Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers

Flower color is the decisive factor that affects the commercial value of ornamental flowers. Therefore, it is important to study the regulation of flower color formation in lily to discover the positive and negative factors that regulate this important trait. In this study, MYB transcription factors...

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Autores principales:	Xiaojuan Yin, Yibing Zhang, Li Zhang, Baohua Wang, Yidi Zhao, Muhammad Irfan, Lijing Chen, Yulong Feng
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	lily anthocyanin biosynthesis pathway MYB transcription factor activator transcriptional inhibitor promoter Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/2f26bda4cc35496498ba30a8837dd744
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spelling	oai:doaj.org-article:2f26bda4cc35496498ba30a8837dd7442021-12-02T00:34:15ZRegulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers1664-462X10.3389/fpls.2021.761668https://doaj.org/article/2f26bda4cc35496498ba30a8837dd7442021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.761668/fullhttps://doaj.org/toc/1664-462XFlower color is the decisive factor that affects the commercial value of ornamental flowers. Therefore, it is important to study the regulation of flower color formation in lily to discover the positive and negative factors that regulate this important trait. In this study, MYB transcription factors (TFs) were characterized to understand the regulatory mechanism of anthocyanin biosynthesis in lily. Two R2R3-MYB TFs, LvMYB5, and LvMYB1, were found to regulate anthocyanin biosynthesis in lily flowers. LvMYB5, which has an activation motif, belongs to the SG6 MYB protein subgroup of Arabidopsis thaliana. Transient expression of LvMYB5 indicated that LvMYB5 can promote coloration in Nicotiana benthamiana leaves, and that expression of LvMYB5 increases the expression levels of NbCHS, NbDFR, and NbANS. VIGS experiments in lily petals showed that the accumulation of anthocyanins was reduced when LvMYB5 was silenced. Luciferase assays showed that LvMYB5 can promote anthocyanin synthesis by activating the ANS gene promoter. Therefore, LvMYB5 plays an important role in flower coloration in lily. In addition, the transient expression experiment provided preliminary evidence that LvMYB1 (an R2R3-MYB TF) inhibits anthocyanin synthesis in lily flowers. The discovery of activating and inhibitory factors related to anthocyanin biosynthesis in lily provides a theoretical basis for improving flower color through genetic engineering. The results of our study provide a new direction for the further study of the mechanisms of flower color formation in lilies.Xiaojuan YinYibing ZhangLi ZhangLi ZhangBaohua WangYidi ZhaoMuhammad IrfanLijing ChenLijing ChenYulong FengYulong FengFrontiers Media S.A.articlelilyanthocyanin biosynthesis pathwayMYB transcription factoractivatortranscriptional inhibitorpromoterPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lily anthocyanin biosynthesis pathway MYB transcription factor activator transcriptional inhibitor promoter Plant culture SB1-1110
spellingShingle	lily anthocyanin biosynthesis pathway MYB transcription factor activator transcriptional inhibitor promoter Plant culture SB1-1110 Xiaojuan Yin Yibing Zhang Li Zhang Li Zhang Baohua Wang Yidi Zhao Muhammad Irfan Lijing Chen Lijing Chen Yulong Feng Yulong Feng Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers
description	Flower color is the decisive factor that affects the commercial value of ornamental flowers. Therefore, it is important to study the regulation of flower color formation in lily to discover the positive and negative factors that regulate this important trait. In this study, MYB transcription factors (TFs) were characterized to understand the regulatory mechanism of anthocyanin biosynthesis in lily. Two R2R3-MYB TFs, LvMYB5, and LvMYB1, were found to regulate anthocyanin biosynthesis in lily flowers. LvMYB5, which has an activation motif, belongs to the SG6 MYB protein subgroup of Arabidopsis thaliana. Transient expression of LvMYB5 indicated that LvMYB5 can promote coloration in Nicotiana benthamiana leaves, and that expression of LvMYB5 increases the expression levels of NbCHS, NbDFR, and NbANS. VIGS experiments in lily petals showed that the accumulation of anthocyanins was reduced when LvMYB5 was silenced. Luciferase assays showed that LvMYB5 can promote anthocyanin synthesis by activating the ANS gene promoter. Therefore, LvMYB5 plays an important role in flower coloration in lily. In addition, the transient expression experiment provided preliminary evidence that LvMYB1 (an R2R3-MYB TF) inhibits anthocyanin synthesis in lily flowers. The discovery of activating and inhibitory factors related to anthocyanin biosynthesis in lily provides a theoretical basis for improving flower color through genetic engineering. The results of our study provide a new direction for the further study of the mechanisms of flower color formation in lilies.
format	article
author	Xiaojuan Yin Yibing Zhang Li Zhang Li Zhang Baohua Wang Yidi Zhao Muhammad Irfan Lijing Chen Lijing Chen Yulong Feng Yulong Feng
author_facet	Xiaojuan Yin Yibing Zhang Li Zhang Li Zhang Baohua Wang Yidi Zhao Muhammad Irfan Lijing Chen Lijing Chen Yulong Feng Yulong Feng
author_sort	Xiaojuan Yin
title	Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers
title_short	Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers
title_full	Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers
title_fullStr	Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers
title_full_unstemmed	Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers
title_sort	regulation of myb transcription factors of anthocyanin synthesis in lily flowers
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2f26bda4cc35496498ba30a8837dd744
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_version_	1718403606430875648

Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers

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