Detection of Neisseria meningitidis in saliva and oropharyngeal samples from college students

Abstract Carriage of Neisseria meningitidis is an accepted endpoint in monitoring meningococcal vaccines effects. We have assessed N. meningitidis and vaccine-type genogroup carriage prevalence in college students at the time of MenACWY vaccine introduction in the Netherlands, and evaluated the feas...

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Autores principales:	Willem R. Miellet, Rob Mariman, Gerlinde Pluister, Lieke J. de Jong, Ivo Grift, Stijn Wijkstra, Elske M. van Logchem, Janieke van Veldhuizen, Marie-Monique Immink, Alienke J. Wijmenga-Monsuur, Nynke Y. Rots, Elisabeth A. M. Sanders, Thijs Bosch, Krzysztof Trzciński
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f2d0c4a30eb4335a637caec42afd1f7
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spelling	oai:doaj.org-article:2f2d0c4a30eb4335a637caec42afd1f72021-12-05T12:12:35ZDetection of Neisseria meningitidis in saliva and oropharyngeal samples from college students10.1038/s41598-021-02555-x2045-2322https://doaj.org/article/2f2d0c4a30eb4335a637caec42afd1f72021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02555-xhttps://doaj.org/toc/2045-2322Abstract Carriage of Neisseria meningitidis is an accepted endpoint in monitoring meningococcal vaccines effects. We have assessed N. meningitidis and vaccine-type genogroup carriage prevalence in college students at the time of MenACWY vaccine introduction in the Netherlands, and evaluated the feasibility of saliva sampling for the surveillance of carriage. For this, paired saliva and oropharyngeal samples collected from 299 students were cultured for meningococcus. The DNA extracted from all bacterial growth was subjected to qPCRs quantifying meningococcal and genogroup-specific genes presence. Samples negative by culture yet positive for qPCR were cultured again for meningococcus. Altogether 74 (25%) of students were identified as meningococcal carrier by any method. Sixty-one students (20%) were identified as carriers with qPCR. The difference between number of qPCR-positive oropharyngeal (n = 59) and saliva (n = 52) samples was not significant (McNemar’s test, p = 0.07). Meningococci were cultured from 72 students (24%), with a significantly higher (p < 0.001) number of oropharyngeal (n = 70) compared with saliva (n = 54) samples. The prevalence of genogroups A, B, C, W, and Y was none, 9%, 1%, 1% and 6%, respectively, and 8% of students carried MenACWY vaccine-type genogroup meningococci. Saliva is easy to collect and when combined with qPCR detection can be considered for meningococcal carriage studies.Willem R. MielletRob MarimanGerlinde PluisterLieke J. de JongIvo GriftStijn WijkstraElske M. van LogchemJanieke van VeldhuizenMarie-Monique ImminkAlienke J. Wijmenga-MonsuurNynke Y. RotsElisabeth A. M. SandersThijs BoschKrzysztof TrzcińskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Willem R. Miellet Rob Mariman Gerlinde Pluister Lieke J. de Jong Ivo Grift Stijn Wijkstra Elske M. van Logchem Janieke van Veldhuizen Marie-Monique Immink Alienke J. Wijmenga-Monsuur Nynke Y. Rots Elisabeth A. M. Sanders Thijs Bosch Krzysztof Trzciński Detection of Neisseria meningitidis in saliva and oropharyngeal samples from college students
description	Abstract Carriage of Neisseria meningitidis is an accepted endpoint in monitoring meningococcal vaccines effects. We have assessed N. meningitidis and vaccine-type genogroup carriage prevalence in college students at the time of MenACWY vaccine introduction in the Netherlands, and evaluated the feasibility of saliva sampling for the surveillance of carriage. For this, paired saliva and oropharyngeal samples collected from 299 students were cultured for meningococcus. The DNA extracted from all bacterial growth was subjected to qPCRs quantifying meningococcal and genogroup-specific genes presence. Samples negative by culture yet positive for qPCR were cultured again for meningococcus. Altogether 74 (25%) of students were identified as meningococcal carrier by any method. Sixty-one students (20%) were identified as carriers with qPCR. The difference between number of qPCR-positive oropharyngeal (n = 59) and saliva (n = 52) samples was not significant (McNemar’s test, p = 0.07). Meningococci were cultured from 72 students (24%), with a significantly higher (p < 0.001) number of oropharyngeal (n = 70) compared with saliva (n = 54) samples. The prevalence of genogroups A, B, C, W, and Y was none, 9%, 1%, 1% and 6%, respectively, and 8% of students carried MenACWY vaccine-type genogroup meningococci. Saliva is easy to collect and when combined with qPCR detection can be considered for meningococcal carriage studies.
format	article
author	Willem R. Miellet Rob Mariman Gerlinde Pluister Lieke J. de Jong Ivo Grift Stijn Wijkstra Elske M. van Logchem Janieke van Veldhuizen Marie-Monique Immink Alienke J. Wijmenga-Monsuur Nynke Y. Rots Elisabeth A. M. Sanders Thijs Bosch Krzysztof Trzciński
author_facet	Willem R. Miellet Rob Mariman Gerlinde Pluister Lieke J. de Jong Ivo Grift Stijn Wijkstra Elske M. van Logchem Janieke van Veldhuizen Marie-Monique Immink Alienke J. Wijmenga-Monsuur Nynke Y. Rots Elisabeth A. M. Sanders Thijs Bosch Krzysztof Trzciński
author_sort	Willem R. Miellet
title	Detection of Neisseria meningitidis in saliva and oropharyngeal samples from college students
title_short	Detection of Neisseria meningitidis in saliva and oropharyngeal samples from college students
title_full	Detection of Neisseria meningitidis in saliva and oropharyngeal samples from college students
title_fullStr	Detection of Neisseria meningitidis in saliva and oropharyngeal samples from college students
title_full_unstemmed	Detection of Neisseria meningitidis in saliva and oropharyngeal samples from college students
title_sort	detection of neisseria meningitidis in saliva and oropharyngeal samples from college students
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2f2d0c4a30eb4335a637caec42afd1f7
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Detection of Neisseria meningitidis in saliva and oropharyngeal samples from college students

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