A low-cost open-source 5-choice operant box system optimized for electrophysiology and optophysiology in mice

Abstract Operant boxes enable the application of complex behavioural paradigms to support circuit neuroscience and drug discovery research. However, commercial operant box systems are expensive and often not optimised for combining behaviour with neurophysiology. Here we introduce a fully open-sourc...

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Autores principales:	Sampath K. T. Kapanaiah, Bastiaan van der Veen, Daniel Strahnen, Thomas Akam, Dennis Kätzel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f278635aa3c4b078d5b11e1833036ac
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spelling	oai:doaj.org-article:2f278635aa3c4b078d5b11e1833036ac2021-11-21T12:24:47ZA low-cost open-source 5-choice operant box system optimized for electrophysiology and optophysiology in mice10.1038/s41598-021-01717-12045-2322https://doaj.org/article/2f278635aa3c4b078d5b11e1833036ac2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01717-1https://doaj.org/toc/2045-2322Abstract Operant boxes enable the application of complex behavioural paradigms to support circuit neuroscience and drug discovery research. However, commercial operant box systems are expensive and often not optimised for combining behaviour with neurophysiology. Here we introduce a fully open-source Python-based operant-box system in a 5-choice design (pyOS-5) that enables assessment of multiple cognitive and affective functions. It is optimized for fast turn-over between animals, and for testing of tethered mice for simultaneous physiological recordings or optogenetic manipulation. For reward delivery, we developed peristaltic and syringe pumps based on a stepper motor and 3D-printed parts. Tasks are specified using a Python-based syntax implemented on custom-designed printed circuit boards that are commercially available at low cost. We developed an open-source graphical user interface (GUI) and task definition scripts to conduct assays assessing operant learning, attention, impulsivity, working memory, or cognitive flexibility, alleviating the need for programming skills of the end user. All behavioural events are recorded with millisecond resolution, and TTL-outputs and -inputs allow straightforward integration with physiological recordings and closed-loop manipulations. This combination of features realizes a cost-effective, nose-poke-based operant box system that allows reliable circuit-neuroscience experiments investigating correlates of cognition and emotion in large cohorts of subjects.Sampath K. T. KapanaiahBastiaan van der VeenDaniel StrahnenThomas AkamDennis KätzelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sampath K. T. Kapanaiah Bastiaan van der Veen Daniel Strahnen Thomas Akam Dennis Kätzel A low-cost open-source 5-choice operant box system optimized for electrophysiology and optophysiology in mice
description	Abstract Operant boxes enable the application of complex behavioural paradigms to support circuit neuroscience and drug discovery research. However, commercial operant box systems are expensive and often not optimised for combining behaviour with neurophysiology. Here we introduce a fully open-source Python-based operant-box system in a 5-choice design (pyOS-5) that enables assessment of multiple cognitive and affective functions. It is optimized for fast turn-over between animals, and for testing of tethered mice for simultaneous physiological recordings or optogenetic manipulation. For reward delivery, we developed peristaltic and syringe pumps based on a stepper motor and 3D-printed parts. Tasks are specified using a Python-based syntax implemented on custom-designed printed circuit boards that are commercially available at low cost. We developed an open-source graphical user interface (GUI) and task definition scripts to conduct assays assessing operant learning, attention, impulsivity, working memory, or cognitive flexibility, alleviating the need for programming skills of the end user. All behavioural events are recorded with millisecond resolution, and TTL-outputs and -inputs allow straightforward integration with physiological recordings and closed-loop manipulations. This combination of features realizes a cost-effective, nose-poke-based operant box system that allows reliable circuit-neuroscience experiments investigating correlates of cognition and emotion in large cohorts of subjects.
format	article
author	Sampath K. T. Kapanaiah Bastiaan van der Veen Daniel Strahnen Thomas Akam Dennis Kätzel
author_facet	Sampath K. T. Kapanaiah Bastiaan van der Veen Daniel Strahnen Thomas Akam Dennis Kätzel
author_sort	Sampath K. T. Kapanaiah
title	A low-cost open-source 5-choice operant box system optimized for electrophysiology and optophysiology in mice
title_short	A low-cost open-source 5-choice operant box system optimized for electrophysiology and optophysiology in mice
title_full	A low-cost open-source 5-choice operant box system optimized for electrophysiology and optophysiology in mice
title_fullStr	A low-cost open-source 5-choice operant box system optimized for electrophysiology and optophysiology in mice
title_full_unstemmed	A low-cost open-source 5-choice operant box system optimized for electrophysiology and optophysiology in mice
title_sort	low-cost open-source 5-choice operant box system optimized for electrophysiology and optophysiology in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2f278635aa3c4b078d5b11e1833036ac
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A low-cost open-source 5-choice operant box system optimized for electrophysiology and optophysiology in mice

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