The Lotus japonicus ROP3 Is Involved in the Establishment of the Nitrogen-Fixing Symbiosis but Not of the Arbuscular Mycorrhizal Symbiosis

Legumes form root mutualistic symbioses with some soil microbes promoting their growth, rhizobia, and arbuscular mycorrhizal fungi (AMF). A conserved set of plant proteins rules the transduction of symbiotic signals from rhizobia and AMF in a so-called common symbiotic signaling pathway (CSSP). Desp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ivette García-Soto, Raphael Boussageon, Yareni Marlene Cruz-Farfán, Jesus Daniel Castro-Chilpa, Liz Xochiquetzal Hernández-Cerezo, Victor Bustos-Zagal, Alfonso Leija-Salas, Georgina Hernández, Martha Torres, Damien Formey, Pierre-Emmanuel Courty, Daniel Wipf, Mario Serrano, Alexandre Tromas
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	symbiotic nitrogen fixation ROP rho-GTPase Lotus japonicus arbuscular mycorrhizal symbiosis Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/2f2fa542800d452f9083c8fb1d999e0a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2f2fa542800d452f9083c8fb1d999e0a
record_format	dspace
spelling	oai:doaj.org-article:2f2fa542800d452f9083c8fb1d999e0a2021-11-12T04:43:39ZThe Lotus japonicus ROP3 Is Involved in the Establishment of the Nitrogen-Fixing Symbiosis but Not of the Arbuscular Mycorrhizal Symbiosis1664-462X10.3389/fpls.2021.696450https://doaj.org/article/2f2fa542800d452f9083c8fb1d999e0a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.696450/fullhttps://doaj.org/toc/1664-462XLegumes form root mutualistic symbioses with some soil microbes promoting their growth, rhizobia, and arbuscular mycorrhizal fungi (AMF). A conserved set of plant proteins rules the transduction of symbiotic signals from rhizobia and AMF in a so-called common symbiotic signaling pathway (CSSP). Despite considerable efforts and advances over the past 20 years, there are still key elements to be discovered about the establishment of these root symbioses. Rhizobia and AMF root colonization are possible after a deep cell reorganization. In the interaction between the model legume Lotus japonicus and Mesorhizobium loti, this reorganization has been shown to be dependent on a SCAR/Wave-like signaling module, including Rho-GTPase (ROP in plants). Here, we studied the potential role of ROP3 in the nitrogen-fixing symbiosis (NFS) as well as in the arbuscular mycorrhizal symbiosis (AMS). We performed a detailed phenotypic study on the effects of the loss of a single ROP on the establishment of both root symbioses. Moreover, we evaluated the expression of key genes related to CSSP and to the rhizobial-specific pathway. Under our experimental conditions, rop3 mutant showed less nodule formation at 7- and 21-days post inoculation as well as less microcolonies and a higher frequency of epidermal infection threads. However, AMF root colonization was not affected. These results suggest a role of ROP3 as a positive regulator of infection thread formation and nodulation in L. japonicus. In addition, CSSP gene expression was neither affected in NFS nor in AMS condition in rop3 mutant. whereas the expression level of some genes belonging to the rhizobial-specific pathway, like RACK1, decreased in the NFS. In conclusion, ROP3 appears to be involved in the NFS, but is neither required for intra-radical growth of AMF nor arbuscule formation.Ivette García-SotoIvette García-SotoRaphael BoussageonYareni Marlene Cruz-FarfánJesus Daniel Castro-ChilpaLiz Xochiquetzal Hernández-CerezoVictor Bustos-ZagalAlfonso Leija-SalasGeorgina HernándezMartha TorresDamien FormeyPierre-Emmanuel CourtyDaniel WipfMario SerranoAlexandre TromasAlexandre TromasFrontiers Media S.A.articlesymbiotic nitrogen fixationROPrho-GTPaseLotus japonicusarbuscular mycorrhizal symbiosisPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	symbiotic nitrogen fixation ROP rho-GTPase Lotus japonicus arbuscular mycorrhizal symbiosis Plant culture SB1-1110
spellingShingle	symbiotic nitrogen fixation ROP rho-GTPase Lotus japonicus arbuscular mycorrhizal symbiosis Plant culture SB1-1110 Ivette García-Soto Ivette García-Soto Raphael Boussageon Yareni Marlene Cruz-Farfán Jesus Daniel Castro-Chilpa Liz Xochiquetzal Hernández-Cerezo Victor Bustos-Zagal Alfonso Leija-Salas Georgina Hernández Martha Torres Damien Formey Pierre-Emmanuel Courty Daniel Wipf Mario Serrano Alexandre Tromas Alexandre Tromas The Lotus japonicus ROP3 Is Involved in the Establishment of the Nitrogen-Fixing Symbiosis but Not of the Arbuscular Mycorrhizal Symbiosis
description	Legumes form root mutualistic symbioses with some soil microbes promoting their growth, rhizobia, and arbuscular mycorrhizal fungi (AMF). A conserved set of plant proteins rules the transduction of symbiotic signals from rhizobia and AMF in a so-called common symbiotic signaling pathway (CSSP). Despite considerable efforts and advances over the past 20 years, there are still key elements to be discovered about the establishment of these root symbioses. Rhizobia and AMF root colonization are possible after a deep cell reorganization. In the interaction between the model legume Lotus japonicus and Mesorhizobium loti, this reorganization has been shown to be dependent on a SCAR/Wave-like signaling module, including Rho-GTPase (ROP in plants). Here, we studied the potential role of ROP3 in the nitrogen-fixing symbiosis (NFS) as well as in the arbuscular mycorrhizal symbiosis (AMS). We performed a detailed phenotypic study on the effects of the loss of a single ROP on the establishment of both root symbioses. Moreover, we evaluated the expression of key genes related to CSSP and to the rhizobial-specific pathway. Under our experimental conditions, rop3 mutant showed less nodule formation at 7- and 21-days post inoculation as well as less microcolonies and a higher frequency of epidermal infection threads. However, AMF root colonization was not affected. These results suggest a role of ROP3 as a positive regulator of infection thread formation and nodulation in L. japonicus. In addition, CSSP gene expression was neither affected in NFS nor in AMS condition in rop3 mutant. whereas the expression level of some genes belonging to the rhizobial-specific pathway, like RACK1, decreased in the NFS. In conclusion, ROP3 appears to be involved in the NFS, but is neither required for intra-radical growth of AMF nor arbuscule formation.
format	article
author	Ivette García-Soto Ivette García-Soto Raphael Boussageon Yareni Marlene Cruz-Farfán Jesus Daniel Castro-Chilpa Liz Xochiquetzal Hernández-Cerezo Victor Bustos-Zagal Alfonso Leija-Salas Georgina Hernández Martha Torres Damien Formey Pierre-Emmanuel Courty Daniel Wipf Mario Serrano Alexandre Tromas Alexandre Tromas
author_facet	Ivette García-Soto Ivette García-Soto Raphael Boussageon Yareni Marlene Cruz-Farfán Jesus Daniel Castro-Chilpa Liz Xochiquetzal Hernández-Cerezo Victor Bustos-Zagal Alfonso Leija-Salas Georgina Hernández Martha Torres Damien Formey Pierre-Emmanuel Courty Daniel Wipf Mario Serrano Alexandre Tromas Alexandre Tromas
author_sort	Ivette García-Soto
title	The Lotus japonicus ROP3 Is Involved in the Establishment of the Nitrogen-Fixing Symbiosis but Not of the Arbuscular Mycorrhizal Symbiosis
title_short	The Lotus japonicus ROP3 Is Involved in the Establishment of the Nitrogen-Fixing Symbiosis but Not of the Arbuscular Mycorrhizal Symbiosis
title_full	The Lotus japonicus ROP3 Is Involved in the Establishment of the Nitrogen-Fixing Symbiosis but Not of the Arbuscular Mycorrhizal Symbiosis
title_fullStr	The Lotus japonicus ROP3 Is Involved in the Establishment of the Nitrogen-Fixing Symbiosis but Not of the Arbuscular Mycorrhizal Symbiosis
title_full_unstemmed	The Lotus japonicus ROP3 Is Involved in the Establishment of the Nitrogen-Fixing Symbiosis but Not of the Arbuscular Mycorrhizal Symbiosis
title_sort	lotus japonicus rop3 is involved in the establishment of the nitrogen-fixing symbiosis but not of the arbuscular mycorrhizal symbiosis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2f2fa542800d452f9083c8fb1d999e0a
work_keys_str_mv	AT ivettegarciasoto thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT ivettegarciasoto thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT raphaelboussageon thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT yarenimarlenecruzfarfan thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT jesusdanielcastrochilpa thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT lizxochiquetzalhernandezcerezo thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT victorbustoszagal thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT alfonsoleijasalas thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT georginahernandez thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT marthatorres thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT damienformey thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT pierreemmanuelcourty thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT danielwipf thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT marioserrano thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT alexandretromas thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT alexandretromas thelotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT ivettegarciasoto lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT ivettegarciasoto lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT raphaelboussageon lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT yarenimarlenecruzfarfan lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT jesusdanielcastrochilpa lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT lizxochiquetzalhernandezcerezo lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT victorbustoszagal lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT alfonsoleijasalas lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT georginahernandez lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT marthatorres lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT damienformey lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT pierreemmanuelcourty lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT danielwipf lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT marioserrano lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT alexandretromas lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis AT alexandretromas lotusjaponicusrop3isinvolvedintheestablishmentofthenitrogenfixingsymbiosisbutnotofthearbuscularmycorrhizalsymbiosis
_version_	1718431222548398080

The Lotus japonicus ROP3 Is Involved in the Establishment of the Nitrogen-Fixing Symbiosis but Not of the Arbuscular Mycorrhizal Symbiosis

Ejemplares similares