preparation was first optimized to minimize tissue damage. The presence of skin dramatically decreased bacterial migration time across the polyurethane catheter interface from > 96 h to 12 h. Using visual colony detection, fluorescence, a luminescent in vitro imaging system, and confocal microscopy, the model was used to quantify time-dependent differences in translocation for eluting and non-eluting antimicrobial catheters. The results show the importance of including tissue in preclinical biofilm models and help to explain current gaps between in vitro testing and clinical outcomes for antimicrobial devices.
format	article
author	Hao Wang Anant Agrawal Yi Wang David W. Crawford Zachary D. Siler Marnie L. Peterson Ricky T. Woofter Mohamed Labib Hainsworth Y. Shin Andrew P. Baumann K. Scott Phillips
author_facet	Hao Wang Anant Agrawal Yi Wang David W. Crawford Zachary D. Siler Marnie L. Peterson Ricky T. Woofter Mohamed Labib Hainsworth Y. Shin Andrew P. Baumann K. Scott Phillips
author_sort	Hao Wang
title	An ex vivo model of medical device-mediated bacterial skin translocation
title_short	An ex vivo model of medical device-mediated bacterial skin translocation
title_full	An ex vivo model of medical device-mediated bacterial skin translocation
title_fullStr	An ex vivo model of medical device-mediated bacterial skin translocation
title_full_unstemmed	An ex vivo model of medical device-mediated bacterial skin translocation
title_sort	ex vivo model of medical device-mediated bacterial skin translocation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2f20f4c25c4d4d219a0d60647f4587b8
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An ex vivo model of medical device-mediated bacterial skin translocation

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