Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia

Abstract. Nasrika E, Retnonongsih A. 2021. Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia. Biodiversitas 22: 4054-4059. Kalimantan is the center of many tropical fruits, including durian. One of the edible durians...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Evy Nasrika, Amin Retnoningsih
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2f25bbd0d05c40439a982f46410bcb8d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2f25bbd0d05c40439a982f46410bcb8d
record_format	dspace
spelling	oai:doaj.org-article:2f25bbd0d05c40439a982f46410bcb8d2021-11-22T12:18:55ZInter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia1412-033X2085-472210.13057/biodiv/d220954https://doaj.org/article/2f25bbd0d05c40439a982f46410bcb8d2021-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8737https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Nasrika E, Retnonongsih A. 2021. Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia. Biodiversitas 22: 4054-4059. Kalimantan is the center of many tropical fruits, including durian. One of the edible durians and favorited by many is Durio zibethinus, which has many superior varieties. Almost all regions in Indonesia have superior durian varieties; for example, superior durian Gunungpati from Gunungpati Sub-district, the center for producing durian in Semarang City, Central Java, Indonesia. ISSR analysis was carried out to reveal the genetic diversity of the superior durian Gunungpati. DNA isolation of 16 superior durians used the modified CTAB method. The genomic DNA was amplified using 10 ISSR primers and then electrophoresed using 2% agarose gel. Data were analyzed using NTSYS PC version 2.02. The resulting allele has a relative size of 220 bp-1800 bp, with 87.9% are polymorphic. The similarity coefficient of 16 varieties was ranged between 0.54-0.88. All the superior durian varieties examined were different accessions so that each variety has the potential to be registered as a new variety of superior Indonesian durian. Specific alleles are found in G1, G3, G7, G8, and G13, which can be an identity of these varieties.Evy NasrikaAmin RetnoningsihMBI & UNS SoloarticleBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Evy Nasrika Amin Retnoningsih Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia
description	Abstract. Nasrika E, Retnonongsih A. 2021. Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia. Biodiversitas 22: 4054-4059. Kalimantan is the center of many tropical fruits, including durian. One of the edible durians and favorited by many is Durio zibethinus, which has many superior varieties. Almost all regions in Indonesia have superior durian varieties; for example, superior durian Gunungpati from Gunungpati Sub-district, the center for producing durian in Semarang City, Central Java, Indonesia. ISSR analysis was carried out to reveal the genetic diversity of the superior durian Gunungpati. DNA isolation of 16 superior durians used the modified CTAB method. The genomic DNA was amplified using 10 ISSR primers and then electrophoresed using 2% agarose gel. Data were analyzed using NTSYS PC version 2.02. The resulting allele has a relative size of 220 bp-1800 bp, with 87.9% are polymorphic. The similarity coefficient of 16 varieties was ranged between 0.54-0.88. All the superior durian varieties examined were different accessions so that each variety has the potential to be registered as a new variety of superior Indonesian durian. Specific alleles are found in G1, G3, G7, G8, and G13, which can be an identity of these varieties.
format	article
author	Evy Nasrika Amin Retnoningsih
author_facet	Evy Nasrika Amin Retnoningsih
author_sort	Evy Nasrika
title	Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia
title_short	Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia
title_full	Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia
title_fullStr	Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia
title_full_unstemmed	Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia
title_sort	inter simple sequence repeat molecular markers to reveal the genetic diversity of superior durian of gunungpati, semarang, indonesia
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/2f25bbd0d05c40439a982f46410bcb8d
work_keys_str_mv	AT evynasrika intersimplesequencerepeatmolecularmarkerstorevealthegeneticdiversityofsuperiordurianofgunungpatisemarangindonesia AT aminretnoningsih intersimplesequencerepeatmolecularmarkerstorevealthegeneticdiversityofsuperiordurianofgunungpatisemarangindonesia
_version_	1718417601062764544

Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia

Ejemplares similares