Origin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of <i>Allorhizobium vitis</i> Strains

Crown gall is a globally distributed and economically important disease of grapevine and other important crop plants. The causal agent of grapevine crown gall is tumorigenic <i>Allorhizobium vitis</i> (Ti) strains that harbor a tumor-inducing plasmid (pTi). The epidemic of grapevine crow...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Akira Kawaguchi, Teruo Sone, Sunao Ochi, Yosuke Matsushita, Yoshiteru Noutoshi, Mizuho Nita
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Rhizobium vitis</i> multi-locus sequence analysis grapevine crown gall vineyard epidemic Science Q
Acceso en línea:	https://doaj.org/article/2f2835a4ef6f451c9aff03172b7bfa46
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2f2835a4ef6f451c9aff03172b7bfa46
record_format	dspace
spelling	oai:doaj.org-article:2f2835a4ef6f451c9aff03172b7bfa462021-11-25T18:11:41ZOrigin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of <i>Allorhizobium vitis</i> Strains10.3390/life111112652075-1729https://doaj.org/article/2f2835a4ef6f451c9aff03172b7bfa462021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1265https://doaj.org/toc/2075-1729Crown gall is a globally distributed and economically important disease of grapevine and other important crop plants. The causal agent of grapevine crown gall is tumorigenic <i>Allorhizobium vitis</i> (Ti) strains that harbor a tumor-inducing plasmid (pTi). The epidemic of grapevine crown gall has not been widely elucidated. In this study, we investigated the genetic diversity of 89 strains of Ti and nonpathogenic <i>A. vitis</i> to clarify their molecular epidemiology. Multi-locus sequence analysis (MLSA) of the partial nucleotide sequences of <i>pyrG, recA</i>, and <i>rpoD</i> was performed for molecular typing of <i>A. vitis</i> strains isolated from grapevines with crown gall symptoms grown in 30 different vineyards, five different countries, mainly in Japan, and seven genomic groups A to F were obtained. The results of MLSA and logistic regression indicated that the population of genetic group A was significantly related to a range of prefectures and that the epidemic of group A strains originated mainly in Hokkaido in Japan through soil infection. Moreover, group E strains could have been transported by infected nursery stocks. In conclusion, this study indicates that both soil infection and transporting of infected nursery stocks are working as infection source in Hokkaido.Akira KawaguchiTeruo SoneSunao OchiYosuke MatsushitaYoshiteru NoutoshiMizuho NitaMDPI AGarticle<i>Rhizobium vitis</i>multi-locus sequence analysisgrapevine crown gallvineyardepidemicScienceQENLife, Vol 11, Iss 1265, p 1265 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Rhizobium vitis</i> multi-locus sequence analysis grapevine crown gall vineyard epidemic Science Q
spellingShingle	<i>Rhizobium vitis</i> multi-locus sequence analysis grapevine crown gall vineyard epidemic Science Q Akira Kawaguchi Teruo Sone Sunao Ochi Yosuke Matsushita Yoshiteru Noutoshi Mizuho Nita Origin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of <i>Allorhizobium vitis</i> Strains
description	Crown gall is a globally distributed and economically important disease of grapevine and other important crop plants. The causal agent of grapevine crown gall is tumorigenic <i>Allorhizobium vitis</i> (Ti) strains that harbor a tumor-inducing plasmid (pTi). The epidemic of grapevine crown gall has not been widely elucidated. In this study, we investigated the genetic diversity of 89 strains of Ti and nonpathogenic <i>A. vitis</i> to clarify their molecular epidemiology. Multi-locus sequence analysis (MLSA) of the partial nucleotide sequences of <i>pyrG, recA</i>, and <i>rpoD</i> was performed for molecular typing of <i>A. vitis</i> strains isolated from grapevines with crown gall symptoms grown in 30 different vineyards, five different countries, mainly in Japan, and seven genomic groups A to F were obtained. The results of MLSA and logistic regression indicated that the population of genetic group A was significantly related to a range of prefectures and that the epidemic of group A strains originated mainly in Hokkaido in Japan through soil infection. Moreover, group E strains could have been transported by infected nursery stocks. In conclusion, this study indicates that both soil infection and transporting of infected nursery stocks are working as infection source in Hokkaido.
format	article
author	Akira Kawaguchi Teruo Sone Sunao Ochi Yosuke Matsushita Yoshiteru Noutoshi Mizuho Nita
author_facet	Akira Kawaguchi Teruo Sone Sunao Ochi Yosuke Matsushita Yoshiteru Noutoshi Mizuho Nita
author_sort	Akira Kawaguchi
title	Origin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of <i>Allorhizobium vitis</i> Strains
title_short	Origin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of <i>Allorhizobium vitis</i> Strains
title_full	Origin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of <i>Allorhizobium vitis</i> Strains
title_fullStr	Origin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of <i>Allorhizobium vitis</i> Strains
title_full_unstemmed	Origin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of <i>Allorhizobium vitis</i> Strains
title_sort	origin of pathogens of grapevine crown gall disease in hokkaido in japan as characterized by molecular epidemiology of <i>allorhizobium vitis</i> strains
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2f2835a4ef6f451c9aff03172b7bfa46
work_keys_str_mv	AT akirakawaguchi originofpathogensofgrapevinecrowngalldiseaseinhokkaidoinjapanascharacterizedbymolecularepidemiologyofiallorhizobiumvitisistrains AT teruosone originofpathogensofgrapevinecrowngalldiseaseinhokkaidoinjapanascharacterizedbymolecularepidemiologyofiallorhizobiumvitisistrains AT sunaoochi originofpathogensofgrapevinecrowngalldiseaseinhokkaidoinjapanascharacterizedbymolecularepidemiologyofiallorhizobiumvitisistrains AT yosukematsushita originofpathogensofgrapevinecrowngalldiseaseinhokkaidoinjapanascharacterizedbymolecularepidemiologyofiallorhizobiumvitisistrains AT yoshiterunoutoshi originofpathogensofgrapevinecrowngalldiseaseinhokkaidoinjapanascharacterizedbymolecularepidemiologyofiallorhizobiumvitisistrains AT mizuhonita originofpathogensofgrapevinecrowngalldiseaseinhokkaidoinjapanascharacterizedbymolecularepidemiologyofiallorhizobiumvitisistrains
_version_	1718411489335836672

Origin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of <i>Allorhizobium vitis</i> Strains

Ejemplares similares