Transcriptome analysis reveals antioxidant defense mechanisms in the red swamp crayfish Procambarus clarkia after exposure to chromium

Chromium (Cr) as a chromate anion has a strong redox capacity that seriously threatens the ecological environment and human health. Cr can contaminate water and impart toxicity to aquatic species. Procambarus clarkii is an important food source that once represented a large proportion of the aquacul...

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Autores principales:	Qiu-Ning Liu, Ying-Yu Tang, Jing-Ru Zhao, Yue-Tian Li, Rui-Ping Yang, Dai-Zhen Zhang, Yong-Xu Cheng, Bo-Ping Tang, Feng Ding
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Procambarus clarkii Cr Differentially expressed genes Hemocytes Antioxidant defense mechanisms Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/2f29bff1be814235abde00eadbadcd72
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spelling	oai:doaj.org-article:2f29bff1be814235abde00eadbadcd722021-11-06T04:16:52ZTranscriptome analysis reveals antioxidant defense mechanisms in the red swamp crayfish Procambarus clarkia after exposure to chromium0147-651310.1016/j.ecoenv.2021.112911https://doaj.org/article/2f29bff1be814235abde00eadbadcd722021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S014765132101023Xhttps://doaj.org/toc/0147-6513Chromium (Cr) as a chromate anion has a strong redox capacity that seriously threatens the ecological environment and human health. Cr can contaminate water and impart toxicity to aquatic species. Procambarus clarkii is an important food source that once represented a large proportion of the aquaculture industry due to its rapid reproduction and high economic value. However, there have been reports on the death of P. clarkii due to heavy metal pollution. The underlying mechanism regarding heavy metal toxicity was studied in this paper. The transcriptome data of hemocytes extracted from P. clarkii injected with Cr were analyzed by high-throughput sequencing and compared to the control group. In total, 48,128,748 clean reads were obtained in the treatment group and 56,480,556 clean reads were obtained in the control group. The reads were assembled using Trinity and the identified unigenes were then annotated. Then, 421 differentially-expressed genes (DEGs) were found, 170 of which were upregulated and 251 downregulated. Many of these genes were found to be related to glutathione metabolism and transportation. The glutathione metabolic pathway of P. clarkii was thus activated by Cr exposure to detoxify and maintain body function. Validation of DEGs with quantitative real-time PCR confirms the changes in gene expression. Thus, this study provides data supporting a glutathione-focused response of P. clarkii to exposure to heavy metals.Qiu-Ning LiuYing-Yu TangJing-Ru ZhaoYue-Tian LiRui-Ping YangDai-Zhen ZhangYong-Xu ChengBo-Ping TangFeng DingElsevierarticleProcambarus clarkiiCrDifferentially expressed genesHemocytesAntioxidant defense mechanismsEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112911- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Procambarus clarkii Cr Differentially expressed genes Hemocytes Antioxidant defense mechanisms Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Procambarus clarkii Cr Differentially expressed genes Hemocytes Antioxidant defense mechanisms Environmental pollution TD172-193.5 Environmental sciences GE1-350 Qiu-Ning Liu Ying-Yu Tang Jing-Ru Zhao Yue-Tian Li Rui-Ping Yang Dai-Zhen Zhang Yong-Xu Cheng Bo-Ping Tang Feng Ding Transcriptome analysis reveals antioxidant defense mechanisms in the red swamp crayfish Procambarus clarkia after exposure to chromium
description	Chromium (Cr) as a chromate anion has a strong redox capacity that seriously threatens the ecological environment and human health. Cr can contaminate water and impart toxicity to aquatic species. Procambarus clarkii is an important food source that once represented a large proportion of the aquaculture industry due to its rapid reproduction and high economic value. However, there have been reports on the death of P. clarkii due to heavy metal pollution. The underlying mechanism regarding heavy metal toxicity was studied in this paper. The transcriptome data of hemocytes extracted from P. clarkii injected with Cr were analyzed by high-throughput sequencing and compared to the control group. In total, 48,128,748 clean reads were obtained in the treatment group and 56,480,556 clean reads were obtained in the control group. The reads were assembled using Trinity and the identified unigenes were then annotated. Then, 421 differentially-expressed genes (DEGs) were found, 170 of which were upregulated and 251 downregulated. Many of these genes were found to be related to glutathione metabolism and transportation. The glutathione metabolic pathway of P. clarkii was thus activated by Cr exposure to detoxify and maintain body function. Validation of DEGs with quantitative real-time PCR confirms the changes in gene expression. Thus, this study provides data supporting a glutathione-focused response of P. clarkii to exposure to heavy metals.
format	article
author	Qiu-Ning Liu Ying-Yu Tang Jing-Ru Zhao Yue-Tian Li Rui-Ping Yang Dai-Zhen Zhang Yong-Xu Cheng Bo-Ping Tang Feng Ding
author_facet	Qiu-Ning Liu Ying-Yu Tang Jing-Ru Zhao Yue-Tian Li Rui-Ping Yang Dai-Zhen Zhang Yong-Xu Cheng Bo-Ping Tang Feng Ding
author_sort	Qiu-Ning Liu
title	Transcriptome analysis reveals antioxidant defense mechanisms in the red swamp crayfish Procambarus clarkia after exposure to chromium
title_short	Transcriptome analysis reveals antioxidant defense mechanisms in the red swamp crayfish Procambarus clarkia after exposure to chromium
title_full	Transcriptome analysis reveals antioxidant defense mechanisms in the red swamp crayfish Procambarus clarkia after exposure to chromium
title_fullStr	Transcriptome analysis reveals antioxidant defense mechanisms in the red swamp crayfish Procambarus clarkia after exposure to chromium
title_full_unstemmed	Transcriptome analysis reveals antioxidant defense mechanisms in the red swamp crayfish Procambarus clarkia after exposure to chromium
title_sort	transcriptome analysis reveals antioxidant defense mechanisms in the red swamp crayfish procambarus clarkia after exposure to chromium
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2f29bff1be814235abde00eadbadcd72
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Transcriptome analysis reveals antioxidant defense mechanisms in the red swamp crayfish Procambarus clarkia after exposure to chromium

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