Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina

The aim of this study is to provide preliminary data about the occurrence of Neospora caninum- and Toxoplasma gondii-related abortions and perinatal deaths in sheep from Argentina. Thirty ovine aborted foetuses and 33 perinatal deaths were submitted to the Veterinary Diagnostic Service at INTA EEA B...

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Autores principales:	Paola Della Rosa, María A. Fiorentino, Eleonora L. Morrell, María V. Scioli, Fernando A. Paolicchi, Dadín P. Moore, Germán J. Cantón, Yanina P. Hecker
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Neospora caninum Toxoplasma gondii Abortion Perinatal death Sheep Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/2f29433ed5834a1090a4bf1613b366e8
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spelling	oai:doaj.org-article:2f29433ed5834a1090a4bf1613b366e82021-11-18T04:53:48ZNeospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina2667-114X10.1016/j.crpvbd.2021.100057https://doaj.org/article/2f29433ed5834a1090a4bf1613b366e82021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667114X21000510https://doaj.org/toc/2667-114XThe aim of this study is to provide preliminary data about the occurrence of Neospora caninum- and Toxoplasma gondii-related abortions and perinatal deaths in sheep from Argentina. Thirty ovine aborted foetuses and 33 perinatal deaths were submitted to the Veterinary Diagnostic Service at INTA EEA Balcarce (Argentina) during 2017–2019. A complete necropsy was performed on all specimens submitted, and foetal and placental tissues were examined. Foetal cavity fluids were collected for assessment of antibodies to N. caninum and T. gondii by indirect fluorescent antibody technique (IFAT). Placental and foetal tissue samples were collected for DNA extraction and histopathological analysis. The differential diagnosis with other causes of abortion was carried out. Of the sampled specimens, 20.63% (13/63) displayed evidence for N. caninum infection by IFAT and PCR, and in 61.5% (8/13) of the positive specimens the parasite was confirmed as the cause of abortion/perinatal death based on the presence of compatible histological lesions and/or positive immunohistochemistry test, positive PCR and/or positive IFAT, and no other infectious agents diagnosed. In contrast, T. gondii infection was confirmed in 9.52% (6/63) of the analysed specimens, but only in 2 lambs T. gondii was determined as the death cause. Neospora caninum and T. gondii co-infections were confirmed in 4 analysed specimens (2 aborted foetuses and 2 perinatal deaths). These results demonstrated that N. caninum is efficiently transmitted and a frequent cause of ovine reproductive failure in the commercial analysed flocks compared with T. gondii. Despite T. gondii congenital infection was detected in some specimens (6/63), it was confirmed as the cause of death in only two of them. Thus, and considering the limited availability of confirmed samples, we could not determine whether toxoplasmosis is a major problem in Argentinian sheep flocks or not. More studies on a greater number of specimens from different ovine production systems under different management conditions are necessary to assess the real impact of neosporosis and toxoplasmosis in Argentina.Paola Della RosaMaría A. FiorentinoEleonora L. MorrellMaría V. ScioliFernando A. PaolicchiDadín P. MooreGermán J. CantónYanina P. HeckerElsevierarticleNeospora caninumToxoplasma gondiiAbortionPerinatal deathSheepInfectious and parasitic diseasesRC109-216ENCurrent Research in Parasitology and Vector-Borne Diseases, Vol 1, Iss , Pp 100057- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neospora caninum Toxoplasma gondii Abortion Perinatal death Sheep Infectious and parasitic diseases RC109-216
spellingShingle	Neospora caninum Toxoplasma gondii Abortion Perinatal death Sheep Infectious and parasitic diseases RC109-216 Paola Della Rosa María A. Fiorentino Eleonora L. Morrell María V. Scioli Fernando A. Paolicchi Dadín P. Moore Germán J. Cantón Yanina P. Hecker Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina
description	The aim of this study is to provide preliminary data about the occurrence of Neospora caninum- and Toxoplasma gondii-related abortions and perinatal deaths in sheep from Argentina. Thirty ovine aborted foetuses and 33 perinatal deaths were submitted to the Veterinary Diagnostic Service at INTA EEA Balcarce (Argentina) during 2017–2019. A complete necropsy was performed on all specimens submitted, and foetal and placental tissues were examined. Foetal cavity fluids were collected for assessment of antibodies to N. caninum and T. gondii by indirect fluorescent antibody technique (IFAT). Placental and foetal tissue samples were collected for DNA extraction and histopathological analysis. The differential diagnosis with other causes of abortion was carried out. Of the sampled specimens, 20.63% (13/63) displayed evidence for N. caninum infection by IFAT and PCR, and in 61.5% (8/13) of the positive specimens the parasite was confirmed as the cause of abortion/perinatal death based on the presence of compatible histological lesions and/or positive immunohistochemistry test, positive PCR and/or positive IFAT, and no other infectious agents diagnosed. In contrast, T. gondii infection was confirmed in 9.52% (6/63) of the analysed specimens, but only in 2 lambs T. gondii was determined as the death cause. Neospora caninum and T. gondii co-infections were confirmed in 4 analysed specimens (2 aborted foetuses and 2 perinatal deaths). These results demonstrated that N. caninum is efficiently transmitted and a frequent cause of ovine reproductive failure in the commercial analysed flocks compared with T. gondii. Despite T. gondii congenital infection was detected in some specimens (6/63), it was confirmed as the cause of death in only two of them. Thus, and considering the limited availability of confirmed samples, we could not determine whether toxoplasmosis is a major problem in Argentinian sheep flocks or not. More studies on a greater number of specimens from different ovine production systems under different management conditions are necessary to assess the real impact of neosporosis and toxoplasmosis in Argentina.
format	article
author	Paola Della Rosa María A. Fiorentino Eleonora L. Morrell María V. Scioli Fernando A. Paolicchi Dadín P. Moore Germán J. Cantón Yanina P. Hecker
author_facet	Paola Della Rosa María A. Fiorentino Eleonora L. Morrell María V. Scioli Fernando A. Paolicchi Dadín P. Moore Germán J. Cantón Yanina P. Hecker
author_sort	Paola Della Rosa
title	Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina
title_short	Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina
title_full	Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina
title_fullStr	Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina
title_full_unstemmed	Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina
title_sort	neospora caninum and toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from argentina
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2f29433ed5834a1090a4bf1613b366e8
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Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina

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