Real-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals.

F-actin remodelling is essential for a wide variety of cell processes. It is important in exocytosis, where F-actin coats fusing exocytic granules. The purpose of these F-actin coats is unknown. They may be important in stabilizing the fused granules, they may play a contractile role and promote exp...

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Autores principales:	Yujin Jang, Carolina Soekmadji, Justin M Mitchell, Walter G Thomas, Peter Thorn
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f309f76c06246c5a4e6ffc21e7971bd
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spelling	oai:doaj.org-article:2f309f76c06246c5a4e6ffc21e7971bd2021-11-18T07:13:33ZReal-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals.1932-620310.1371/journal.pone.0039815https://doaj.org/article/2f309f76c06246c5a4e6ffc21e7971bd2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22768313/?tool=EBIhttps://doaj.org/toc/1932-6203F-actin remodelling is essential for a wide variety of cell processes. It is important in exocytosis, where F-actin coats fusing exocytic granules. The purpose of these F-actin coats is unknown. They may be important in stabilizing the fused granules, they may play a contractile role and promote expulsion of granule content and finally may be important in endocytosis. To elucidate these functions of F-actin remodelling requires a reliable method to visualize F-actin dynamics in living cells. The recent development of Lifeact-EGFP transgenic animals offers such an opportunity. Here, we studied the characteristics of exocytosis in pancreatic acinar cells obtained from the Lifeact-EGFP transgenic mice. We show that the time-course of agonist-evoked exocytic events and the kinetics of each single exocytic event are the same for wild type and Lifeact-EGFP transgenic animals. We conclude that Lifeact-EGFP animals are a good model to study of exocytosis and reveal that F-actin coating is dependent on the de novo synthesis of F-actin and that development of actin polymerization occurs simultaneously in all regions of the granule. Our insights using the Lifeact-EGFP mice demonstrate that F-actin coating occurs after granule fusion and is a granule-wide event.Yujin JangCarolina SoekmadjiJustin M MitchellWalter G ThomasPeter ThornPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e39815 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yujin Jang Carolina Soekmadji Justin M Mitchell Walter G Thomas Peter Thorn Real-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals.
description	F-actin remodelling is essential for a wide variety of cell processes. It is important in exocytosis, where F-actin coats fusing exocytic granules. The purpose of these F-actin coats is unknown. They may be important in stabilizing the fused granules, they may play a contractile role and promote expulsion of granule content and finally may be important in endocytosis. To elucidate these functions of F-actin remodelling requires a reliable method to visualize F-actin dynamics in living cells. The recent development of Lifeact-EGFP transgenic animals offers such an opportunity. Here, we studied the characteristics of exocytosis in pancreatic acinar cells obtained from the Lifeact-EGFP transgenic mice. We show that the time-course of agonist-evoked exocytic events and the kinetics of each single exocytic event are the same for wild type and Lifeact-EGFP transgenic animals. We conclude that Lifeact-EGFP animals are a good model to study of exocytosis and reveal that F-actin coating is dependent on the de novo synthesis of F-actin and that development of actin polymerization occurs simultaneously in all regions of the granule. Our insights using the Lifeact-EGFP mice demonstrate that F-actin coating occurs after granule fusion and is a granule-wide event.
format	article
author	Yujin Jang Carolina Soekmadji Justin M Mitchell Walter G Thomas Peter Thorn
author_facet	Yujin Jang Carolina Soekmadji Justin M Mitchell Walter G Thomas Peter Thorn
author_sort	Yujin Jang
title	Real-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals.
title_short	Real-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals.
title_full	Real-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals.
title_fullStr	Real-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals.
title_full_unstemmed	Real-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals.
title_sort	real-time measurement of f-actin remodelling during exocytosis using lifeact-egfp transgenic animals.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2f309f76c06246c5a4e6ffc21e7971bd
work_keys_str_mv	AT yujinjang realtimemeasurementoffactinremodellingduringexocytosisusinglifeactegfptransgenicanimals AT carolinasoekmadji realtimemeasurementoffactinremodellingduringexocytosisusinglifeactegfptransgenicanimals AT justinmmitchell realtimemeasurementoffactinremodellingduringexocytosisusinglifeactegfptransgenicanimals AT waltergthomas realtimemeasurementoffactinremodellingduringexocytosisusinglifeactegfptransgenicanimals AT peterthorn realtimemeasurementoffactinremodellingduringexocytosisusinglifeactegfptransgenicanimals
_version_	1718423715933323264

Real-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals.

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