Neural Derivates of Canine Induced Pluripotent Stem Cells-Like Cells From a Mild Cognitive Impairment Dog

Domestic dogs are superior models for translational medicine due to greater anatomical and physiological similarities with humans than rodents, including hereditary diseases with human equivalents. Particularly with respect to neurodegenerative medicine, dogs can serve as a natural, more relevant mo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Abinaya Chandrasekaran, Barbara Blicher Thomsen, Jørgen Steen Agerholm, Laís Vicari de Figueiredo Pessôa, Naira Caroline Godoy Pieri, Vahideh Sabaghidarmiyan, Katarina Langley, Miriam Kolko, André Furugen Cesar de Andrade, Fabiana Fernandes Bressan, Poul Hyttel, Mette Berendt, Kristine Freude
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	dog iPSCs cognitive dysfunction neurons ciPSCLC Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/2f2d3f173c754a9d8d7bae6797ecc0fc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2f2d3f173c754a9d8d7bae6797ecc0fc
record_format	dspace
spelling	oai:doaj.org-article:2f2d3f173c754a9d8d7bae6797ecc0fc2021-11-04T07:01:44ZNeural Derivates of Canine Induced Pluripotent Stem Cells-Like Cells From a Mild Cognitive Impairment Dog2297-176910.3389/fvets.2021.725386https://doaj.org/article/2f2d3f173c754a9d8d7bae6797ecc0fc2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.725386/fullhttps://doaj.org/toc/2297-1769Domestic dogs are superior models for translational medicine due to greater anatomical and physiological similarities with humans than rodents, including hereditary diseases with human equivalents. Particularly with respect to neurodegenerative medicine, dogs can serve as a natural, more relevant model of human disease compared to transgenic rodents. Herein we report attempts to develop a canine-derived in vitro model for neurodegenerative diseases through the generation of induced pluripotent stem cells from a 14-year, 9-month-old female West Highland white terrier with mild cognitive impairment (MCI). Canine induced pluripotent stem cells-like cells (ciPSCLC) were generated using human OSKM and characterized by positive expression of pluripotency markers. Due to inefficient viral vector silencing we refer to them as ciPSCLCs. Subsequently, the ciPSCLC were subjected to neural induction according to two protocols both yielding canine neural progenitor cells (cNPCs), which expressed typical NPC markers. The cNPCs were cultured in neuron differentiation media for 3 weeks, resulting in the derivation of morphologically impaired neurons as compared to iPSC-derived human counterparts generated in parallel. The apparent differences encountered in this study regarding the neural differentiation potential of ciPSCLC reveals challenges and new perspectives to consider before using the canine model in translational neurological studies.Abinaya ChandrasekaranBarbara Blicher ThomsenJørgen Steen AgerholmLaís Vicari de Figueiredo PessôaNaira Caroline Godoy PieriVahideh SabaghidarmiyanKatarina LangleyMiriam KolkoAndré Furugen Cesar de AndradeFabiana Fernandes BressanPoul HyttelMette BerendtKristine FreudeFrontiers Media S.A.articledogiPSCscognitive dysfunctionneuronsciPSCLCVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dog iPSCs cognitive dysfunction neurons ciPSCLC Veterinary medicine SF600-1100
spellingShingle	dog iPSCs cognitive dysfunction neurons ciPSCLC Veterinary medicine SF600-1100 Abinaya Chandrasekaran Barbara Blicher Thomsen Jørgen Steen Agerholm Laís Vicari de Figueiredo Pessôa Naira Caroline Godoy Pieri Vahideh Sabaghidarmiyan Katarina Langley Miriam Kolko André Furugen Cesar de Andrade Fabiana Fernandes Bressan Poul Hyttel Mette Berendt Kristine Freude Neural Derivates of Canine Induced Pluripotent Stem Cells-Like Cells From a Mild Cognitive Impairment Dog
description	Domestic dogs are superior models for translational medicine due to greater anatomical and physiological similarities with humans than rodents, including hereditary diseases with human equivalents. Particularly with respect to neurodegenerative medicine, dogs can serve as a natural, more relevant model of human disease compared to transgenic rodents. Herein we report attempts to develop a canine-derived in vitro model for neurodegenerative diseases through the generation of induced pluripotent stem cells from a 14-year, 9-month-old female West Highland white terrier with mild cognitive impairment (MCI). Canine induced pluripotent stem cells-like cells (ciPSCLC) were generated using human OSKM and characterized by positive expression of pluripotency markers. Due to inefficient viral vector silencing we refer to them as ciPSCLCs. Subsequently, the ciPSCLC were subjected to neural induction according to two protocols both yielding canine neural progenitor cells (cNPCs), which expressed typical NPC markers. The cNPCs were cultured in neuron differentiation media for 3 weeks, resulting in the derivation of morphologically impaired neurons as compared to iPSC-derived human counterparts generated in parallel. The apparent differences encountered in this study regarding the neural differentiation potential of ciPSCLC reveals challenges and new perspectives to consider before using the canine model in translational neurological studies.
format	article
author	Abinaya Chandrasekaran Barbara Blicher Thomsen Jørgen Steen Agerholm Laís Vicari de Figueiredo Pessôa Naira Caroline Godoy Pieri Vahideh Sabaghidarmiyan Katarina Langley Miriam Kolko André Furugen Cesar de Andrade Fabiana Fernandes Bressan Poul Hyttel Mette Berendt Kristine Freude
author_facet	Abinaya Chandrasekaran Barbara Blicher Thomsen Jørgen Steen Agerholm Laís Vicari de Figueiredo Pessôa Naira Caroline Godoy Pieri Vahideh Sabaghidarmiyan Katarina Langley Miriam Kolko André Furugen Cesar de Andrade Fabiana Fernandes Bressan Poul Hyttel Mette Berendt Kristine Freude
author_sort	Abinaya Chandrasekaran
title	Neural Derivates of Canine Induced Pluripotent Stem Cells-Like Cells From a Mild Cognitive Impairment Dog
title_short	Neural Derivates of Canine Induced Pluripotent Stem Cells-Like Cells From a Mild Cognitive Impairment Dog
title_full	Neural Derivates of Canine Induced Pluripotent Stem Cells-Like Cells From a Mild Cognitive Impairment Dog
title_fullStr	Neural Derivates of Canine Induced Pluripotent Stem Cells-Like Cells From a Mild Cognitive Impairment Dog
title_full_unstemmed	Neural Derivates of Canine Induced Pluripotent Stem Cells-Like Cells From a Mild Cognitive Impairment Dog
title_sort	neural derivates of canine induced pluripotent stem cells-like cells from a mild cognitive impairment dog
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2f2d3f173c754a9d8d7bae6797ecc0fc
work_keys_str_mv	AT abinayachandrasekaran neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT barbarablicherthomsen neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT jørgensteenagerholm neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT laisvicaridefigueiredopessoa neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT nairacarolinegodoypieri neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT vahidehsabaghidarmiyan neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT katarinalangley neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT miriamkolko neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT andrefurugencesardeandrade neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT fabianafernandesbressan neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT poulhyttel neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT metteberendt neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog AT kristinefreude neuralderivatesofcanineinducedpluripotentstemcellslikecellsfromamildcognitiveimpairmentdog
_version_	1718445010675826688

Neural Derivates of Canine Induced Pluripotent Stem Cells-Like Cells From a Mild Cognitive Impairment Dog

Ejemplares similares