The role of coral-associated bacterial communities in Australian Subtropical White Syndrome of Turbinaria mesenterina.

Australian Subtropical White Syndrome (ASWS) is an infectious, temperature dependent disease of the subtropical coral Turbinaria mesenterina involving a hitherto unknown transmissible causative agent. This report describes significant changes in the coral associated bacterial community as the diseas...

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Autores principales:	Scott Godwin, Elizabeth Bent, James Borneman, Lily Pereg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f2f681bd0b24d2c89297453a110af5f
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spelling	oai:doaj.org-article:2f2f681bd0b24d2c89297453a110af5f2021-11-18T07:06:25ZThe role of coral-associated bacterial communities in Australian Subtropical White Syndrome of Turbinaria mesenterina.1932-620310.1371/journal.pone.0044243https://doaj.org/article/2f2f681bd0b24d2c89297453a110af5f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22970188/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Australian Subtropical White Syndrome (ASWS) is an infectious, temperature dependent disease of the subtropical coral Turbinaria mesenterina involving a hitherto unknown transmissible causative agent. This report describes significant changes in the coral associated bacterial community as the disease progresses from the apparently healthy tissue of ASWS affected coral colonies, to areas of the colony affected by ASWS lesions, to the dead coral skeleton exposed by ASWS. In an effort to better understand the potential roles of bacteria in the formation of disease lesions, the effect of antibacterials on the rate of lesion progression was tested, and both culture based and culture independent techniques were used to investigate the bacterial communities associated with colonies of T. mesenterina. Culture-independent analysis was performed using the Oligonucleotide Fingerprinting of Ribosomal Genes (OFRG) technique, which allowed a library of 8094 cloned bacterial 16S ribosomal genes to be analysed. Interestingly, the bacterial communities associated with both healthy and disease affected corals were very diverse and ASWS associated communities were not characterized by a single dominant organism. Treatment with antibacterials had a significant effect on the rate of progress of disease lesions (p = 0.006), suggesting that bacteria may play direct roles as the causative agents of ASWS. A number of potential aetiological agents of ASWS were identified in both the culture-based and culture-independent studies. In the culture-independent study an Alphaproteobacterium closely related to Roseovarius crassostreae, the apparent aetiological agent of juvenile oyster disease, was found to be significantly associated with disease lesions. In the culture-based study Vibrio harveyi was consistently associated with ASWS affected coral colonies and was not isolated from any healthy colonies. The differing results of the culture based and culture-independent studies highlight the importance of using both approaches in the investigation of microbial communities.Scott GodwinElizabeth BentJames BornemanLily PeregPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e44243 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Scott Godwin Elizabeth Bent James Borneman Lily Pereg The role of coral-associated bacterial communities in Australian Subtropical White Syndrome of Turbinaria mesenterina.
description	Australian Subtropical White Syndrome (ASWS) is an infectious, temperature dependent disease of the subtropical coral Turbinaria mesenterina involving a hitherto unknown transmissible causative agent. This report describes significant changes in the coral associated bacterial community as the disease progresses from the apparently healthy tissue of ASWS affected coral colonies, to areas of the colony affected by ASWS lesions, to the dead coral skeleton exposed by ASWS. In an effort to better understand the potential roles of bacteria in the formation of disease lesions, the effect of antibacterials on the rate of lesion progression was tested, and both culture based and culture independent techniques were used to investigate the bacterial communities associated with colonies of T. mesenterina. Culture-independent analysis was performed using the Oligonucleotide Fingerprinting of Ribosomal Genes (OFRG) technique, which allowed a library of 8094 cloned bacterial 16S ribosomal genes to be analysed. Interestingly, the bacterial communities associated with both healthy and disease affected corals were very diverse and ASWS associated communities were not characterized by a single dominant organism. Treatment with antibacterials had a significant effect on the rate of progress of disease lesions (p = 0.006), suggesting that bacteria may play direct roles as the causative agents of ASWS. A number of potential aetiological agents of ASWS were identified in both the culture-based and culture-independent studies. In the culture-independent study an Alphaproteobacterium closely related to Roseovarius crassostreae, the apparent aetiological agent of juvenile oyster disease, was found to be significantly associated with disease lesions. In the culture-based study Vibrio harveyi was consistently associated with ASWS affected coral colonies and was not isolated from any healthy colonies. The differing results of the culture based and culture-independent studies highlight the importance of using both approaches in the investigation of microbial communities.
format	article
author	Scott Godwin Elizabeth Bent James Borneman Lily Pereg
author_facet	Scott Godwin Elizabeth Bent James Borneman Lily Pereg
author_sort	Scott Godwin
title	The role of coral-associated bacterial communities in Australian Subtropical White Syndrome of Turbinaria mesenterina.
title_short	The role of coral-associated bacterial communities in Australian Subtropical White Syndrome of Turbinaria mesenterina.
title_full	The role of coral-associated bacterial communities in Australian Subtropical White Syndrome of Turbinaria mesenterina.
title_fullStr	The role of coral-associated bacterial communities in Australian Subtropical White Syndrome of Turbinaria mesenterina.
title_full_unstemmed	The role of coral-associated bacterial communities in Australian Subtropical White Syndrome of Turbinaria mesenterina.
title_sort	role of coral-associated bacterial communities in australian subtropical white syndrome of turbinaria mesenterina.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2f2f681bd0b24d2c89297453a110af5f
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The role of coral-associated bacterial communities in Australian Subtropical White Syndrome of Turbinaria mesenterina.

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